猫泛白细胞减少症病毒的分离与鉴定

›› 2008, Vol. 1 ›› Issue (10): 89-92.

猫泛白细胞减少症病毒的分离与鉴定

亢文华,赵凤龙,郝霖雨,潘春刚

中牧实业股份有限公司技术中心,北京 100070

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20

Isolation and Indentification of Feline Panlenkopenia Virus

KANG Wenhua, ZHAO Fenglong, HAO Linyu, PAN Chungang

Technology & Research Center, China Animal Husbandry Industry Co Ltd, Beijing 100070,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

摘要/Abstract

摘要： 从临床表现有体温升高、呕吐、血样腹泻、脱水等症状的疑似猫泛白细胞减少症感染的病例采取粪样28份。从粪便样品中成功分离获得了7株猫泛白细胞减少症病毒（FPV）：JX-1、JX-2、JX-3、JX-4、JX-5、JX-6和JX-7；应用F81细胞增毒，盲传至3代时在F81细胞上产生细胞病变(脱落、变形、游离等)；核酸型鉴定证明，FPV毒株的代谢可被5-IUDR所抑制，其核酸属于DNA型；所分离的病毒培养物能凝集猪的红细胞（凝集效价达2⁶～2⁸），并能被标准FPV阳性血清所抑制；电镜观察病毒粒子外观呈圆形或六边形，直径20～30 nm；该病毒耐酸、耐热、耐乙醚；动物致病性试验，经口感染分离细胞培养毒1 ml，试验组幼猫第7 d发病，采集病猫粪样做HA试验为阳性反应，做HI试验，其凝集猪红细胞的能力被抑制。

关键词: 猫泛白细胞减少症病毒; 分离; 鉴定

Abstract: Twenty-eight feces samples were collected from feline panleukopenia virus (FPV) cases which had got temperature high, vomiting, diarrhea with blood and dehydration. Seven feline panleukopenia virus strains were isolated from feces samples. They were named JX-1,JX-2,JX-3,JX-4,JX-5,JX-6 and JX-7. F81 cell line on which the FPV was transferred of the third culture were observed cytopathic effect (CPE) such as defluxion, disfiguration and liberation. FPV strain belonged to DNA virus by nucleic acid type analysis ,in which the metabolism of FPV strain was suppressed by 5-IUDR. The isolated virus strain could agglutinate red blood cell of porcine (agglutination titer was from 2⁶ to 2⁸) and it was inhibited by the FPV positive serum. Virion was round or hexagon, and its diameter was approximately from 20 nm to 30 nm by the electronic microscope. Animal experiment indicated the experimental kitty appeared disease about seven days after it inoculated with 1ml virus culture solution. HA and HI detections were positive from the feces sample of ill kitty.

Key words: feline panleukopenia virus; isolate; identify

中图分类号:

S852.65+9.2

亢文华;赵凤龙;郝霖雨;潘春刚. 猫泛白细胞减少症病毒的分离与鉴定[J]. , 2008, 1(10): 89-92.

KANG Wenhua;ZHAO Fenglong;HAO Linyu;PAN Chungang. Isolation and Indentification of Feline Panlenkopenia Virus [J]. , 2008, 1(10): 89-92.

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