构树多糖的提取工艺、结构表征和生物活性研究进展

doi:10.16431/j.cnki.1671-7236.2025.06.019

摘要/Abstract

摘要： 构树(茎叶)中含有丰富的蛋白质，可以作为植物性蛋白原料替代畜禽饲粮中的部分豆粕，目前已经用于多种动物饲料，具有较高的营养价值。2018年，构树(茎叶)被纳入《饲料原料目录》，其饲用价值得到广泛关注；同时，构树也是一种传统的中草药，其果实(楮实子)、叶、根皮等多个部位均可入药。构树具有提高畜禽免疫、改善脂肪代谢、防治鸡球虫感染等药用功效，构树的药用价值正逐步得到研究人员的关注。以往对构树活性成分的研究多集中在黄酮类、生物碱、木脂素等，而对多糖活性的研究较少。构树多糖具有抗氧化、抗微生物、保肝、提高免疫等生物活性，在当前饲料禁抗和养殖减抗的政策背景下，构树多糖或许能够开发为一种新型的功能性饲料，在畜牧领域展现出广阔的应用前景。作者系统综述了构树多糖的提取工艺、结构表征和生物活性，并分析和总结了当前构树多糖的研究热点和不足，以期为构树多糖的进一步开发和在畜禽生产中的应用提供理论参考。

关键词: 构树多糖; 提取工艺; 结构表征; 生物活性

Abstract: Broussonetia papyrifera (stems and leaves) contains abundant protein,which can serve as a plant-based protein source to replace part of the soybean meal in livestock and poultry diets,feeding a variety of animals with high nutritional value.In 2018,the stems and leaves of Broussonetia papyrifera were included in the Catalogue of Feed Raw Materials, drawing widespread attention to its feeding value.Additionally,Broussonetia papyrifera is also a traditional Chinese medicinal herb,with its fruits (Fructus Broussonetiae),leaves,and root bark all having medicinal properties.Broussonetia papyrifera possess medicinal effects such as enhancing the immunity of animals,regulating fat metabolism,and resisting chicken coccidiosis,gradually attracting the attention of researchers for its medicinal value.Previous studies on the active ingredients of Broussonetia papyrifera have mainly focused on flavonoids,alkaloids,lignans,etc.,with less attention paid to the activity of polysaccharides.The polysaccharides from Broussonetia papyrifera have rich biological activities such as antioxidant,antimicrobial,hepatoprotective,and immune enhancing properties.Under the regulations prohibiting the addition of antibiotics in animal feed and reducing the use of antibiotics during feeding,polysaccharides from Broussonetia papyrifera may be developed into a functional feed with broad application prospects in the livestock industry.The author comprehensively reviews the extraction processes,structural characterization and biological activities of polysaccharides from Broussonetia papyrifera,analyzes and summarizes the current research hotspots and deficiencies in this field,aiming to provide theoretical references for further development and utilization of polysaccharides from Broussonetia papyrifera in livestock and poultry production.

Key words: polysaccharides from Broussonetia papyrifera; extraction process; structural characterization; biological activity

中图分类号:

S816.5

徐百昌, 谢宗固, 司红彬. 构树多糖的提取工艺、结构表征和生物活性研究进展[J]. 中国畜牧兽医, 2025, 52(6): 2662-2674.

XU Baichang, XIE Zonggu, SI Hongbin. Research Progress on Extraction Techniques,Structural Characterization and Biological Activities of Polysaccharides Derived from Broussonetia papyrifera[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2662-2674.

参考文献

[1] 张红,陈凤鸣,黄兴国,等.构树叶的营养价值及其在动物生产中的应用研究进展[J].动物营养学报,2020,32(9):4086-4092.ZHANG H,CHEN F M,HUANG X G,et al.Nutritional value of Broussonetia papyrifera leaf and its application in animal production[J].Chinese Journal of Animal Nutrition,2020,32(9):4086-4092.(in Chinese)
[2] PENG X,LIU H,CHEN P,et al.A chromosome-scale genome assembly of paper mulberry (Broussonetia papyrifera) provides new insights into its forage and papermaking usage[J].Molecular Plant,2019,12(5):661-677.
