一株孔雀源奇异变形杆菌全基因组测序及毒力与耐药性分析

doi:10.16431/j.cnki.1671-7236.2024.07.028

摘要/Abstract

摘要： 【目的】试验旨在了解分离自蓝孔雀的奇异变形杆菌PM19的毒力、耐药性以及基因组特征。【方法】采用动物试验、药敏试验和PCR方法对分离菌进行毒力、耐药性以及毒力基因检测,并运用Illumina NovaSeq6000平台进行基因组框架图测序,通过NR、KEGG、Swiss-Prot、COG、PHI-base、CAZy、CARD和VFDB数据库进行功能基因注释以及病原宿主互作用蛋白、碳水化合物活性酶、耐药基因和毒力因子预测分析。【结果】分离菌PM19为多重耐药菌,对受试的氨苄西林、阿莫西林/克拉维酸钾、头孢曲松、头孢噻肟、阿米卡星、庆大霉素、卡那霉素、环丙沙星、四环素、强力霉素、磺胺嘧啶钠、替米考星、氟苯尼考和氯霉素共14种抗菌药物全部耐药。分离菌具有较强毒力,对小鼠半数致死量为6.19×10⁶ CFU。FliL、zapA、rsmA、hmpA、mrpA、atfA、pmfA和ureC共8种毒力基因在该菌中被检测到。全基因组测序分析显示,该菌基因组大小约为3.98 Mb,GC含量为38.94%,预测到3 715个编码基因。PHI-base数据库注释出158个与病原宿主互作用有关的蛋白基因;CAZy数据库注释出101个碳水化合物活性酶基因;通过CARD数据库和VFDB数据库分别注释到92个耐药基因和8类毒力相关基因。【结论】奇异变形杆菌PM19菌株具有较强毒力且为多重耐药菌株,携带大量病原宿主互作用蛋白基因、碳水化合物活性酶基因、耐药基因及毒力基因。

关键词: 奇异变形杆菌; 多重耐药性; 毒力; 全基因组测序

Abstract: 【Objective】 The aim of this study was to investigate the virulence,drug resistance and genomic characteristics of Proteus mirabilis PM19 isolated from blue peacock.【Method】 Animal experiments,drug sensitivity tests and PCR methods were used to detect the virulence,drug resistance and virulence genes of the isolated bacteria.The Illumina NovaSeq6000 platform was used to sequence the genome frame diagram,and the functional genes,pathogenic host interaction protein,carbohydrate active enzyme,drug resistance gene and virulence factor prediction were carried out by NR,KEGG,Swiss-Prot,COG,PHI-base,CAZy,CARD and VFDB databases.【Result】 The isolate PM19 was a multidrug-resistant bacterium and was resistant to 14 antimicrobial agents including ampicillin,amoxicillin/clavulanate potassium,ceftriaxone,cefotaxime,amikacin,gentamicin,kanamycin,ciprofloxacin,tetracycline,doxycycline,sulfadiazine sodium,tilmicosin,florfenicol and chloramphenicol.The isolated bacteria had strong virulence,and the median lethal dose to mice was 6.19×10⁶ CFU.Eight virulence genes including FliL,zapA,rsmA,hmpA,mrpA,atfA,pmfA,ureC were detected in the strain.The whole genome sequencing analysis showed that the genome size of the strain was about 3.98 Mb,the GC content was 38.94%,and 3 715 coding genes were predicted.158 protein genes related to pathogen-host interaction were annotated by PHI-base database.101 carbohydrate active enzyme genes were annotated by CAZy database.92 drug resistance genes and 8 virulence-related genes were annotated by CARD database and VFDB database,respectively.【Conclusion】 The Proteus mirabilis strain PM19 was virulent and multidrug-resistant,carrying a large number of pathogenic host interaction protein genes,carbohydrate active enzyme genes,drug resistance genes and virulence genes.

Key words: Proteus mirabilis; multi-drug resistant; virulence; whole genome sequencing

中图分类号:

S852.61⁺2

焦凤超, 雷震, 李迎晓, 武娴, 曲哲会. 一株孔雀源奇异变形杆菌全基因组测序及毒力与耐药性分析[J]. 中国畜牧兽医, 2024, 51(7): 3030-3040.

JIAO Fengchao, LEI Zhen, LI Yingxiao, WU Xian, QU Zhehui. Whole Genome Sequencing and Virulence and Drug Resistance Analysis of a Strain of Proteus mirabilis Derived from Peacock[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3030-3040.

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