›› 2010, Vol. 37 ›› Issue (4): 134-137.

• 遗传繁育 • 上一篇    下一篇

ROR基因在绒山羊皮肤中的表达研究

常子丽1,2,张文广1,2,王志新1,张永斌3,高爱琴1,张燕军1,2,丽春1,2,刘兴亮1,2,吴萍1,2,刘维1,2,苏蕊1,2,李金泉1,2   

  1. (1.内蒙古农业大学动物科学与医学学院, 呼和浩特 010018;2.内蒙古自治区动物遗传育种与繁殖重点实验室,呼和浩特 010018;3.内蒙古白绒山羊种羊场, 鄂尔多斯 017000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20
  • 通讯作者: 李金泉

Research on Expression of Retinoid-related Orphan Receptor (ROR) in the Skin of Cashmere Goat

  

  1. (1.College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot 010018,China;2.Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot 010018,China;3.Cashmere Goat Breeding Farm in Inner Mongolia, Ordos 017000,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: LI Jin-quan

摘要: 试验从内蒙古白绒山羊皮肤组织中提取mRNA,经RT-PCR扩增RORα、RORβ、RORγ片段,比较研究ROR基因在内蒙古白绒山羊不同时期1月(退行期)、3月(休止期)、5月(兴盛前期)、9月(兴盛期)份的表达情况。结果发现,RORα、RORβ、RORγ在1、5、9月绒山羊皮肤中表达,在3月份当绒毛生长处于休止期时,该基因的3个亚型均未见表达,且不同的亚型在绒毛生长的不同时期表达规律类同。因此,推测ROR家族的不同亚型均与绒毛的周期性生长有关。

关键词: 褪黑素; 核受体基因; 毛囊

Abstract: To discuss whether the effects of melatonin might result from nuclear receptors, mRNA was extracted from the skin of Inner Mongolia cashmere goat, and retinoid-related orphan receptor α( RORα), retinoid-related orphan receptor β(RORβ) and retinoid-related orphan receptor γ(RORγ) were amplified by RT-PCR, the expression of RORs in the skin of Inner Mongolia cashmere goat from different stages of hair follicle development was explored. The results revealed that the expression of RORα,RORβ and RORγ mRNA was detected in Jan(catagen), May(early anagen) and Sep(anagen),while non-expression in Mar(telogen),and RORα,RORβ and RORγ had the same expression pattern.It suggestted that RORs might have relationship with the cycled growth of cashmere.

Key words: melatonin; nuclear receptors; hair follicle

中图分类号: