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正交设计优化复方淫羊藿的提取工艺研究

谷宇锋1,黄亚军1,王琴1,伍勇1,刘兆颖1,张明军1,孙志良2,蒋政云3   

  1.  (1.湖南农业大学动物医学院,湖南长沙 410128;2.湖南省兽药工程技术研究中心,湖南长沙 410128;3.湖南农业大学动物保健工程技术研究中心,湖南长沙 410128)
  • 收稿日期:2013-07-11 出版日期:2014-03-20 发布日期:2014-05-15
  • 通讯作者: 伍勇(1981—),男,湖南人,讲师,从事中药有效成份与药物代谢研究。E-mail:wuyong712@126.com;Tel:0731-84635054
  • 作者简介:谷宇锋(1989—),男,湖南人,学士,研究方向:中药有效成份与药物代谢。
  • 基金资助:

    湖南农业大学大学生科技创性基金;湖南农业大学大学生创新性实验计划项目(XCX12093)。

Study on the Extraction Process of Compound Epimedium by Orthogonal Experimental Design

GU Yu-feng1, HUANG Ya-jun1, WANG Qin1, WU Yong1, LIU Zhao-ying1, ZHANG Ming-jun1, SUN Zhi-liang2, JIANG Zheng-yun3   

  1. (1.Veterinary Facultry, Hunan Agricultural University, Changsha 410128, China;2.Hunan Engineering Research Center of Veterinary Drug, Changsha 410128,China;3.Animal Health Engineering and Technology Research Center, Hunan Agricultural University, Changsha 410128,China)
  • Received:2013-07-11 Online:2014-03-20 Published:2014-05-15

摘要: 为了优选复方淫羊藿的最佳提取工艺条件,本试验采用正交试验法,以药材加水量、提取次数和提取时间为自变量,以君药淫羊藿的指标成分淫羊藿苷的提取率为评价指标,进行数理统计选取最佳提取工艺。结果显示最佳提取工艺为加10倍药材质量的水,提取3次,每次提取1.5 h。结果表明,该工艺为提高淫羊藿苷的提取率提供了试验基础,并为进一步的工业化生产提供科学理论依据。

关键词: 复方淫羊藿; 淫羊藿苷; 正交设计; 提取工艺

Abstract: The assay was aimed to optimize the extraction process of the compound Epimedium by orthogonal experimental design. The extraction process of the compound Epimedium was optimized with water volume, extraction times and extraction time as independent variables. The extraction ratio of icariin was choosed as an evaluation index. The optimized extraction conditions were as follows, extracted in 10 times water (solid-liquid rate at 1∶10) for 3 times and heated for 1.5 h per time. These results indicated that the optimum extraction technology by the orthogonal experimental design provided experimental bases for improving the content of icariin and its industrial production.

Key words: compound Epimedium; icariin; orthogonal experimental design; extraction process