[3] NIU K,WANG Y F,LIANG X,et al.Impact of fermented Broussonetia papyrifera on laying performance,egg quality,lipid metabolism,and follicular development of laying hens[J].Poultry Science,2023,102(5):102569.
[4] WANG R,WANG X,XIONG Y,et al.Dietary paper mulberry silage supplementation improves the growth performance,carcass characteristics,and meat quality of Yangzhou goose[J].Animals,2024,14(3):359.
[5] NIU J,LIU X,XU J,et al.Effects of silage diet on meat quality through Shaping gut microbiota in finishing pigs[J].Microbiology Spectrum, 2023,11(1):e0241622.
[6] 陈光吉,熊先勤,何润霞,等.全株构树青贮在务川黑牛日粮中饲用价值评价[J].中国农业科学,2021,54(19):4218-4228.CHEN G J,XIONG X Q,HE R X,et al.Evaluation of feeding value for whole Broussonetia papyrifera silage in diet of Wuchuan Black beef cattle[J].Scientia Agricultura Sinica,2021,54(19):4218-4228.(in Chinese)
[7] SU Y,CHEN G,CAI Y,et al.Effects of Broussonetia papyrifera-fermented feed on the growth performance and muscle quality of Hu sheep[J].Canadian Journal of Animal Science,2020,100(4):771-780.
[8] TANG T,TONG F,ZHAO S,et al.Effects of fermented Broussonetia papyrifera on growth,intestinal antioxidant,inflammation and microbiota of grass carp (Ctenopharyngodon idella)[J].Aquaculture Reports,2021,20:100673.
[9] 朱开梅,刘建楠,顾生玖,等.构树药用活性化学成分及药理临床应用研究进展[J].中国实验方剂学杂志,2011,17(1):198-201.ZHU K M,LIU J N,GU S J,et al.Progress on chemical constituents,pharmacological effects and clinical applications from Broussonetia papyrifera[J].Chinese Journal of Experimental Traditional Medical Formulae,2011,17(1):198-201.(in Chinese)
[10] 徐百昌,郝凯源,吕菲菲,等.构树叶粉抗鸡柔嫩艾美耳球虫感染的试验研究[J].黑龙江畜牧兽医,2022，2:107-112.XU B C,HAO K Y,LYU F F,et al.Experimental study on the resistance of Broussonetia papyrifera leaf powder against Eimeria tenella infection in chicken[J].Heilongjiang Animal Science and Veterinary Medicine,2022，2:107-112.(in Chinese)
[11] RYU H W,CURTIS-LONG M J,JUNG S,et al.Anticholinesterase potential of flavonols from paper mulberry (Broussonetia papyrifera) and their kinetic studies[J].Food Chemistry,2012,132(3):1244-1250.
[12] HAN Q,WU Z,HUANG B,et al.Extraction,antioxidant and antibacterial activities of Broussonetia papyrifera fruits polysaccharides[J].International Journal of Biological Macromolecules,2016,92:116-124.
[13] TIAN J,LIU T,XUE J,et al.Flavanoids derivatives from the root bark of Broussonetia papyrifera as a tyrosinase inhibitor[J].Industrial Crops and Products,2019,138:111445.
[14] 冯卫生,李红伟,郑晓珂.构树化学成分的研究进展[J].中国新药杂志,2008,17(4):272-278.FENG W S,LI H W,ZHENG X K.Researches of constituents of Broussonetia papyrifera[J].Chinese Journal New Drugs,2008,17(4):272-278.(in Chinese)
[15] GUO M,WANG M,DENG H,et al.A novel anticancer agent broussoflavonol B downregulates estrogen receptor (ER)-α36 expression and inhibits growth of ER-negative breast cancer MDA-MB-231 cells[J]. European journal of Pharmacology,2013,714(1-3):56-64.
