马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响

doi:10.16431/j.cnki.1671-7236.2022.07.034

摘要/Abstract

摘要： 【目的】探究马兜铃酸Ⅰ（aristolochic acid Ⅰ，AA-Ⅰ）对大鼠五脏（心脏、肝脏、脾脏、肺脏和肾脏）的毒性及功能影响，为今后马兜铃属类中药临床用药提供参考。【方法】将SD大鼠随机分为AA-Ⅰ给药组和空白对照组，给药组按20 mg/kg灌服AA-Ⅰ，连续7 d。于给药后0.25、0.5、1、2、24 h及停药后15 d，采集大鼠五脏组织。利用HPLC法检测各个时间点五脏组织中的AA-Ⅰ及马兜铃内酰胺Ⅰ（aristolactams-Ⅰ，AL-Ⅰ）含量，并检测大鼠五脏主要生理功能及病理组织学变化。【结果】给药后0.25 h，AA-Ⅰ主要分布于心脏、肝脏、脾脏和肺脏，同时在肝脏和脾脏中检测到AL-Ⅰ；给药后0.5 h，AA-Ⅰ主要分布于心脏、肝脏、脾脏、肺脏和肾脏，除心脏外，其余四脏中AA-Ⅰ含量均达到高峰值，且在肝脏、肺脏、肾脏中检测到AL-Ⅰ；给药后1 h，AA-Ⅰ主要分布于肝脏和肺脏，且在肝脏中检测到AL-Ⅰ；给药后2 h，AA-Ⅰ主要分布于心脏、肝脏、肺脏和肾脏，且在肾脏中检测到AL-Ⅰ；给药后24 h，AA-Ⅰ主要分布于心脏和肝脏，且在肝脏中检测到AL-Ⅰ；停药后15 d，五脏中均未检测到AA-Ⅰ及AL-Ⅰ。功能检测结果显示，大鼠灌服AA-Ⅰ可引起五脏发生氧化应激损伤。【结论】连续7 d给大鼠灌服20 mg/kg AA-Ⅰ，在五脏中均可检测到不同含量的AA-Ⅰ，且在肝脏、肾脏、肺脏及脾脏中检测到AL-Ⅰ。AA-Ⅰ可对五脏造成不同程度的损伤，其损伤与氧化应激有关。

关键词: 马兜铃酸Ⅰ(AA-Ⅰ); 马兜铃内酰胺Ⅰ(AL-Ⅰ); 大鼠; 五脏; 毒性

Abstract: 【Objective】 This study was aimed to explore the toxicity and functional effects of aristolochic acid Ⅰ (AA-Ⅰ) on the five viscera (heart,liver,spleen,lung and kidney) of rats,so as to provide reference for the clinical use of traditional Chinese medicines of the Aristolochia genus in the future.【Method】 SD rats were randomly divided into aristolochic acid administration and blank control groups.The rats in administration group were gavaged with AA-Ⅰ at 20 mg/kg for 7 days.At 0.25 h,0.5 h,1 h,2 h,24 h,and 15 d after drug withdrawal,the five viscera of rats were taken.HPLC method was used to detect the contents of AA-Ⅰ and aristolactams-Ⅰ (AL-Ⅰ) in five viscera of rats at each time point,and the main physiological functions and histopathological changes of five viscera of rats were detected.【Result】 At 0.25 h after administration,AA-Ⅰ was mainly distributed in heart,liver,spleen and lung,and AL-Ⅰ was detected in liver and spleen.At 0.5 h after administration,AA-Ⅰ was mainly distributed in the heart,liver,spleen,lung and kidney.Except for the heart,the content of AA-Ⅰ in the other four viscera reached a high peak,and AL-Ⅰ was detected in liver,lung and kidney.At 1 h after administration,AA-Ⅰ was mainly distributed in liver and lung,and AL-Ⅰ was detected in liver.At 2 h after administration,AA-Ⅰ was mainly distributed in heart,liver,lung and kidney,and AL-Ⅰ was detected in kidney.At 24 h after administration,AA-Ⅰ was mainly distributed in heart and liver,and AL-Ⅰ was detected in liver.After 15 d of drug withdrawal,AA-Ⅰ and AL-Ⅰ were not detected in five viscera.The results of function tests showed that AA-Ⅰ could cause oxidative stress injury in five viscera.【Conclusion】 AA-Ⅰ was administered to rats at a dose of 20 mg/kg for 7 d, different levels of AA-Ⅰ were detected in five viscera,and AL-Ⅰ was detected in liver,kidney,lung and spleen.AA-Ⅰ could cause different degrees of damage to five viscera,and its damage was related to oxidative stress.

Key words: aristolochic acid Ⅰ (AA-Ⅰ); aristolactams-Ⅰ(AL-Ⅰ); rats; five viscera; toxicity

中图分类号:

S853.7

汪瑶, 燕鸽, 刘智慧, 王玉, 辛全超, 曹新悦, 于文会, 姜晓文. 马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响[J]. 中国畜牧兽医, 2022, 49(7): 2757-2767.

WANG Yao, YAN Ge, LIU Zhihui, WANG Yu, XIN Quanchao, CAO Xinyue, YU Wenhui, JIANG Xiaowen. Study on the Toxicity and Function Effects of Aristolochic Acid Ⅰ on the Five Viscera of Rats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2757-2767.

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