›› 2010, Vol. 37 ›› Issue (9): 220-222.

• 兽医临床 • 上一篇    下一篇

一例猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染的诊断

徐慧1 ,叶飞1,2,贺云霞1,李方正3,龚玉梅1, 张培君1   

  1. (1.北京市农林科学院畜牧兽医研究所,北京 100097;2.首都师范大学生命科学学院,北京 100037;3青岛农业大学动物科技学院,青岛 266109)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
  • 通讯作者: 张培君

Diagnosis of Mixed Infection of Porcine Circovirus 2,Escherichia coliand Staphylococcus haemolyticus

XU Hui1, YE Fei1,2, HE Yun-xia1,LI Fang-zheng3, GONG Yu-mei1, ZHANG Pei-jun1   

  1. (1.Institute for Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2.College of Life Science, Capital Normal University, Beijing 100037, China; 3.College of Animal Science,Qingdao Agricultural University,Qingdao 266109,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20

摘要: 山东德州某猪场发生猪高热、呼吸系统疾病甚至死亡的疫情。采集病料提取病变组织总DNA或RNA进行猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪细小病毒、猪伪狂犬病病毒、猪瘟病毒的PCR或RT-PCR检测。PCR扩增出353 bp的猪圆环病毒2型特异性条带。同时进行细菌分离培养、生化鉴定等试验,诊断为猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染。

关键词: 猪圆环病毒2型; 大肠杆菌; 溶血葡萄球菌; 混合感染; 诊断

Abstract: A pig farm in Dezhou broke out disease that was similar to swine high fever disease. Samples of diseased pigs were collected. Total DNA and RNA of the samples were extracted. Porcine reproductive and respiratory syndrome virus(PRRSV), porcine circovirus type 2(PCV2), classic swine fever virus(CSFV),pseudorabies virus(PRV) and porcine parvovirus(PPV), were detected by PCR or RT-PCR. A 353 bp band which was specific for porcine circovirus type 2 was amplified. Further tests through bacterial isolation and culture, biochemical identification proved a mixed infection of PCV2, Escherichia coli and Staphylococcus haemolyticus.

Key words: porcine circovirus type 2; Escherichia coli; Staphylococcus haemolyticus; mixed infection; diagnosis

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