通辽部分地区鸭源大肠杆菌耐药性检测及多重耐药菌株YA-1全基因组测序分析

doi:10.16431/j.cnki.1671-7236.2024.06.033

摘要/Abstract

摘要： 【目的】大肠杆菌作为家禽最常见细菌性病原之一,其耐药性的不断增强已引起广泛关注。试验旨在了解通辽地区部分鸭场大肠杆菌的耐药性及耐药基因分布,为该地区鸭源大肠杆菌耐药性及耐药基因的研究提供参考。【方法】经16S rRNA测序、生化试验和小鼠致病性试验对送检的通辽市各旗县15份病死鸭肠道样品进行病原菌分离纯化,对分离菌株进行药敏试验及耐药基因的PCR检测,并选取多重耐药菌株进行全基因组测序。【结果】所分离的10株鸭源大肠杆菌均具有不同程度耐药性,对链霉素、环丙沙星、恩诺沙星等药物的耐药率在80%及以上,耐药基因aphA1、strB和qnrS的检出率最高,达到100%。多重耐药菌株YA-1全基因组测序证实,该菌株染色体大小为4 844 827 bp,携带3个完整的质粒,大小分别为144 776 bp(pTLYA-1)、113 584 bp(pTLYA-2)和77 544 bp(pTLYA-3)。分离菌株携带arr-3、aadA16、sul1等70个耐药基因,其中pTLYA-1中携arr-3、dfrA27、aadA16等8个可移动耐药基因,pTLYA-3中携带floR、tetA、sul2等5个可移动耐药基因,pTLYA-2中未发现耐药基因。【结论】由通辽地区部分鸭场分离的鸭源大肠杆菌存在多重耐药性,耐药水平较高,aphA1、strB和qnrS耐药基因普遍流行。

关键词: 鸭; 大肠杆菌; 耐药性; 耐药基因; 全基因组测序

Abstract: 【Objective】 As one of the most common bacterial pathogens in poultry,the increasing resistance of Escherichia coli (E.coli) has attracted wide attention.The purpose of this study was to understand the distribution of drug resistance and drug resistance genes of E.coli in some duck farms in Tongliao area,so as to provide reference for the study of drug resistance and drug resistance genes of E.coli from ducks in this area.【Method】 The pathogenic bacteria were isolated and purified from 15 intestinal samples of dead ducks in each county of Tongliao city by 16S rRNA sequencing,biochemical test and mouse pathogenicity test.The isolated strains were subjected to drug sensitivity test and PCR detection of drug resistance genes,and multi-drug resistant strains were selected for whole genome sequencing.【Result】 All 10 strains of E.coli had different degrees of drug resistance.The resistance rates to streptomycin,ciprofloxacin,ofloxacin and enrofloxacin were all above 80%,and the detection rates of drug resistance genes aphA1, strB and qnrS were 100%.The whole genome sequencing of the multi-drug resistant strain YA-1 confirmed that the chromosome size of the strain was 4 844 827 bp,carrying 3 complete plasmids,which were 144 776 bp (pTLYA-1),113 584 bp (pTLYA-2) and 77 544 bp (pTLYA-3),respectively.The isolated strains carried 70 drug resistance genes such as arr-3,aadA16 and sul1.Among them,pTLYA-1 carried 8 mobile drug resistance genes such as arr-3,dfrA27 and aadA16,pTLYA-3 carried 5 mobile drug resistance genes such as floR,tetA and sul2,and no drug resistance genes were found in pTLYA-2.【Conclusion】 E.coli isolated from some duck farms in Tongliao area had multiple drug resistance,and the resistance level was high.The resistance genes of aphA1,strB and qnrS were prevalent.

Key words: ducks; Escherichia coli; drug resistance; drug resistance genes; whole genome sequencing

中图分类号:

S852.61⁺7

王梓, 孙淼, 王永强, 孟令浩, 耿超, 石金川, 陈伟, 邱军, 刘锴. 通辽部分地区鸭源大肠杆菌耐药性检测及多重耐药菌株YA-1全基因组测序分析[J]. 中国畜牧兽医, 2024, 51(6): 2585-2596.

WANG Zi, SUN Miao, WANG Yongqiang, MENG Linghao, GENG Chao, SHI Jinchuan, CHEN Wei, QIU Jun, LIU Kai. Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2585-2596.

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