CsA对LPS诱导的子宫内膜基质细胞中IL-1β和IL-6表达的影响

doi:10.16431/j.cnki.1671-7236.2020.10.035

摘要/Abstract

摘要： 本研究旨在探索免疫抑制剂环孢菌素A（CsA）对LPS诱导的子宫内膜基质细胞中促炎症细胞因子白细胞介素1β（IL-1β）和白细胞介素6（IL-6）表达的影响。母兔子宫内膜基质细胞经过体外培养和鉴定后，用1 μg/mL脂多糖（lipopolysaccharide，LPS）诱导构建子宫内膜基质细胞炎症模型，0 μg/mL LPS处理作对照组。用0、50、100、200和500 ng/mL CsA处理后，分别检测IL-1β和IL-6的表达水平。结果显示，与对照组相比，添加LPS显著诱导IL-1β和IL-6在子宫内膜基质细胞中的表达（P<0.05），CsA对子宫内膜基质细胞IL-1β和IL-6的表达无影响，但50、100、200和500 ng/mL CsA均极显著地抑制1 μg/mL LPS诱导的IL-1β和IL-6表达的上调（P<0.01）。因此，在子宫内膜基质细胞中，一定剂量的CsA能够有效抑制LPS诱导的子宫内膜基质细胞的免疫应激。本研究为雌性生殖道遭受革兰氏阴性菌感染引起免疫应激后用CsA治疗的可行性提供了依据，但在临床上的应用及其机制尚需进行深入研究。

关键词: 环孢菌素A; 脂多糖; 白细胞介素1β; 白细胞介素6; 免疫应激

Abstract: The aim of this study was to investigate the effect of immunosuppressant cyclosporine A (CsA) on the expression of proinflammatory cytokines interleukin 1 beta (IL-1β) and interleukin 6 (IL-6) in LPS-induced uterine endometrial stromas cells (ESCs).After cultured and identified in vitro,the inflammation model of the ESCs of female rabbits were successfully constructed by 1 μg/mL lipopolysaccharide (LPS),and 0 μg/mL LPS was used as the control.Then the expression levels of IL-1β and IL-6 were detected after treatment with 0,50,100,200 and 500 ng/mL CsA.The results showed that LPS significantly induced the expression of IL-1β and IL-6 in ESCs (P<0.05).Although CsA had no effect on the expression of IL-1β and IL-6,but 50,100,200 and 500 ng/mL CsA extremely significantly inhibited the upregulation of IL-1β and IL-6 induced by 1 μg/mL LPS (P<0.01).It suggested that a certain dose of CsA could effectively inhibit the LPS-induced immune stress in ESCs.This study provided a basis for the feasibility of CsA treatment after the genital tract of female infected by gram-negative bacteria,but its clinical application and mechanism still need to be further studied.

Key words: cyclosporine A; lipopolysaccharide; interleukin 1 beta; interleukin 6; immune stress

中图分类号:

S829.1

陈慕天, 任妍, 张蕾, 李静, 谢思婕, 张艳, 张明, 梅秀丽. CsA对LPS诱导的子宫内膜基质细胞中IL-1β和IL-6表达的影响[J]. 中国畜牧兽医, 2020, 47(10): 3351-3360.

CHEN Mutian, REN Yan, ZHANG Lei, LI Jing, XIE Sijie, ZHANG Yan, ZHANG Ming, MEI Xiuli. Effect of CsA on the Expression of IL-1β and IL-6 in Endometrial Stomas Cells Induced by LPS[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3351-3360.

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