[16] ZHENG Z,CHENG K,CHAO J,et al.Tyrosinase inhibitors from paper mulberry (Broussonetia papyrifera)[J].Food Chemistry,2008,106(2):529-535.
[17] 张晓娜,赵曼,丰亚敏,等.响应面法优化构树叶多糖碱提工艺[J].食品工业,2023,44(9):85-88.ZHANG X N,ZHAO M,FENG Y M,et al.Optimization of alkaline extraction conditions of polysaccharides from Broussonetia papyrifera leaves with response surface methodology[J].The Food Industry,2023,44(9):85-88.(in Chinese)
[18] 严丹,蔡延渠,李铁程,等.Box-Behnken响应面法优化航天杂交构树叶多糖提取工艺及体外抗氧化活性研究[J].湖北农业科学,2021,60(3):100-105.YAN D,CAI Y Q,LI T C,et al.Optimization extraction through Box-Behnken response surface methodology and antioxidant activity in vitro of polysaccharides from aerospace hybrid Broussonetia papyrifera leaves[J].Hubei Agricultural Sciences,2021,60(3):100-105.(in Chinese)
[19] 孙杰,毕洁,张初署,等.响应面法提取楮实子多糖研究[J].食品研究与开发,2013,34(6):33-38.SUN J,BI J,ZHANG C S,et al.Study on Broussonetia papyrifera polysaccharides extracting by response surface method[J].Food Research and Development,2013,34(6):33-38.(in Chinese)
[20] 邵金华,唐满生,左海莲.微波法提取楮实子多糖的工艺研究[J].氨基酸和生物资源,2011,33(4):52-54.SHAO J H,TANG M S,ZUO H L.Study on microwave extraction of polysaccharid from Broussonetia papyrifera fruit[J].Amino Acids and Biotic Resources,2011,33(4):52-54.(in Chinese)
[21] 庞怡.构树多糖体外抗猪繁殖与呼吸综合征病毒的活性研究[D].湛江：广东海洋大学,2022.PANG Y.The antivirus activity of polysaccharide from Broussonetia papyrifera against Porcine reproductive and respiratory syndrome virus in vitro[D].Zhanjiang:Guangdong Ocean University,2022.(in Chinese)
[22] XU B,HAO K,CHEN X,et al.Broussonetia papyrifera polysaccharide alleviated acetaminophen-induced liver injury by regulating the intestinal flora[J].Nutrients,2022,14(13):2636.
[23] 舒适,杨娟.响应面分析法优化楮实多糖提取工艺的研究[J].食品工业科技,2009,30(6):218-221.SHU S,YANG J.Optimization of the technology for extracting polysaccharide from Broussonetia papyrifera by response surface methodology (RSM)[J].Science and Technology of Food Industry,2009,30(6):218-221.(in Chinese)
[24] 覃勇荣,余春华,刘旭辉,等.构树果实多糖提取工艺条件的优化[J].山地农业生物学报,2011,30(2):155-160.QIN Y R,YU C H,LIU X H,et al.Optimization of extraction processing for polysaccharile from Broussonetia papyrifera fruit[J].Journal of Mountain Agriculture and Biology,2011,30(2):155-160.(in Chinese)
[25] 祁永华,吴迪,张晏航,等.楮实子多糖提取工艺及抗皮肤光老化能力研究[J].化学工程师,2021,35(7):82-85.QI Y H,WU D,ZHANG Y H,et al.Study on extraction optimization of Fructus broussonetiae polysaccharides and anti skin photoaging[J].Chemical Engineer,2021,35(7):82-85.(in Chinese)
[26] 叶兆伟,王锐丽,刘敏杰,等.构树雄花多糖提取工艺研究[J].江苏农业科学,2012,40(12):292-293.YE Z W,WANG R L,LIU M J,et al.Research on the extraction process of polysaccharides from male flowers of Broussonetia papyrifera[J].Jiangsu Agricultural Sciences,2012,40(12):292-293.(in Chinese)
