不同源鼠伤寒沙门氏菌耐药性质粒不相容性分群的初步研究

中国畜牧兽医

不同源鼠伤寒沙门氏菌耐药性质粒不相容性分群的初步研究

李静怡，崔可琦，冯赛祥，贺现辉，廖明，徐成刚

（华南农业大学兽医学院，农业部兽用疫苗创制重点实验室，广东广州 510642）

收稿日期:2013-09-06 出版日期:2014-03-20 发布日期:2014-05-15
通讯作者: 徐成刚。E-mail:chgangxu@126.com
作者简介:李静怡（1989—），女，广东人，硕士生，研究方向：细菌分子生物学。
基金资助:
广东省科技计划项目(2009B030803050、2011B020306010)。

Preliminary Study on Incompatibility Plasmids of 101 Strains ofSalmonella typhimurium by PCR-based Replicon Typing

LI Jing-yi,CUI Ke-qi,FENG Sai-xiang,HE Xian-hui,LIAO Ming,XU Cheng-gang

(Key Laboratory of Veterinary Vaccine Innovation, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:2013-09-06 Online:2014-03-20 Published:2014-05-15

摘要/Abstract

摘要： 试验利用PCR方法检测101株不同源鼠伤寒沙门氏菌携带质粒的不相容群，共设计18对引物，PCR分为5个三重PCR和3个单一PCR，用来检测识别FIA、FIB、FIC、HI1、HI2、I1、L/M、N、P、W、T、A/C、K、B/O、X、Y、F 和FII复制子。101株不同源鼠伤寒沙门氏菌中94株（93.1%）携带质粒不相容群，共携带10种质粒不相容群，分别是Inc HI1、Inc HI2、Inc I1、Inc FIA、Inc FIB、Inc N、Inc Y、Inc P、Inc FIIA和Inc FrepB，其中76.6%的菌株携带2个或2个以上质粒不相容群。结合药物敏感性试验结果，追踪鼠伤寒沙门氏菌耐药传播机制。本研究调查了耐药性质粒在鼠伤寒沙门氏菌的水平传播特性，不同环境中大量的菌株介导特定多重耐药性质粒的扩散，对调查跟踪质粒赋予耐药的流行病学有重要意义。

关键词: 鼠伤寒沙门氏菌; 质粒分型; 质粒不相容群

Abstract: The incompatibility plasmids of 101 strains Salmonella typhimurium were detected by PCR-based replicon typing. Eighteen pairs of primers were designed to perform 5 triplex-PCRs and 3 simplex-PCRs, recognizing FIA, FIB, FIC, HI1, HI2, I1, L/M, N, P, W, T, A/C, K, B/O, X, Y, F and FII. 94 strains (93.1%) Salmonella typhimurium carried a total of 10 Inc groups. The results suggested that the method was applicable for a large number of strains to trace the diffusion of specific multi-drug resistance plasmids in different environments.

Key words: Salmonella typhimurium; plasmid typing; incompatibility plasmid

李静怡，崔可琦，冯赛祥，贺现辉，廖明，徐成刚. 不同源鼠伤寒沙门氏菌耐药性质粒不相容性分群的初步研究[J]. 中国畜牧兽医.

LI Jing-yi,CUI Ke-qi,FENG Sai-xiang,HE Xian-hui,LIAO Ming,XU Cheng-gang. Preliminary Study on Incompatibility Plasmids of 101 Strains ofSalmonella typhimurium by PCR-based Replicon Typing[J]. .

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