猪嵴病毒非结构蛋白2C的生物信息学分析

›› 2014, Vol. 41 ›› Issue (11): 50-54.

猪嵴病毒非结构蛋白2C的生物信息学分析

陈秋勇¹, 胡崇伟¹, 陈如敬², 陈晓珞³, 林群群¹, 陈鹏强¹, 修金生¹

1. 福建农林大学动物科学学院, 福建福州 350002;
2. 福建省农业科学院畜牧兽医研究所, 福建省畜禽疫病防治工程技术研究中心, 福建福州 350013;
3. 龙岩市康顺养殖有限公司, 福建龙岩 364000

收稿日期:2014-03-06 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 修金生
作者简介:陈秋勇(1990-),男,福建人,硕士生,研究方向:猪病防控与研究。
基金资助:
福建省农业科学技术项目(2011-02)。

Bioinformatics Analysis of Nonstructural Protein 2C of Porcine Kobuvirus

CHEN Qiu-yong¹, HU Chong-wei¹, CHEN Ru-jing², CHEN Xiao-luo³, LIN Qun-qun¹, CHEN Peng-qiang¹, XIU Jin-sheng¹

1. College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China;
2. Fujian Animal Disease Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
3. Kang Shun Culture Limited Company, Longyan 364000, China

Received:2014-03-06 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 本研究运用RT-PCR方法从猪嵴病毒感染阳性样品中扩增出其非结构蛋白2C全基因,并将其克隆到T载体后进行序列测定。研究结果表明,猪嵴病毒非结构蛋白2C全长为1005 bp,编码335个氨基酸。所编的非结构蛋白2C大小为36.9297 ku,理论等电点为7.18;不稳定系数为39.41,属于稳定蛋白质类;脂肪族氨基酸指数为87.10,平均亲水性为-0.242。与其核苷酸同源性最高的是CH/HZ/2011株(GenBank登录号:JX827598),同源性为91.6%;同源性最低的是XX株(GenBank登录号:KC204684),同源性为88.8%。遗传进化分析结果显示,猪嵴病毒非结构蛋白2C在遗传进化树呈3个明显的结构分支,猪源嵴病毒匈牙利株和泰国株处于第Ⅲ遗传进化分支,猪嵴病毒中国分离株分处于第Ⅰ和第Ⅱ遗传进化分支,呈一定的区域流行性。

关键词: 猪嵴病毒; 非结构蛋白2C; 生物信息学分析

Abstract: To enrich the molecular epidemic information for the newly identified porcine kubovirus, the nonstructural protein 2C of porcine kobuvirus was amplified by reverse transcription-polymerase chain reaction method from the tissues of the piglets association with diarrhea in Fujian province, and then cloned and sequenced, bioinformatics analysis were conducted to investigate the structure and function of porcine kubovirus nonstructural protein 2C. The results demonstrated that porcine kobuvirus nonstructural protein 2C was 1005 bp in length, coding an open reading frame (ORF) with 335 amino acids. The molecular weight, theoretical isoelectric point, instability index (Ⅱ), aliphatic index and the Grand average of hydropathicity (GRAVY) of porcine kubovirus nonstructural protein 2C were 36.9297 ku, 7.18, 39.41, 87.10 and -0.242, respectively. The sequenced gene shared the highest homology with CH/HZ/2011 strain (GenBank accession number was JX827598), with 91.6%, while, the lowest homology with XX strain (GenBank accession number was KC204684), with 88.8%. The porcine kubovirus nonstructural protein 2C shared three different phylogenetic branches, porcine kubovirus Hungary isolates and Thailand isolates were at the branch Ⅲ, and the porcine kubovirus Chinese isolates distributed in the other two phylogenetic branches.

Key words: porcine kobuvirus; nonstructural protein 2C; bioinformatics analysis

中图分类号:

陈秋勇, 胡崇伟, 陈如敬, 陈晓珞, 林群群, 陈鹏强, 修金生. 猪嵴病毒非结构蛋白2C的生物信息学分析[J]. , 2014, 41(11): 50-54.

CHEN Qiu-yong, HU Chong-wei, CHEN Ru-jing, CHEN Xiao-luo, LIN Qun-qun, CHEN Peng-qiang, XIU Jin-sheng. Bioinformatics Analysis of Nonstructural Protein 2C of Porcine Kobuvirus[J]. , 2014, 41(11): 50-54.

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