基于转录组测序鉴定猪肌内脂肪沉积相关生物学通路及候选基因

doi:10.16431/j.cnki.1671-7236.2021.12.019

摘要/Abstract

摘要： 本研究主要从转录组的角度对猪肌内脂肪(intramuscular fat,IMF)含量可能的沉积机理进行研究,以期找到IMF沉积相关的通路及候选基因。选择IMF含量差异较大的全同胞个体进行双末端链特异性转录组测序,并利用edgeR软件筛选相关基因;应用ClusterProfiler软件对差异基因进行GO功能和KEGG通路富集分析,并对其进行分子模块(MCODE)分析,挖掘IMF相关候选基因。结果显示,共筛选出474个差异表达基因,其中329个显著上调,145个显著下调,富集参与20个生物学过程。结合GO网络分析,共筛选出15个与IMF相关过程,主要有离子跨膜转运的调节、葡萄糖跨膜转运膜电位的调节和脂肪酸代谢过程等。MCODE分析结果发现,差异表达基因PPP1CB、CLCA1、COX4I1、SGK1、PAK6、PC、KRAS、PAK6、MAST1、VWC2、CACNG家族基因(尤其PPP1CB、CLCA1、COX4I1、KRAS及CACNG家族基因)可能是调节IMF的关键基因,可作为IMF候选基因进一步研究。本研究鉴定出了IMF作用新通路,并筛选出IMF相关候选基因,可为IMF的沉积机理研究提供材料,为优质肉品质猪的育种提供参考。

关键词: 猪; 肌内脂肪; 沉积; 转录组测序

Abstract: The aim of this study was using transcriptome to analyze the deposition mechanism of porcine intramuscular fat (IMF) content, in order to find the pathways and candidate genes related to IMF deposition.The whole sib individuals with large differences in IMF content were selected for double terminal chain specific transcriptome sequencing, and the related genes were screened by edgeR software.ClusterProfiler software was used to perform GO function enrich and KEGG pathway analysis on differential genes, and molecular module (MCODE) analysis was used to mine the intramuscular fat-related candidate genes.The results showed that a total of 474 differentially expressed genes were screened, of which 329 were significantly up-regulated and 145 were significantly down-regulated, enriched and involved in 20 biological processes.Combined with GO network analysis, a total of 15 IMF-related processes were screened, mainly including:Regulation of ion transmembrane transport, regulation of glucose transmembrane transport membrane potential and fatty acid metabolism.MCODE analysis results showed that the differentially expressed genes PPP1CB, CLCA1, COX4I1, SGK1, PAK, PC, KRAS, MAST1, VWC2, CACNG family genes, especially PPP1CB, CLCA1, COX4I1, KRAS and CACNG family genes, might be key genes for regulating IMF, and could be used as IMF candidate gene for further study.In conclusion, this study had identified some new pathways for the action of IMF and screened out candidate genes related to IMF content, which could provide materials for the study of IMF deposition mechanism and reference for the breeding of high-quality meat quality in pigs.

Key words: pigs; IMF; deposition; transcriptome sequencing

中图分类号:

S813.3

孙菁希, 逄世龙, 孙敬春, 褚瑰燕. 基于转录组测序鉴定猪肌内脂肪沉积相关生物学通路及候选基因[J]. 中国畜牧兽医, 2021, 48(12): 4508-4519.

SUN Jingxi, PANG Shilong, SUN Jingchun, CHU Guiyan. Identification of Biological Pathways and Candidate Genes Related to Porcine Intramuscular Fat Deposition Using Transcriptome Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4508-4519.

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