不同方法检测鸡白痢沙门氏菌抗体消长规律的比较研究

doi:10.16431/j.cnki.1671-7236.2018.02.026

摘要/Abstract

摘要：

为比较不同方法检测鸡白痢沙门氏菌抗体消长的规律，以便为种鸡场净化提供科学指导，本研究以鸡白痢沙门氏菌活菌和灭活免疫原分别接种SPF鸡，采用平板凝集、微量凝集和ELISA试验定期检测血清中的特异性抗体，并以Kappa检验判定不同检测方法之间的一致性程度。结果显示，平板凝集试验在接种后检出抗体阳转的时间早于ELISA，但ELISA检出抗体阳性的持续时间更长，且更符合抗体消长规律；3种检测方法的结果之间仅具有微弱一致性（Kappa系数为0.002~0.295）。本研究结果表明，不同鸡白痢沙门氏菌抗体检测方法之间存在较大差异，但从总体来看，ELISA无假阳性干扰，检出抗体的持续时间较长，更符合抗体消长规律，在单一鸡白痢沙门氏菌感染的情况下，更有利于种鸡场对该病进行净化。

关键词: 鸡白痢沙门氏菌; 人工感染; 抗体; 平板凝集试验; 微量凝集试验; ELISA; Kappa检验

Abstract:

To compare and evaluate the different methods for detection Salmonella Pullorum (S. Pullorum) antibody, and to provide scientific guidance for the purification of breeding chicken farms, SPF chickens were inoculated with active and inactivated S. Pullorum. Serum samples were collected and detected by three different methods (rapid serum agglutination, micro-agglutination and ELISA), and Kappa test was applied to determine the concordance among different methods. The results showed that positive samples were detected earlier when using rapid serum agglutination than ELISA, but the later method had a longer detection period above the threshold on positive samples, which was more consistent with the dynamic rule of antibody in S. Pullorum positive chickens. It showed weak agreement among three different methods (Kappa value were 0.002 to 0.295). In conclusion, the study demonstrated that there were big differences among three methods for S. Pullorum antibody detection. ELISA might be the best choice in single S. Pullorum infection, because of its no false positive interference, longer detection period above the threshold on S. Pullorum positive samples and more consistent with the dynamic rule of antibody.

Key words: Salmonella Pullorum; artificial infection; antibody; rapid serum agglutination test; micro-agglutination test; ELISA; Kappa test

中图分类号:

S858.31

刘洋, 李丹, 王传彬, 霍斯琪, 刘玉良, 顾小雪. 不同方法检测鸡白痢沙门氏菌抗体消长规律的比较研究[J]. 《中国畜牧兽医》, 2018, 45(2): 493-500.

LIU Yang, LI Dan, WANG Chuanbin, HUO Siqi, LIU Yuliang, GU Xiaoxue. A Comparative Study of Various Methods for the Detection of Dynamic Rule of the Antibody Against Salmonella Pullorum[J]. , 2018, 45(2): 493-500.

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