牡蛎粗多糖对免疫应激大鼠PPARγ mRNA 表达水平的影响

›› 2014, Vol. 41 ›› Issue (12): 40-44.

牡蛎粗多糖对免疫应激大鼠PPARγ mRNA 表达水平的影响

许翔宇, 张儒雅, 岳刚, 黄志坚

福建农林大学动物科学学院, 福建省动物药物工程实验室, 福建福州 350002

收稿日期:2014-07-14 出版日期:2014-12-20 发布日期:2014-12-25
通讯作者: 黄志坚 E-mail:huangzj1999@sina.com
作者简介:许翔宇(1993－),男,浙江人,学士,研究方向:动物医学.
基金资助:
国家级"大学生创新创业训练计划"项目(201310389019).

Effects of Oyster Crude Polysaccharides on Rats PPARγ mRNA Expression after Immunological Stress

XU Xiang-yu, ZHANG Ru-ya, YUE Gang, HUANG Zhi-jian

Engineering Laboratory of Animal Pharmaceuticals of Fujian Province, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2014-07-14 Online:2014-12-20 Published:2014-12-25

摘要/Abstract

摘要： 本试验旨在研究牡蛎粗多糖对脂多糖(LPS)刺激大白鼠产生的PPARγ mRNA表达水平的影响.试验将30只大白鼠随机分为空白对照组、应激对照组及牡蛎粗多糖低、中、高剂量组.其空白对照组及应激对照组饲喂基础日粮,牡蛎粗多糖低、中、高剂量组为基础日粮分别添加0.3%、0.6%、0.9%的牡蛎粗多糖.试验期28 d,试验最后1 d,应激对照组、牡蛎粗多糖低、中、高剂量组腹腔注射100 μg/kg LPS,空白对照组注射等量的生理盐水,2 h后剖检,并利用荧光定量PCR法研究牡蛎粗多糖对免疫应激大鼠PPARγ mRNA相对表达量的变化.结果显示,牡蛎粗多糖高、中、低剂量组和空白对照组的肝脏、脾脏和淋巴结PPARγ mRNA的相对表达量均极显著低于应激对照组(P<0.01),但在肾上腺中空白对照组PPARγ mRNA相对表达量与应激对照组相比显著降低(P<0.05).在肾上腺和肝脏中,牡蛎粗多糖高、中剂量PPARγ mRNA相对表达量无显著差异(P>0.05),在脾脏中二者差异显著(P<0.05),淋巴结中二者差异极显著(P<0.01).结果表明添加牡蛎粗多糖的基础日粮可以缓解LPS所引起的大白鼠免疫应激,且添加量为0.3%～0.6%时效果较好.

关键词: 牡蛎粗多糖; 免疫应激; PPARγ mRNA

Abstract: This experiment was aimed to study whether oyster crude polysaccharides could relieve the immune stress stimulated by LPS.Test randomly divided 30 rats into blank control group, stress control group, oyster crude polysaccharides low, medium and high dose groups.Blank control group and stress control group were fed with based diet, oyster crude polysaccharides low, medium and high dose groups were fed with based diet with 0.3%, 0.6% and 0.9% oyster crude polysaccharides.Test lasted for 28 d, the stress control group and oyster crude polysaccharides low, medium and high dose groups were intraperitoneal injected with 100 μg/kg LPS, blank control group with the same amount of saline injection, 2 h later, autopsied the rats and used relative fluorescence quantitative PCR to study the changes of the relative expression quantity of PPARγ mRNA which oyster crude polysaccharides acted on the immune stress rats.The results showed that in oyster crude polysaccharides high, medium, low dose groups and blank control group, the relative expression quantities of PPARγ mRNA in liver, spleen and lymph nodes were extremely significantly lower than that of stress control group (P<0.01), but in adrenal gland, the relative expression quantities of PPARγ mRNA of blank control group significantly decreased compared with stress control group (P<0.05).In adrenal gland and liver, there was no significant difference between oyster crude polysaccharides high and medium dose groups(P>0.05);but in spleen, there was significantly different between those two groups(P<0.05);in lymph nodes, there was extremely significantly different between those two groups(P<0.01).Adding oyster crude polysaccharides into the based diet could relieve the immunological stress from LPS, and the ease effect was better when adding amount was within the range of 0.3% to 0.6%.

Key words: oyster crude polysaccharides; immunological stress; PPARγ mRNA

中图分类号:

Q786

许翔宇, 张儒雅, 岳刚, 黄志坚. 牡蛎粗多糖对免疫应激大鼠PPARγ mRNA 表达水平的影响[J]. , 2014, 41(12): 40-44.

XU Xiang-yu, ZHANG Ru-ya, YUE Gang, HUANG Zhi-jian. Effects of Oyster Crude Polysaccharides on Rats PPARγ mRNA Expression after Immunological Stress[J]. , 2014, 41(12): 40-44.

