规模舍饲羊场羊消化道及环境寄生虫情况调查

doi:10.16431/j.cnki.1671-7236.2023.05.042

摘要/Abstract

摘要： 【目的】调查规模化舍饲羊场及散养羊消化道感染及环境污染寄生虫情况,为中国规模舍饲羊场羊消化道寄生虫综合防控提供参考。【方法】采用离心沉淀法、卢戈氏碘液染色法和饱和蔗糖溶液漂浮法等技术,对采自河南和宁夏的4个规模舍饲羊场及12个散养户的1 025份羊粪便样品寄生虫感染和712份环境样品寄生虫污染情况进行检查,采用麦克马斯特法计数球虫阳性样品每克粪便中的球虫卵囊数(OPG)。选择汝州规模舍饲羊场进行季节动态调查,了解不同季节规模舍饲羊场羊消化道感染及环境污染寄生虫情况。【结果】羊消化道寄生虫总感染率为82.83%(849/1 025),共检出8种(类)寄生虫,分别为球虫、阿米巴、贾第虫、隐孢子虫、绦虫、鞭虫、细颈线虫和其他线虫。优势虫种为球虫(71.90%),其次为阿米巴(49.07%),球虫与阿米巴较易发生混合感染。环境样品寄生虫总阳性率为38.48%(274/712),共检出6种(类)寄生虫,包括球虫、阿米巴、贾第虫、鞭虫、细颈线虫和其他线虫,优势虫种是线虫(22.75%)。河南规模舍饲羊场羊消化道寄生虫感染率以及环境样品寄生虫检出率均显著低于宁夏规模舍饲羊场(P＜0.05)。舍饲羊与散养放牧羊相比,感染的虫种更少、感染率更低;哺乳羔羊消化道寄生虫感染率显著低于其他生理阶段(P＜0.05);在汝州规模舍饲羊场中,冬季羊消化道寄生虫感染率显著低于其他季节(P＜0.05),哺乳羔羊球虫感染率最低(14.95%),但感染强度最大(平均OPG为105 203);育肥期羔羊的感染率最高(88.99%)。【结论】规模化舍饲羊场羊消化道寄生虫感染率较高,球虫和阿米巴混合感染较普遍,环境寄生虫污染严重,应加强卫生措施,防止环境寄生虫污染及病原散播,做好计划性驱虫。

关键词: 规模舍饲羊场; 消化道寄生虫; 环境污染; 感染率

Abstract: 【Objective】 The purpose of this study was to investigate the digestive tract infection and environmental pollution parasites of sheep in large-scale sheep farms and free-range sheep farms,and provide reference for the comprehensive prevention and control of digestive tract parasites of sheep in large-scale sheep farms in China.【Method】 1 025 sheep feces samples and 712 environmental samples collected from 4 scale sheep farms and 12 free-range households in Henan and Ningxia were examined for parasite infection by centrifugation,Lugol’s iodine staining and saturated sucrose solution floatation methods,and the number of Coccidia oocysts per gram of feces (OPG) in coccidia-positive samples was counted by McMaster’s method.The seasonal dynamic survey was conducted in large-scale sheep farms of Ruzhou to understand the digestive tract infection and environmental pollution parasites of sheep farm in different seasons.【Result】 The total infection rate of digestive tract parasites in sheep was 82.83% (849/1 025),and 8 species (classes) of parasites were detected,including Coccidia,Amoeba,Giardia,Cryptosporidium,Moniezia,Whipworm, Nematodirus and Other Nematodes.The dominant worm species was Coccidia (71.90%),followed by Amoeba (49.07%),and Coccidia and Amoeba were more likely to have mixed infections.The total parasite positivity rate of environmental samples was 38.48% (274/712),and 6 species (classes) of parasites were detected,including Coccidia,Amoeba,Giardia,Whipworm,Nematodirus and other Nematodes,with the dominant species being Nematodes (22.75%).Infection rates of digestive tract parasites and parasite detection rates of environmental samples in Henan scale sheep farms were significantly lower than Ningxia scale sheep farms (P＜0.05).Compared with free-ranging sheep,sheep were infected with fewer worm species and lower infection rate.The infection rate of digestive tract parasites in lactating lambs was significantly lower than that of other physiological stages (P＜0.05).In Ruzhou large-scale sheep farms,the infection rate of digestive tract parasites of sheep in winter was significantly lower than that in other seasons (P＜0.05),The infection rate of Coccidia in suckling lambs was the lowest (14.95%),but the infection intensity was the highest (the average OPG was 105 203),the infection rate of fattening lambs was the highest (88.99%).【Conclusion】 The infection rate of digestive tract parasitic in sheep on large-scale sheep farms was higher,the mixed infections of Coccidia and Amoeba were common,and environmental parasitic contamination was serious.Hygiene measures should be strengthened to prevent environmental parasitic contamination and pathogen dispersal,and planned deworming should be done.

