大肠杆菌转座子及整合子携带类型与其多重耐药谱型相关性的研究

doi:10.16431/j.cnki.1671-7236.2020.05.030

摘要/Abstract

摘要： 为探究猪源大肠杆菌转座子及整合子携带类型与其多重耐药相关性，试验采集贵州省12个规模化养猪场猪肛门拭子，分离鉴定到259株大肠杆菌。采用微量肉汤稀释法测定分离菌株对7类（16种）抗菌药物的最小抑菌浓度（MIC）值，并用PCR方法检测转座子及整合子的携带情况。结果发现，受试菌株对7类（16种）抗菌药物呈现出不同程度的耐药，对大观霉素、庆大霉素、四环素为高度耐药，MIC₉₀的耐药倍数为32倍；对亚胺培南、多黏菌素B表现敏感，MIC₉₀的耐药倍数为2倍，其他药物的耐药倍数介于二者之间。受试菌株多重耐药最高达7重，2重以上耐药率达到100%，多重耐药谱型共计14种，以β-内酰胺类+氨基糖苷类+四环素类+酰胺醇类+磺胺类+氟喹诺酮类、β-内酰胺类+氨基糖苷类+四环素类+酰胺醇类+磺胺类+氟喹诺酮类+多肽类为主，占56.97%。受试菌株转座子和整合子检出率较高，转座子及整合子表型共计20种，其中IScp1+IS26+tnpA+tnp513+intI1和IS26+tnpA+tnp513+intI1的组合类型占比最多，分别为48.48%和26.06%。本试验结果表明，插入元件IScp1与β-内酰胺类药物耐药呈显著或极显著相关（P<0.05，P<0.01），插入元件IS26与氨苄西林耐药相关性极显著（P<0.01），与磺胺异唑耐药相关性显著（P<0.05）；整合子intI1与氨苄西林耐药相关性极显著（P<0.01）；intI2与头孢他啶耐药相关性极显著（P<0.01），与头孢噻呋、恩诺沙星、亚胺培南耐药相关性显著（P<0.05）；IScp1+IS26+tnpA+tnp513+intI1组合类型与复方新诺明耐药相关性极显著（P<0.01）；IS26+tnpA+tnp513+intI1组合类型与复方新诺明耐药相关性显著（P<0.05），与头孢噻呋耐药相关性极显著（P<0.01），但与庆大霉素耐药呈负相关关系（P<0.05）。综上，受试菌株对β-内酰胺类、氟喹诺酮类、磺胺类、氨基糖苷类的多重耐药与转座子及整合子携带类型呈显著或极显著相关。

关键词: 大肠杆菌; 转座子; 整合子; 耐药性

Abstract: To investigate the correlation between transposon and integron carrying and multiple drug resistance of Escherichia coli,259 strains of Escherichia coli were isolated from 12 large-scale pig farms by collecting anal swabs.Minimum inhibitory concentration (MIC) values of isolated strains against 7 classes (16 kinds) of antibacterial drugs were measured by microbroth dilution method,and the carrying of transposons and integrons was detected by PCR.The results showed that the tested strains showed different degrees of resistance to 7 classes (16 kinds) of antibacterial drugs.It was highly resistant to spectinomycin,gentamicin and tetracycline,and the drug resistance multiple of MIC₉₀ was 32 times.It was sensitive to imipenem and polymyxin B,and the drug resistance multiple of MIC₉₀ was 2 times,while the resistance of other drugs was between them.The multiple drug resistance up to 7 times,2 times resistant up to 100%.There were 14 types of multidrug resistance spectrum,mainly including β-lactams+aminoglycosides+tetracyclines+amidoalcohols+sulfonamides+fluoroquinolones,β-lactams+aminoglycosides+tetracyclines+amidoalcohols+sulfonamides+fluoroquinolones+peptides,accounting for 56.97%.The detection rate of transposons and integrons was higher.There were 20 types of transposon and integron phenotypes.Among them,the combination types of IScp1+IS26+tnpA+tnp513+intI1 and IS26+tnpA+tnp513+intI1 accounted for the most,48.48% and 26.06%,respectively.The results showed that insertion element IScp1 was significantly correlated with β-lactam drug resistance (P<0.05,P<0.01).The insertion element IS26 was extremely significantly correlated with ampicillin resistance (P<0.01) and significantly correlated with sulfadioxazole resistance (P<0.05).Integron intI1 was extremely significantly correlated with ampicillin resistance (P<0.01),IntI2 was extremely significantly correlated with cefetadime resistance (P<0.01),and significantly correlated with cefetifur,enoxacin and imipenem resistance (P<0.05).The dominant combination type IScp1+IS26+tnpA+tnp513+intI1 was extremely significantly correlated with the drug resistance of compound sulfamethoxazole (P<0.01).The combination type of IS26+tnpA+tnp513+intI1 was extremely significantly correlated with the drug resistance of compound sulfamethoxazole (P<0.05) and was extremely significantly correlated with ceftifur resistance (P<0.01),but it was negatively correlated with gentamicin resistance (P<0.05).The multidrug resistance of the tested strains to β-lactams,fluoroquinolones,sulfonamide and aminoglycosides were significantly or extremely significantly correlated with transposon and integron carrying type.

Key words: Escherichia coli; transposon; integron; drug resistance

中图分类号:

S859.7

林习, 吕世明, 谭艾娟, 罗致茜, 杨睿智, 郑汝青, 周玲. 大肠杆菌转座子及整合子携带类型与其多重耐药谱型相关性的研究[J]. 中国畜牧兽医, 2020, 47(5): 1547-1559.

LIN Xi, LYU Shiming, TAN Aijuan, LUO Zhiqian, YANG Ruizhi, ZHENG Ruqing, ZHOU Ling. Correlation Between Transposon and Integron Carrying Type and Multidrug Resistance Spectrum of Escherichia coli[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1547-1559.

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