[27] 孟岩,张玉苍,何连芳,等.构树叶中蛋白和多糖提取工艺研究[J].湖北农业科学,2010,49(4):946-949.MENG Y,ZHANG Y C,HE L F,et al.Study on extracting process of protein and polysaccharide from leaves of Broussonetia papyrifera[J].Hubei Agricultural Sciences,2010,49(4):946-949.(in Chinese)
[28] 孙鸽云.构树叶多种成分的综合提取及黄酮类成分对小鼠表皮干细胞增殖的影响[D].太原：山西医科大学,2014.SUN G Y.Comprehensive extraction of multiple ingredients from Broussonetia papyrifera leaves and the effects on the epidermal stem cells proliferation of the flavonoids[D].Taiyuan:Shanxi Medical University,2014.(in Chinese)
[29] 段雪伟,张敏君,王燕,等.构树花多糖提取工艺优化及其抗氧化活性[J].食品研究与开发,2023,44(21):84-91.DUAN X W,ZHANG M J,WANG Y,et al.Extraction process optimization and antioxidant activity of polysaccharides from Broussonetia papyrifera flowers[J].Food Research and Development,2023,44(21):84-91.(in Chinese)
[30] 谢晓阳,高正龙,王伟,等.杂交构树叶多糖发酵条件优化及抗氧化活性研究[J].饲料研究,2022,45(3):94-100.XIE X Y,GAO Z L,WANG W,et al.Study on antioxidant activity fermentation technology of polysaccharides from hybrid Broussonetia papyrifera leaf[J].Feed Research,2022,45(3):94-100.(in Chinese)
[31] CHEN R,ZHOU X,DENG Q,et al.Extraction,structural characterization and biological activities of polysaccharides from mulberry leaves:A review[J].International Journal of Biological Macromolecules,2024,257(2):128669.
[32] 王文君,侯志华,任媛媛,等.不同构树品种药用化学成分含量比较和综合评价[J].河南林业科技,2020,40(4):1-5.WANG W J,HOU Z H,REN Y Y,et al.Comparison and comprehensive evaluation of nedicinal components in 4Broussonetia papyrifera cultivar[J].Journal of Henan Forestry Science and Technology,2020,40(4):1-5.(in Chinese)
[33] 左鑫.构树枝叶粉对鹅饲用价值的研究[D].广州:华南农业大学,2020.ZUO X.Study on the feeding values of Broussonetia papyrifera twig leaves in geese diets[D].Guangzhou:South China Agricultural University,2020.(in Chinese)
[34] 翟晓巧,赵琳,王文君,等.不同年龄构树根中5种化学成分变化研究[J].河南林业科技,2018,38(1):1-3.ZHAI X Q,ZHAO L,WANG W J,et al.Study on 5 chemical composition change in the root of Broussonetia papyrifera of different ages[J].Journal of Henan Forestry Science and Technology,2018,38(1):1-3.(in Chinese)
[35] 张晓娜,赵曼,郭鹏辉.响应面法优化构树叶多糖酶法提取工艺的研究[J].农产品加工,2024，15:41-43.ZHANG X N,ZHAO M,GUO P H.Optimization of the enzyme extraction process of Broussonetia papyrifera leaf by response surface methodology[J].Farm Products Processing,2024,15:41-43.(in Chinese)
[36] 石慧芳.三种饲用木本植物营养价值及次生代谢物质活性研究[D].贵阳:贵州大学,2021.SHI H F.Studies on the nutritional value and secondary metabolite activity of three woody forage[D].Guiyang:Guizhou University,2021.(in Chinese)
[37] 余伟.构树叶多糖对摄入氧化鱼油大鼠肠道和肝脏健康的影响[D].成都:四川农业大学,2023.YU W.Effects of polyccharides from Broussonetia papyrifera leaves on intestinal and liver health in rats fed the diet containing oxidized fish oil[D].Chengdu:Sichuan Agricultural University,2023.(in Chinese)
[38] AI J,BAO B,BATTINO M,et al.Recent advances on bioactive polysaccharides from mulberry[J].Food & Function,2021,12(12):5219-5235.