Key words: large-scale sheep farms; digestive tract parasites; environmental contamination; infection rate

中图分类号:

S852.7

赵乾明, 齐萌, 徐泽立, 公丕宏, 卢晨阳, 裴志阳, 菅复春, 王建东, 井波, 宁长申. 规模舍饲羊场羊消化道及环境寄生虫情况调查[J]. 中国畜牧兽医, 2023, 50(5): 2156-2165.

ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.

参考文献

[1] 卢艳平,肖海峰.我国居民肉类消费特征及趋势判断——基于双对数线性支出模型和LA/AIDS模型[J].中国农业大学学报,2020,25(1):180-190. LU Y P,XIAO H F.Chinese residents'meatconsumption characteristics and trend judgment:Based on the double-log linear expenditure model and LA/AIDS model[J].Journal of China Agricultural University,2020,25(1):180-190.(in Chinese)
[2] 吴仕琴.舍饲羊养殖及疾病防控[J/OL].畜牧兽医科学(电子版),2022,7:94-95. WU S Q.Prevention and control of diseases in sheep farms[J/OL].Graziery Veterinary Sciences (Electronic Version),2022,7:94-95.(in Chinese)
[3] PINILLA LEÓN J C,DELGADO N U,FLOREZ A A.Prevalence of gastrointestinal parasites in cattle and sheep in three municipalities in the Colombian Northeastern Mountain[J].Veterinary World,2019,12(1):48-54.
[4] 李世杰.球虫荧光定量PCR方法建立及中药抗羊球虫效果评价[D].郑州:河南农业大学,2022. LI S J.Development of a qPCR for detection of Eimeria spp.and anticoccidial effect assessment of traditional Chinese medicine[D].Zhengzhou:Henan Agricultural University,2022.(in Chinese)
[5] 郝桂英.羊球虫病的研究进展[J].中国兽医学报,2017,37(3):577-584. HAO G Y.Advances in coccidiosis of goats and sheep[J].Chinese Journal of Veterinary Science,2017,37(3):577-584.(in Chinese)
[6] JUSZCZAK M,SADOWSKA N,UDAȽA J.Parasites of the digestive tract of sheep and goats from organic farms in Western Pomerania,Poland[J].Annals of Parasitology,2019,65(3):245-250.
[7] 常艳凯.西藏地区羊肠道寄生虫流行病学研究与人兽共患风险分析[D].郑州:河南农业大学,2018. CHANG Y K.Epidemiological study of intestinal parasites and risk analysis of zoonotic in Tibetan sheep in Tibet[D].Zhengzhou:Henan Agricultural University,2018.(in Chinese)
[8] 孔繁瑶.家畜寄生虫学[M].北京:中国农业大学出版社,1981. KONG F Y.Livestock Parasitology[M].Beijing:China Agricultural University Press,1981.(in Chinese)
[9] 李国清.兽医寄生虫学(双语版)[M].北京:中国农业大学出版社,2006. LI G Q.Veterinary Parasitology (Bilingual version)[M].Beijing:China Agricultural University Press,2006.(in Chinese)
[10] 菅忆晨,卢晨阳,王占铭,等.海南三亚黑山羊肠道寄生虫流行病学调查[J].中国兽医杂志,2021,57(12):82-87. JIAN Y C,LU C Y,WANG Z M,et al.Epidemiological investigation of intestinal parasites in Black goats in Sanya,Hainan[J].Chinese Journal of Veterinary Medicine,2021,57(12):82-87.(in Chinese)
[11] 樊峥嵘.江苏省部分地区羊消化道寄生虫感染情况调查及两种绵羊球虫PCR检测方法的建立[D].扬州:扬州大学,2022. FAN Z R.Investigation of intestinal parasites in goats and sheep from partial areas of Jiangsu province and establishment of PCR method for detection of sheep coccidia[D].Yangzhou:Yangzhou University,2022.(in Chinese)
