自制貉源抗血清治疗犬瘟热病的研究

›› 2010, Vol. 37 ›› Issue (7): 143-145.

自制貉源抗血清治疗犬瘟热病的研究

张立恒1，张智明1，谭婷婷2，张彦龙2

（1.哈药集团生物疫苗有限公司研发中心，哈尔滨 150069 ；2.东北林业大学野生动物资源学院，哈尔滨 150040）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20

The Study of Self-made Anti-serum Being Used to CureCanine Distemper that was Made from Raccoon

ZHANG Li-heng1, ZHANG Zhi-ming1, TAN Ting-ting2,ZHANG Yan-long2

(1.Harbin Pharmaceutical Group Bio-Vaccine Co. Ltd， Harbin 150069,China；2.College of Wildlife Resources, Northeast Forestry University， Harbin 150040，China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

摘要/Abstract

摘要： 应用含有104.5 TCID50/0.1 mL犬瘟热弱毒培养物4 mL免疫特种经济动物乌苏里貉，10 d后采集血液分离血清，病毒用200TCID50与等量连续倍比稀释的血清和经饱和硫酸铵粗提的免疫球蛋白做中和试验鉴定抗体的效价，并对上述2种抗体效价进行比较。结果表明，2917倍稀释全血清、2344倍稀释饱和硫酸铵粗提的免疫球蛋白能保护50% Vero细胞不出现CPE。临床应用全血清治疗24只患犬瘟热病的白貉，22只成活。由此说明可以应用狐貉等特种动物制备的抗血清治疗犬瘟热病。

关键词: 犬瘟热病毒; 抗血清; 治疗

Abstract: In this study, 4 mL canine distemper virus being 104.5 TCID50/0.1 mL, was used to immunize wusuli-raccoon dog which was strain economic animal. After 10 days, animal blood was collected, while blood serum was disassociated. Neutralization test was made to evaluate the potency of antibody, during this test, virus was 200TCID50 , blood serum whose volume was equal with virus was diluted by times after times, while immunoglobulin was extracted crudely by saturated ammonium sulfate. The results clearly suggested that under the project that the blood serum was diluted for 2917 times, the immunoglobulin which was extracted crudely by saturated (NH3)2SO4 was diluted for 2344 times, there was 50% Vero cytoprotection without CPE. 24 white raccoon dogs with this disease were therapied by the whole serum above, and 22 ones was survival, therefore,we demonstrated that antiserum, made from strain animal such as fox, could be used tocure canine distemper virus.

Key words: canine distemper virus; antiserum; therapy

中图分类号:

S858.92

张立恒;张智明;谭婷婷;张彦龙. 自制貉源抗血清治疗犬瘟热病的研究[J]. , 2010, 37(7): 143-145.

ZHANG Li-heng;ZHANG Zhi-ming;TAN Ting-ting;ZHANG Yan-long. The Study of Self-made Anti-serum Being Used to CureCanine Distemper that was Made from Raccoon[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(7): 143-145.

[1]	秦梦可, 李慧鑫, 韩慧, 孟庆大, 谢闪闪. 犬肿瘤免疫治疗性抗体研究进展及方向[J]. 中国畜牧兽医, 2025, 52(5): 2432-2441.
[2]	杨帆, 周俊, 李绮婷, 林巧儿, 梁维佳, 蔡仕楷, 覃丽梅. 除湿利肺消毒散对鸡传染性支气管炎的预防和治疗效果探究[J]. 中国畜牧兽医, 2025, 52(1): 481-490.
[3]	邱基程, 杨宇欣, 张璐, 温泽宇, 陈苏蒙, 曹兴元. 质谱技术在兽用治疗性蛋白药物表征和定量分析上的应用[J]. 中国畜牧兽医, 2024, 51(6): 2319-2329.
[4]	高庆羽, 符乐, 石玉祥, 李杰峰, 刘嫣然, 秦立佳, 张华莹, 何团拥, 王晓芳. 中药组方对大肠杆菌致小鼠腹泻治疗效果研究[J]. 中国畜牧兽医, 2024, 51(6): 2661-2670.
[5]	刘丁语, 何振文, 刘宝玲, 王刚, 王晓虎, 谭臣, 蔡汝健. 戊型肝炎病毒研究进展[J]. 中国畜牧兽医, 2024, 51(2): 736-747.
[6]	甘靖宇, 孙嘉徐, 邓雨晴, 刘波, 俞峰. 雪貂常见肿瘤病的诊疗进展[J]. 中国畜牧兽医, 2024, 51(10): 4635-4642.
[7]	罗世民, 李中波. 怀化地区犬瘟热病毒H、F基因遗传多样性及其遗传进化分析[J]. 中国畜牧兽医, 2023, 50(8): 3248-3257.
[8]	杨志, 余佳欣, 杨林富, 王康, 李冠宗, 段纲, 杨福梅, 刘知奇, 周可, 和君, 代飞燕. 四逆汤加减对仔猪腹泻治疗效果研究[J]. 中国畜牧兽医, 2023, 50(4): 1707-1717.
[9]	江蓝, 杨洛, 路心怡, 任虹霖, 王炜, 俞峰, 刘波. 犬猫心力衰竭治疗药物研究进展[J]. 中国畜牧兽医, 2023, 50(10): 4252-4260.
[10]	赵静, 褚颖, 罗均, 郭霄峰. 嵌合犬瘟热病毒H基因的重组狂犬病病毒的构建及免疫原性研究[J]. 中国畜牧兽医, 2022, 49(12): 4776-4785.
[11]	刘健, 白艺兰, 鞠厚斌, 潘子豪, 俞向前, 杨显超, 朱晓英, 葛菲菲, 葛杰, 于讳茹, 陶田谷晟, 王建, 赵洪进. 犬瘟热病毒SH202003株全基因组序列分析[J]. 中国畜牧兽医, 2021, 48(7): 2559-2568.
[12]	缴莹, 季玲西, 黄薇, 肖盟, 吕艳丽. 1例猫假足菌肿的诊断与治疗[J]. 中国畜牧兽医, 2021, 48(3): 1141-1147.
[13]	王安田, 黄靖, 孙瑜凡, 宋炳晓, 谭臣, 黄琦, 周锐, 黎璐. 猪β防御素-2对猪源肠外致病性大肠杆菌的体内外抑菌活性研究[J]. 中国畜牧兽医, 2021, 48(2): 726-735.
[14]	徐茜, 杨莎, 郝海生, 杜卫华, 庞云渭, 赵善江, 邹惠影, 朱化彬, 李树静, 余文莉, 赵学明. 单碱基编辑技术的应用研究进展[J]. 中国畜牧兽医, 2021, 48(12): 4403-4411.
[15]	林昌杰, 李婷, 唐鑫, 陈颖钰, 郭爱珍, 胡长敏. 上皮间质转化促乳腺癌机制及药物干预治疗研究进展[J]. 中国畜牧兽医, 2021, 48(1): 117-123.