lncRNA对骨骼肌卫星细胞发育的调控研究进展

doi:10.16431/j.cnki.1671-7236.2025.05.023

摘要/Abstract

摘要： 骨骼肌是畜禽的主要产品、人类重要的肉食品和蛋白质来源，也是养殖业重要的经济性状。位于肌膜和基膜之间的骨骼肌卫星细胞(SCs)是骨骼肌的干细胞，其增殖与分化的调控在动物出生后机体骨骼肌发育、维持和损伤后再生修复等生命活动中都发挥着至关重要的作用，与动物肌肉产量和品质密切相关。长链非编码RNA(lncRNA)是广泛存在于真核细胞的细胞核、细胞器、细胞质和细胞膜内的一类长度＞200 nt的具有较低或无编码能力的RNA分子，通过作为竞争性内源RNA(ceRNA)，发挥靶微小RNA(miRNA)分子吸附海绵作用，参与基因表达调控、表观遗传调控、细胞周期与分化调控及器官发育等多种生物学过程。近年来，随着转录组学、蛋白质组学、代谢组学等技术的发展，不断有新的与调控牛、羊、猪、猴、鸡及鼠等动物骨骼肌发育有关的lncRNA被发现和报道。lncRNA具有调控SCs的激活、增殖、分化及脂质代谢等肌肉发育的作用。作者对骨骼肌SCs增殖、分化过程中关键lncRNA、上下游靶点、信号通路及作用机制进行了综述，并对lncRNA调控骨骼肌SCs的增殖、分化的分子机制和技术研究提出了展望，旨在为畜禽肌肉发育、产肉性能和肉品质改良提供一定的理论参考。

关键词: 骨骼肌卫星细胞; 长链非编码RNA; 成肌分化; 增殖; 调控因子

Abstract: Skeletal muscle is the main product of livestock and poultry,an important source of meat and protein for human beings,and also an important economic character of aquaculture.Skeletal muscle satellite cells (SCs)，located between the sarcolemma and basement membrane,are the stem cells of skeletal muscle,the regulation on proliferation and differentiation plays an important role in the development,maintenance,regeneration and restoration of skeletal muscle after birth,and are closely related to muscle production and quality.Long non-coding RNA (lncRNA) is non-coding RNA molecule with a length of more than 200 nucleotides and low or no protein-coding potential,which exists widely in the nucleus,organelles,cytoplasm and membrane of eukaryotic cells.By acting as competitive endogenous RNA (ceRNA),lncRNA serves as a sponge for target microRNA (miRNA),is participating in various biological processes such as gene expression regulation,epigenetic regulation,cell cycle and differentiation regulation,and organ development.With the development of transcriptomics,proteomics and metabolomics,new lncRNA involves in the regulation of skeletal muscle development in cattle,sheep,pigs,monkeys,chickens and mice have been discovered and reported.lncRNA regulates the activation,proliferation,differentiation and lipid metabolism of SCs.The authors review the key lncRNA,upstream and downstream targets,signaling pathway and mechanism in the proliferation and differentiation of skeletal muscle SCs,and forward prospect for the molecular mechanism and technical research of lncRNA regulating skeletal muscle SCs,in order to provide theoretical references for the muscle development,meat production performance and improvement of meat quality in livestock and poultry.

Key words: skeletal muscle satellite cells; long non-coding RNA; myogenic differentiation; proliferation; regulatory factor

中图分类号:

S852.23

李健, 魏亚楠, 李法磊, 张慧琳, 李东伟, 刘勇, 徐高骁. lncRNA对骨骼肌卫星细胞发育的调控研究进展[J]. 中国畜牧兽医, 2025, 52(5): 2187-2197.

LI Jian, WEI Yanan, LI Falei, ZHANG Huilin, LI Dongwei, LIU Yong, XU Gaoxiao. Research Progress on the Regulation of lncRNA on the Development of Skeletal Muscle Satellite Cells[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2187-2197.

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