[12] 时文贤,叶红艳.河南省部分地区山羊肠道寄生虫感染情况调查[J].中国动物传染病学报,2021,29(5):115-118. SHI W X,YE H Y.Investigation on intestinal parasites infection of goats in some areas of Henan province[J].Chinese Journal of Animal Infectious Diseases,2021,29(5):115-118.(in Chinese)
[13] 昭日格图,林鑫,毛冉,等.赤峰地区舍饲肉羊消化道寄生虫感染情况调查[J].湖北畜牧兽医,2021,42(12):23-24. ZHAO R G T,LIN X,MAO R,et al.Investigation of digestive tract parasitic infections in housed meat sheep in Chifeng area[J].Hubei Journal of Animal and Veterinary Sciences,2021,42(12):23-24.(in Chinese)
[14] 景纪春,宁晓冬,崔艳艳,等.规模化全舍饲湖羊消化道寄生虫感染情况调查[J].中国畜牧兽医,2019,46(7):2103-2109. JING J C,NING X D,CUI Y Y,et al.Survey prevalence of gastrointestinal parasites in Hu sheep from stall-feeding farms[J] Chinese Animal Husbandry&Veterinary Medicine,2019,46(7):2103-2109.(in Chinese)
[15] 王娟,潘葳,金兰梅,等.舍饲肉羊消化道寄生虫感染情况调查[J].金陵科技学院学报,2015,31(4):89-92. WANG J,PAN W,JIN L M,et al.The investigation of infection in sheep gastrointestinal parasites[J] Journal of Jinling Institute of Technology,2015,31(4):89-92.(in Chinese)
[16] DEY A R,BEGUM N,BISWAS H,et al.Prevalence and factors influencing gastrointestinal parasitic infections in sheep in Bangladesh[J].Annals of Parasitology,2021,67(2):187-194.
[17] 郑双健,李东方,曹乐天,等.甘肃省部分地区藏羊肠道寄生虫感染情况调查[J].中国兽医科学,2018,48(11):1394-1400. ZHENG S J,LI D F,CAO L T,et al.Investigation of intestinal parasite infections in Tibetan sheep in parts of Gansu province[J].Chinese Veterinary Science,2018,48(11):1394-1400.(in Chinese)
[18] 马萍.小尾寒羊羔羊寄生虫病调查及防治效果[J].中国兽医寄生虫病,2007,5:35-37. MA P.Investigation and control effect of parasitic diseases in Small Tail Han lambs[J].Chinese Journal of Veterinary Parasitology,2007,5:35-37.(in Chinese)
[19] 廖党金,骆佳锐,戴卓建,等.养殖场绵羊寄生虫病的控制技术[J].中国兽医学报,2006,4:439-441. LIAO D J,LUO J R,DAI Z J,et al.A control technology of parasitosis in intensive sheep farms[J].Chinese Journal of Veterinary Science,2006,4:439-441.(in Chinese)
[20] 赵姗姗,李玉婉,王坤轮,等.河南开封某场羊肠道寄生虫感染情况调查报告[J].当代畜牧,2019,3:58-61. ZHAO S S,LI Y W,WANG K L,et al.Investigation on intestinal parasitic infection of sheep in Kaifeng,Henan province[J].Contemporary Animal Husbandry,2019,3:58-61.(in Chinese)
[21] 冯博,金振华,张备,等.羊球虫病的防治与诊断措施[J].现代畜牧兽医,2022,5:82-85. FENG B,JIN Z H,ZHANG B,et al.Prevention and diagnosis of sheep coccidiosis[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2022,5:82-85.(in Chinese)
[22] 李梦婕,彭永帅,朱丹,等.绵羊肠道寄生虫感染情况调查[J].中国畜牧兽医,2013,40(4):201-205. LI M J,PENG Y S,ZHU D,et al.Survey on the prevalence of intestinal parasites in sheep[J].China Animal Husbandry&Veterinary Medicine,2013,40(4):201-205.(in Chinese)
[23] 王占铭.不同品种绵羊球虫种类鉴定及5种药食同源中药抗羊球虫效果评价[D].郑州:河南农业大学,2022. WANG Z M.Identification of different sheep coccidia species and evaluation of anti-coccidia effect of 5 medicinal and edible Chese herbs[D].Zhengzhou:Henan Agricultural University,2022.(in Chinese)