[39] XUE H,LI P,BIAN J,et al.Extraction,purification,structure,modification,and biological activity of traditional Chinese medicine polysaccharides:A review[J].Frontiers in Nutrition,2022,9:1005181.
[40] 舒适.楮实和淫羊藿多糖的分离纯化、理化性质及活性研究[D].贵阳:贵州大学,2011.SHU S.Isolation,purification,physical and chemical properties,and activity study of polysaccharides from Fructus broussonetiae and Epimedium brevicornu Maxim[D].Guiyang:Guizhou University,2011.(in Chinese)
[41] ZHANG J,LI C,HUANG Q,et al.Comparative study on the physicochemical properties and bioactivities of polysaccharide fractions extracted from Fructus mori at different temperatures[J].Food & Function,2019,10(1):410-421.
[42] LIANG X,LIU M,WEI Y,et al.Structural characteristics and structure-activity relationship of four polysaccharides from Lycii fructus[J].International Journal of Biological Macromolecules,2023,253(5):127256.
[43] LING N,TIAN H,WANG Q,et al.Advance in Hippophae rhamnoides polysaccharides:Extraction,structural characteristics,pharmacological activity,structure-activity relationship and application[J].International Journal of Biological Macromolecules,2024,270(2):132420.
[44] FERNANDES P A R,COIMBRA M A.The antioxidant activity of polysaccharides:A structure-function relationship overview[J].Carbohydrate Polymers,2023,314:120965.
[45] CHEN N,JIANG T,XU J,et al.The relationship between polysaccharide structure and its antioxidant activity needs to be systematically elucidated[J].International Journal of Biological Macromolecules,2024,270(2):132391.
[46] 赖梓漩,宋雨轩,段雪伟,等.构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用及机制研究[J].日用化学工业(中英文),2024,54(9):1069-1077.LAI Z X,SONG Y X,DUAN X W,et al.The protective effect and mechanism of crude polysaccharide from Broussonetia papyrifera flower on UV-induced skin photodamage[J].China Surfactant Detergent & Cosmetics,2024,54(9):1069-1077.(in Chinese)
[47] 张兴芳.楮实子防治药物性肝损伤的作用及氧化应激机制[D].保定:河北中医学院,2020.ZHANG X F.Prevention and treatment of Fructus Broussonetiae on drug-induced liver injury and oxidative stress mechanism[D].Baoding:Hebei University of Chinese Medicine,2020.(in Chinese)
[48] 韩世诚,王婷婷,张一昕,等.楮实子预处理对对乙酰氨基酚致L02细胞损伤的保护作用[J].中国医药导报,2024,21(1):34-38.HAN S C,WANG T T,ZHANG Y X,et al.Protective effect of Fructus broussonetiae on APAP-induced injury of L02 cells[J].China Medical Herald,2024,21(1):34-38.(in Chinese)
[49] 林森柱,史心茹,孙筱梦,等.构树多糖对黄曲霉毒素B₁致雏鸡肝损伤的缓解作用[J].现代牧业,2023,7(4):26-31.LIN S Z,SHI X R,SUN X M,et al.The mitigating effect of Broussonetia papyrifera polysaccharide on aflatoxin B₁-induced hepatic injury in chicken[J].Modern Animal Husbandry,2023,7(4):26-31.(in Chinese)
[50] 茹盟.构树叶提取物对DSS诱导的小鼠结肠炎的缓解作用[D].南昌:江西农业大学,2023.RU M.Protective effect of Broussonetia papyrifera leaf extracton DSS-induced colitis in mice[D].Nanchang:Jiangxi Agricultural University,2023.(in Chinese)
[51] FAN X,YU W,WANG Q,et al.Protective effect of Broussonetia papyrifera leaf polysaccharides on intestinal integrity in a rat model of diet-induced oxidative stress[J].International Journal of Biological Macromolecules,2024,268(1):131589.