参考文献

1 王亚东. PPARγ激动剂罗格列酮对体外脂多糖刺激的枯否细胞中IκB激酶β表达影响的研究[D].石家庄:河北医科大学,2007.
2 王俊,姚滢,张建鹏,等. 牡蛎多糖的制备和生物学活性研究[J]. 医学研究生学报,2006,3:217～220.
3 王静,徐萍,侯彦强,等. PPARγ激动剂对单核细胞炎症反应的调控作用[J]. 上海交通大学学报(医学版),2012,7:861～865.
4 尹庆宁,孙学权. 给仔猪注射猪瘟和口蹄疫疫苗产生不良反应的报告[J]. 当代畜禽养殖业,2005,3:9.
5 石君霞. 脂多糖刺激对断奶仔猪PPARγ表达的影响[D].武汉:武汉工业学院,2008.
6 石君霞,刘玉兰,鲁晶,等. 脂多糖对断奶仔猪外周血免疫细胞和免疫器官中PPARγ mRNA表达水平的影响[J]. 畜牧兽医学报,2008,5:608～613.
7 冯焱,杨小军,姚军虎. 免疫应激对营养需要量及代谢调控机制的影响[J]. 中国畜牧兽医,2009,36(11):19～23.
8 刘玉兰,鲁晶,石君霞,等. 猪免疫应激研究进展[J]. 畜牧与兽医,2008,2:99～102.
9 江长优,张健,赵江贺. 牡蛎多糖增强小鼠免疫功能作用研究[J].中成药,2013,5:1062～1065.
10 李志. 牡蛎多糖的分离纯化及生物学活性研究[D].福州:福建农林大学,2009.
11 李钺. PPARγ对炎症状态下C57小鼠腹腔巨噬细胞胆固醇外流的影响[D].重庆:重庆医科大学,2007.
12 李萌,王磊,张立,等. 牡蛎多糖对小鼠腹腔巨噬细胞免疫功能的影响[J]. 中国药业,2013,17:15～16.
13 张艳军,李超柱,彭重威,等. 牡蛎多糖提取工艺的研究[J]. 食品研究与开发,2012,1:26～28.
14 陈永熙,王伟铭,周同,等. PPAR-γ作用及其相关信号转导途径[J]. 细胞生物学杂志,2006,3:382～386.
15 林先和. PPARγ参与免疫作用的研究进展[J]. 国外医学(免疫学分册),2004,5:291～293.
16 林沁. PPARγ和TZDs对肾小管间质炎症和纤维化的保护作用及其机制[D].上海:复旦大学,2003.
17 郝卫强,李京华,王俊德,等. 分子模型法在脂多糖研究中的应用[J]. 国外医学·生物医学工程分册,2003,5:203～207.
18 祝彬. PPAR-γ在哮喘小鼠气道的表达及临床意义[D].大连:大连医科大学,2012.
19 黄传贵,李勇,赵金华. 牡蛎多糖的分离和纯化[J]. 华西药学杂志,2009,4:439.
20 章慧娣,王伟铭,陈楠. PPARγ与肾脏炎症调节研究进展[J]. 中国中西医结合肾病杂志,2007,5:299～301.
21 隋昀原,沈国顺. 黄芪多糖对猪免疫功能影响的研究进展[J]. 中国畜牧兽医,2012,39(10):223～225.
22 Johnson R W. Inhibition of growth by pro-inflammatory cytokines:An integrated view[J]. Journal of Animal Science, 1997, 75:1244～1255.
23 Leininger M T, Portocarrero C P, Houseknecht K L. Peroxisome proliferator-activated receptor γ1 expression in porcine white blood cells:Dynamic regulation with acute endotoxemia[J]. Biochem Biophys Res Commun, 1999, 263(3):749～753.

[1]	陈慕天, 任妍, 张蕾, 李静, 谢思婕, 张艳, 张明, 梅秀丽. CsA对LPS诱导的子宫内膜基质细胞中IL-1β和IL-6表达的影响[J]. 中国畜牧兽医, 2020, 47(10): 3351-3360.
[2]	徐海旺, 余魁, 吴梦郡, 刘文凯, 李鹏, 易丹, 王蕾, 侯永清. 复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响[J]. 《中国畜牧兽医》, 2018, 45(8): 2197-2203.
[3]	张孝清, 童小明, 温伟业. 复方党参口服液对免疫应激蛋雏鸡免疫分子的影响[J]. 《中国畜牧兽医》, 2016, 43(8): 2066-2071.
[4]	韩慕俊;赵桂苹;赵景鹏;郑麦青;文杰. 维生素E水平和应激对雏鸡血浆皮质酮浓度的影响[J]. , 2009, 36(7): 60-62.
[5]	冯焱;杨小军;姚军虎. 免疫应激对营养需要量及代谢调控机制的影响[J]. , 2009, 36(11): 19-22.