关键词: 副猪嗜血杆菌; 分离; 鉴定; 药敏试验

Abstract: The purpose of this study is to determine if there is Glasser’s disease in Heilongjiang,which provide a theory basis for the prevention and control of Glasser’s disease. The dead piglets with polyserositis and arthritis were collected and the bacterium was isolated from the samples. Then the physical and chemical properties of isolated strain were identified,its 16S rRNA gene was amplified by PCR,and this DNA fragment was subsequently cloned and sequenced. The sequenced results were analyzed by BLAST in GenBank. The homology and evolution relations analysis of the similar sequences were done by DNAStar. The drug sensitivity testing was done by isolated strain. The results indicated that a pantomorphic NAD dependent strain was isolated,the results of the physical and chemical properties and PCR showed that was Haemophilus parasuis. The phyletic evolution analysis results of the isolated strain 16S rRNA gene showed that it was in same subgroup with the Haemophilus parasuis in China,Japan and American,however that was in single subgroup in Spain; drug sensitivity testing showed that it was hypersensitive to spectinomycin and cephradine.

Key words: Haemophilus parasuis; isolation; idendification; antibiotic susceptibility test