[52] YAN W,LUO J,YU Z,et al.A critical review on intestinal mucosal barrier protection effects of dietary polysaccharides[J].Food & Function,2024,15(2):481-492.
[53] XIONG Y,WANG X,LI X,et al.Exploring the rumen microbiota of Hu lambs in response to diet with Paper Mulberry[J].Applied Microbiology and Biotechnology,2023,107(15):4961-4971.
[54] LIANG X,RU M,ZHAI Z,et al.In vitro antibacterial effects of Broussonetia papyrifera leaf extract and its anti-colitis in DSS-treated mice[J].Frontiers in Cellular and Infection Microbiology,2023,13:1255127.
[55] ZHOU Y,CHEN X,CHEN T,et al.A review of the antibacterial activity and mechanisms of plant polysaccharides[J].Trends in Food Science & Technology,2022,123:264-280.
[56] QIAO Y,SHEN Y,JIANG H,et al.Structural characterization,antioxidant and antibacterial activity of three pectin polysaccharides from blueberry[J].International Journal of Biological Macromolecules,2024,262(1):129707.
[57] LU W,YANG Z,CHEN J,et al.Recent advances in antiviral activities and potential mechanisms of sulfated polysaccharides[J].Carbohydrate Polymers,2021,272:118526.
[58] ZHAO R,YANG F,BAI Y,et al.Research progress on the mechanisms underlying poultry immune regulation by plant polysaccharides[J].Frontiers in Veterinary Science,2023,10:1175848.
[59] XIE Z,JIANG N,LIN M,et al.The Mechanisms of polysaccharides from tonic Chinese herbal medicine on the enhancement immune function:A review[J].Molecules,2023,28(21):7355.
[60] 徐光沛,李涵涛,邓奇元,等.环磷酰胺对小鼠血常规、血液生化指标的影响及构树叶多糖的干预作用[J].安徽科技学院学报,2023,37(3):76-82.XU G P,LI H T,DENG Q Y,et al.Effects of cyclophosphamide on routine blood and blood biochemical indices in mice and interventional effects of Broussonetia papyrifera polysaccharides[J].Journal of Anhui Science and Technology University,2023,37(3):76-82.(in Chinese)
[61] 徐光沛，蒋平,何燕飞,等.构树叶粗多糖对环磷酰胺诱导免疫抑制小鼠免疫功能的影响[J].西北农业学报,2022,31(7):815-822.XU G P,JIANG P,HE Y F,et al.Effect of crude polysaccharide of Broussonetia papyrifera leaf on immune function in cyclophosphamide induced mice[J].Acta Agriculturae Boreali-Occidentalis Sinica,2022,31(7):815-822.(in Chinese)
[62] 程新星,孙黔云,杨娟,等.九种黔产植物多糖的体外免疫调节活性研究[J].贵州师范大学学报(自然科学版),2012,30(4):5-8.CHENG X X,SUN Q Y,YANG J,et al. In vitro screening of immunomodulatory activity of polysaccharides of nine species of plants in Guizhou province[J].Journal of Guizhou Normal University (Natural Sciences),2012,30(4):5-8.(in Chinese)
[63] BUSCHE M A,HYMAN B T.Synergy between amyloid-β and tau in Alzheimer’s disease[J].Nature Neuroscience,2020,23(10):1183-1193.
