四甲基吡嗪对獭兔单增李斯特菌感染和天然免疫的影响

doi:10.16431/j.cnki.1671-7236.2020.05.028

摘要/Abstract

摘要： 本试验通过研究四甲基吡嗪（TMP）对单增李斯特菌感染獭兔载菌量、溶血素、促炎因子（TNF-α、IL-1β和IFN-γ）、免疫器官指数和天然抗体的影响，旨在探讨TMP对单增李斯特菌的抑菌作用。选择断奶獭兔180只随机分为5组，每组6个重复，每个重复6只。对照组饲喂基础日粮，其他4组在基础日粮中分别添加0、50、100、150 mg/kg TMP。饲养试验第1天，4个试验组獭兔每只灌服1 mL（10⁷ CFU）单增李斯特菌株，对照组灌服无菌株的培养液，饲养试验持续14 d。结果表明，TMP降低了獭兔肝脏、脾脏和淋巴结的单增李斯特菌载菌量（P<0.05）。盲肠食糜、肝脏、脾脏和淋巴结载菌量均呈线性下降（P<0.05）。添加TMP使獭兔李斯特菌溶血素和促炎因子（TNF-α和IL-1β）显著降低（P<0.05），14 d时，李斯特菌溶血素呈线性下降（P<0.05），促炎因子（TNF-α和IL-1β）均呈现线性和平方下降（P<0.05）。胸腺指数、脾脏指数、血清IgA和IgG在单增李斯特菌感染后下降，随TMP添加量增加而增加，但只有饲喂至7 d时的脾脏指数呈线性上升（P<0.05）。说明日粮中添加TMP可以降低单增李斯特菌感染獭兔组织载菌量，减少单增李斯特菌的毒力，对提升免疫器官指数和天然抗体有积极作用。

关键词: 四甲基吡嗪; 单增李斯特菌; 载菌量; 天然抗体; 獭兔

Abstract: The study aimed to investigate the effect of tetramethylpyrazine(TMP) supplementation on Listeria monocytogenes loads,listeriolysin O (LLO),proinflammatory factors (TNF-α,IL-1β and IFN-γ),immune organ index and natural immunoglobulins of Rex rabbits infected with Listeria monocytogenes.A total of 180 Rex rabbits were randomly assigned to 5 groups with 6 replicates of 6 rabbits.The control group was fed with the basal diet,and the other four groups were fed with the addition of TMP at 0,50,100 and 150 mg/kg of diet,respectively.Each Rex rabbit in the four treatments was fed with 1 mL (10⁷ CFU) of Listeria monocytogenes,and the control group was fed with the culture medium of aseptic strainat first day of feeding trial.The trial lasted for 14 days.The results showed that TMP addition decreased the loads of Listeria monocytogenes in the spleen,liver and lymph nodes (P<0.05).The pathogen loads in caecal content,liver,spleen and lymph nodes linearly decreased (P<0.05).Also,TMP decreased serum LLO levels and proinflammatory factors TNF-α and IL-1β (P<0.05).Linearly decreased for LLO and linearly and quadratically decreased for TNF-α and IL-1β (P<0.05) at 14 d.The indexes of spleen and liver and the serum levels of IgA and IgG inhanced with the increasing doses of TMP,but the spleen index on 7 d post administration linearly decreased (P<0.05).In conclusion,supplemental TMP in the rabbit diet could attenuate Listeria monocytogenes infection by reducing the pathogen loads and virulence,increasing immune organ indexes and natural immunoglobulins.

Key words: TMP; Listeria monocytogenes; bacterial load; innate immunoglobulins; Rex rabbit

中图分类号:

S829.1

郑江平, 刘宁, 白雪瑞, 张飞可. 四甲基吡嗪对獭兔单增李斯特菌感染和天然免疫的影响[J]. 中国畜牧兽医, 2020, 47(5): 1531-1538.

ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Tetramethylpyrazine on Listeria monocytogenes Infection and Innate Immunity of Rex Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1531-1538.

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