›› 2010, Vol. 37 ›› Issue (9): 237-240.

• 经验交流 • 上一篇    

庆大霉素快速Dot-ELISA检测方法的建立及初步应用

陈艳1 ，梁赤周1,2，吴斌3，邹明强1，薛强1，郭良宏2   

  1. （1.中国检验检疫科学研究院， 北京 100123； 2. 中国科学院生态环境研究中心，北京 100085；3. 辽宁出入境检验检疫局技术中心，大连 116001）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
  • 通讯作者: 邹明强

Development and Application of Dot-ELISA for Rapid Detection of Gentamicin Residue in Milk

CHEN Yan1, LIANG Chi-zhou1,2, WU Bin3, ZOU Ming-qiang1, XUE Qiang1, GUO Liang-hong2

  

  1. (1.Chinese Academy of Inspection and Quarantine, Beijing 100123, China; 2.Research Center for Eco-Environmentd Scienses, Chinese Academy of Sciences, Beijing 100085, China; 3. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: ZOU Ming-qiang

摘要： 本研究旨在建立一种适用于快速、现场检测要求的庆大霉素残留Dot-ELISA检测方法。将硝酸纤维素膜用激光切割成小圆片，粘贴到激光打孔的塑料胶条上，制成8个圆片组成的NC膜检测条。通过条件优化，建立了基于NC膜条的庆大霉素残留直接竞争Dot-ELISA检测方法。结果表明，该方法可以快速检测牛奶等食品中的庆大霉素残留，检测灵敏度达到60 ng/mL，可用于庆大霉素残留的筛查。与微孔板ELISA相比，Dot-ELISA可以大大节省检测时间。直接竞争Dot-ELISA具有简便、快速、灵敏、直观的特点，具有推广和应用价值。

关键词: 庆大霉素; Dot-ELISA; NC膜; 快速检测

Abstract: A dot enzyme linked immunosorbent assay (Dot-ELISA) was established for rapid detection of gentamicin residue in milk on-site. The Dot-ELISA was performed on modified nitrocellulose membrane strip (NCM strip) which had eight round pieces of nitrocellulose membrane with diameter of 2 mm, which was cut by Versa Laser, and then was affixed on a plastic adhesive tape. Based on the NCM strip, a direct competitive Dot-ELISA was developed for detecting gentamicin and the influence of several factors on the immunoassay was studied. The optimized Dot-ELISA has been used to detect gentamicin in milk and a limit detection of 60 ng/mL was obtained. Compared with the normal ELISA tests carried on the microtiter plates, the detection time was saved evidently. The Dot-ELISA was characterized by easy use, rapid reaction, economical feasibility, and observable results and the method was worth promoted.

Key words: gentamicin; Dot-ELISA; NCM; rapid detection

中图分类号: 

京ICP备10030713号-19
版权所有 © 2019 中国农业科学院北京畜牧兽医研究所
地址：北京市海淀区圆明园西路农大南路中国农业科学院北京畜牧兽医研究所　邮编：100193　
电话：010-62811226，62810371，62816020　E-mail：zgxmsy@caas.cn
本系统由北京玛格泰克科技发展有限公司设计开发