[64] 张二飞,殷紫,闫宇辉,等.楮实子对APP/PS1双转基因小鼠海马神经发生的影响[J].中成药,2019,41(3):539-543.ZHANG E F,YIN Z,YAN Y H,et al.Effects of Broussonetiae fructus on hippocampal neurogenesis in double transgenic APP/PS1 mice[J].Chinese Traditional Patent Medicine,2019,41(3):539-543.(in Chinese)
[65] 孙红婷,宋艳荣.楮实子提取物对APP/PS1小鼠学习记忆能力及对PI3K-CREB-XBP1信号通路的影响[J].中国免疫学杂志,2019,35(24):3021-3026.SUN H T,SONG Y R.Effect of extract of Fructus broussonetiae on learning and memory impairment in APP/PS1 mice and its effect on PI3K-CREB-XBP1 signaling pathway[J].Chinese Journal of Immunology,2019,35(24):3021-3026.(in Chinese)
[66] YAN Y,HUANG Z,XIONG Q,et al.Effects of Broussonetia papyrifera (L.) L’Hér.ex Vent.fruits water extract on hippocampal neurogenesis in the treatment of APP/PS1 transgenic mice[J].Frontiers in Pharmacology,2022,13:1056614.
[67] 姜倩倩,梁少瑜,李新萍,等.楮实子多糖的制备、分离及其对Aβ_25-35诱导的SH-SY5Y细胞损伤和AChE的抑制作用[J].深圳中西医结合杂志,2021,31(17):1-4.JIANG Q Q,LIANG S Y,LI X P,et al.Preparation and isolation of Fructus broussonetiae polysaccharide and its inhibitory effect on AChE and SH-SY5Y cell damage induced by Aβ_25-35[J].Shenzhen Journal of Integrated Traditional Chinese and Western Medicine,2021,31(17):1-4.(in Chinese)
[68] 王丽敏,舒适,杨娟.楮实多糖对小鼠抗疲劳作用的研究[J].食品研究与开发,2019,40(22):25-28.WANG L M,SHU S,YANG J.Study on antifatigue effect of Broussonetia papyrifera fruit polysaccharide in mice[J]. Food Research and Development,2019,40(22):25-28.(in Chinese)
[69] 黄冰,谭大雁,毛湘冰,等.灌服构树叶多糖对氧化鱼油饲粮诱导的大鼠肝脏功能损伤的影响[J].动物营养学报,2023,35(12):8074-8082.HUANG B,TAN D Y,MAO X B,et al.Effects of oral administration of Broussonetia papyrifera leaf polysaccharides on liver dysfunction of rats fed diet containing oxidized fish oil[J].Chinese Journal of Animal Nutrition,2023,35(12):8074-8082.(in Chinese)
[70] 彭梅,张振东,杨娟.14种多糖对小鼠S₁₈₀肉瘤抑制活性筛选[J].山地农业生物学报,2011,30(1):56-59.PENG M,ZHANG Z D,YANG J.Inhibitory effects of 14 polysaccharides on sarcocarcinoma S₁₈₀ of mice[J].Journal of Mountain Agriculture and Biology,2011,30(1):56-59.(in Chinese)
[71] 王皓南,范晶,余坤子,等.霍山石斛多糖的结构分析、生物活性研究进展及与不同种间多糖成分差异性分析[J].中草药,2023,54(15):5044-5056.WANG H N,FAN J,YU K Z,et al.Progress on structural analysis and bioactivity of polysaccharides of Dendrobium huoshanense and analysis of differences in polysaccharide composition between different species[J].Chinese Traditional and Herbal Drugs,2023,54(15):5044-5056.(in Chinese)
[72] MENG Y,LYU F,XU X,et al.Recent advances in chain conformation and bioactivities of triple-helix polysaccharides[J].Biomacromolecules,2020,21(5):1653-1677.
[73] DU B,YANG Y,BIAN Z,et al.Molecular weight and helix conformation determine intestinal anti-inflammatory effects of exopolysaccharide from Schizophyllum commune[J].Carbohydrate Polymers,2017,172:68-77.
[74] SONG Q,WANG Y,HUANG L,et al.Review of the relationships among polysaccharides,gut microbiota,and human health[J].Food Research International,2021,140:109858.
[75] QU J,HUANG P,ZHANG L,et al.Hepatoprotective effect of plant polysaccharides from natural resources:A review of the mechanisms and structure-activity relationship[J].International Journal of Biological Macromolecules,2020,161:24-34.