中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (1): 125-133.doi: 10.16431/j.cnki.1671-7236.2023.01.013

• 营养与饲料 • 上一篇    下一篇

补喂L-精氨酸对返情纯血母马妊娠率及血浆参数的影响

臧长江1, 刘保宣1, 院东2, 李晓斌1, 李凤鸣1, 陈开旭1, 米热古丽·伊马木1, 杨开伦1   

  1. 1. 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养实验室, 乌鲁木齐 830052;
    2. 昌吉市吉缘牧业有限公司, 昌吉 831100
  • 收稿日期:2022-06-16 出版日期:2023-01-05 发布日期:2023-01-04
  • 作者简介:臧长江,E-mail:zcj780@126.com;刘保宣,E-mail:18313008557@163.com。
  • 基金资助:
    新疆维吾尔自治区重大科技专项"马产业升级技术创新工程"(2017A01002)

Effect of Supplementation with L-arginine on Pregnancy Rate and Plasma Parameters of Re-service Thoroughbred Horses

ZANG Changjiang1, LIU Baoxuan1, YUAN Dong2, LI Xiaobin1, LI Fengming1, CHEN Kaixu1, MIREGULI·Yimamu1, YANG Kailun1   

  1. 1. Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Changji Jiyuan Animal Husbandry Co., Ltd., Changji 831100, China
  • Received:2022-06-16 Online:2023-01-05 Published:2023-01-04

摘要: 【目的】试验旨在研究补喂L-精氨酸对返情纯血马妊娠率及血液参数的影响。【方法】选择返情纯血母马14匹随机分为2组,每组7匹(n=7),分别为对照组、L-精氨酸组,对照组饲喂基础饲粮,L-精氨酸组在基础饲粮中添加50 g/d L-精氨酸;预饲期7 d,试验期30 d。返情母马发情时,第一次人工输精结束当天补喂L-精氨酸;配种结束时直肠B超检查妊娠情况,统计妊娠率,分别于试验第0、15、30天颈静脉采血,用于测定总一氧化氮合成酶(total nitric oxide synthetase,TNOS)、内皮型一氧化氮合成酶(endothelial nitric oxide synthetase,eNOS)活性及一氧化氮(nitric oxide,NO)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、生长激素(growth hormone,GH)、胰岛素样生长因子(insulinlike growth factor,IGF)含量等指标。【结果】与对照组相比,L-精氨酸组妊娠率显著提高(P<0.05);试验第0、15、30天血浆TNOS、eNOS、鸟氨酸脱羧酶(orinithine decarboxylase,ODC)活性及NO、IGF-Ⅰ、IGF-Ⅱ、VEGF含量在两组间均无显著差异(P>0.05);试验第30天L-精氨酸组血浆氨(ammonia,NH3)、尿素氮(urea nitrogen,UN)含量分别比对照组降低13.49%、26.91%(P<0.05)。与对照组相比,试验第30天L-精氨酸组血浆GH含量提高50.26%(P<0.01),试验第0、15、30天两组间母马血浆免疫球蛋白A (IgA)、免疫球蛋白M (IgM)、免疫球蛋白G (IgG)含量均无显著差异(P>0.05)。【结论】返情纯血母马补喂L-精氨酸能显著提高妊娠率,改变母马体循环血浆UN、NH3、GH含量。L-精氨酸可作为营养调控剂提高纯血母马妊娠率。

关键词: L-精氨酸; 母马; 妊娠率; 血浆参数

Abstract: 【Objective】 The purpose of the experiment was to study the effect of supplementation with L-arginine on pregnancy rate and plasma parameters of re-service thoroughbred horses.【Method】 14 re-service thoroughbred horses were randomly divided into 2 groups, 7 in each group(n=7), as the control group and L-arginine group.The control group was fed with basic diet, and the L-arginine group was added with 50 g/d L-arginine on the basis of basic diet.The pre feeding period was 7 d, and the trial period was 30 d.When the returning mare was in estrus, L-arginine was supplemented on the day after the first artificial insemination.At the end of mating, the pregnancy was examined by rectum B-ultrasound, and the pregnancy rate was calculated.Blood was collected from the jugular vein of mares at 0, 15 and 30 d respectively.The activities of total nitric oxide synthase (TNOS), endothelial nitric oxide synthase (eNOS) and the contonts of nitric oxide (NO), vascular endothelial growth factor (VEGF), growth hormone (GH), insulin-like growth factor(IGF) were measured.【Result】 Compared with the control group, the pregnancy rate of the L-arginine group was significantly increased (P<0.05).There was no significant difference in plasma TNOS, eNOS, NO, ODC, IGF-Ⅰ, IGF-Ⅱ and VEGF levels/activities between the two groups at 0, 15 and 30 d (P>0.05).On the 30th day of the test, the plasma ammonia (NH3) and urea nitrogen (UN) contents in the L-arginine group decreased by 13.49% and 26.91% respectively compared with the control group (P<0.05).Compared with the control group, the plasma GH content on the 30th day of the test was significantly increased by 50.26% in L-arginine group (P<0.01).And there was no significant difference in the contents of IgA, IgM and IgG of mares on the 0th, 15th, and 30th days of the test between two groups (P>0.05).【Conclusion】 Feeding L-arginine to the re-service thoroughbred horses could significantly increase the pregnancy rate and change the contents of UN, NH3 and GH in the circulating plasma of the mares.L-arginine could be used as a nutritional regulator to improve the pregnancy rate of thoroughbred mares.

Key words: L-arginine; mare; pregnancy rate; plasma parameters

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