丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响

中国畜牧兽医

丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响

谢炳坤¹，吕玲燕²，陆杏蓉²，崔奎青²，孙俊铭²，覃兆鲜¹，石德顺²，刘庆友²

（1.广西壮族自治区畜牧研究所，广西南宁 530005；2.广西大学亚热带生物资源保护利用国家重点实验室，广西南宁 530005）

收稿日期:2013-10-30 出版日期:2014-05-20 发布日期:2014-06-25
通讯作者: 石德顺。E-mail：ardsshi@gxu.edu.cn 刘庆友。E-mail：qyliu2002@126.com
作者简介:谢炳坤（1979—），男，广西人，博士，副研究员，研究方向：动物胚胎工程。
基金资助:
国家自然科学基金（30960251）；桂科基金（2010GXNSFA013097）；广西家畜遗传改良重点实验室开放基金（2013GXKFKT-08）。

Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos

XIE Bing-kun¹, LV Ling-yan², LU Xing-rong²，CUI Kui-qing², SUN Jun-ming², QIN Zhao-xian¹,SHI De-shun², LIU Qing-you²

（1.Guangxi Zhuang Autonomous Region Animal Husbandry Institute，Nanning 530005，China；2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University，Nanning 530005，China）

Received:2013-10-30 Online:2014-05-20 Published:2014-06-25

摘要/Abstract

摘要： 为探讨组蛋白去乙酰化酶抑制剂丙戊酸（valproic acid, VPA)对猪手工克隆(HMC)胚胎发育潜能的影响，本研究比较了不同浓度（0、25、50、75和100 nmol/L）VPA处理猪HMC重构胚对后期胚胎发育率、内细胞团数和组蛋白乙酰化程度的影响。结果表明，50、75 nmol/L VPA处理组HMC重构胚的卵裂率和囊胚发育率均显著高于0、25和100 nmol/L VPA处理组（P<0.05）；与0、25、75和100 nmol/L相比，50 nmol/L VPA处理组能显著提高囊胚内细胞团细胞数（P<0.05），囊胚阶段VPA处理组的组蛋白H3K14乙酰化（AcH3K14）水平高于空白组和孤雌激活囊胚。因此，应用VPA处理可提高猪HMC重构胚胎分裂率、囊胚率及增加内细胞团细胞数，提高了猪HMC胚胎的体外发育潜能。

关键词:

猪; 手工克隆; 丙戊酸; 发育潜能

Abstract: To explore the effects of histone deaceylase inhibitors valproic acid (VPA) affecting the development potency of handmade cloned (HMC) embryos, porcine HMC reconstructed embryos were cultured for 24 h with different concentration (0，25，50，75 and 100 nmol/L) of VPA, and the blastocyst development rate, inner cell mass (ICM) cells number and histone acetylation level were compared. The results showed that reconstructed embryos were cultured in PZM-3 containing 50， 75 nmol/L VPA for 24 h, the frequency of the rate of cleavage and blastocyst formation were significantly higher in embryos treated with VPA than 0, 25, 100 nmol/L VPA treatments （P＜0.05）. In addition, 50 nmol/L VPA treatment significantly increased the number of inner cell mass (ICM) cells compared with the other groups (P＜0.05). The AcH3K14 level in embryos treated with VPA was higher than the control group and parthenogenetic activation group. In conclusion, epigenetic modification VPA could enhances the rate of cleavage, blastocyst formation and the ICM cells number of porcine HMC reconstructed embryos, which mean improve the developmental potency of HMC embryos.

Key words: porcine; handmade cloning; valproic acid (VPA); developmental potency

谢炳坤，吕玲燕，陆杏蓉，崔奎青，孙俊铭，覃兆鲜，石德顺，刘庆友. 丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响[J]. 中国畜牧兽医.

XIE Bing-kun, LV Ling-yan, LU Xing-rong，CUI Kui-qing, SUN Jun-ming, QIN Zhao-xian,SHI De-shun, LIU Qing-you. Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos[J]. .

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