贵州地方山羊促卵泡素受体基因部分外显子多态性研究

›› 2014, Vol. 41 ›› Issue (11): 198-204.

贵州地方山羊促卵泡素受体基因部分外显子多态性研究

龙威海, 丁玫, 冯文武, 许厚强, 陈伟, 张勇, 陈祥

贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵州贵阳 550025

收稿日期:2014-04-11 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 陈祥 E-mail:as.xchen@gzu.edu.cn
作者简介:龙威海(1988-),男,山东人,硕士生,研究方向:动物繁殖与动物生物技术。
基金资助:
贵州省高层次人才科研条件特助经费项目(TZJF-2011年-35号);贵州省科技重大专项[黔科合重大专项字(2013)6008];中央高校基本科研业务费专项资金项目(2010)。

Study on the Polymorphism for Part of FSHR Gene Exon in Guizhou Native Goat

LONG Wei-hai, DING Mei, FENG Wen-wu, XU Hou-qiang, CHEN Wei, ZHANG Yong, CHEN Xiang

Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Sciences, Guizhou University, Guiyang 550025, China

Received:2014-04-11 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 本试验以贵州白山羊、黔北麻羊和贵州黑山羊3个地方品种为研究对象,采用DNA池结合PCR直接测序法对促卵泡素受体(follicle stimulating hormone receptor, FSHR)基因进行单核苷酸多态性检测,同时结合在线软件预测不同基因型的mRNA的二级结构。结果表明,在3个山羊品种中共检测到3个SNPs位点:C126T、C1246A和A1412C,其中C126T和C1246A分别位于外显子1和外显子10中,且均为同义突变,A1412C位于内含子中。对外显子1和10中的2个SNPs位点进行生物信息学分析,结果显示均导致了mRNA二级结构发生改变。

关键词: 促卵泡素受体; SNPs; 外显子; 山羊

Abstract: In this study, Guizhou White goat, Qianbei Ma goat, Guizhou Black goat from Guizhou native goat breeds were selected for testing subjects, the technology of DNA pools and the direct sequencing of PCR products were used to detect the single nucleotide polymorphism (SNP) of follicle stimulating hormone receptor (FSHR) gene in goat. Moreover, through bioinformatics software to analyze the impact of secondary structure of RNA. The results showed that three SNPs (C126T,C1246A and A1412C) were found, among these SNPs, C126T and C1246A lay in the exon 1 and exon 10, which were sense mutation and the A1412C lay in intron. Bioinformatics analysis that the two SNPs in the exon 1 and exon 10 lead to the alteration of secondary structure of mRNA.

Key words: follicle stimulating hormone receptor; SNPs; exon; goat

中图分类号:

S813.3

龙威海, 丁玫, 冯文武, 许厚强, 陈伟, 张勇, 陈祥. 贵州地方山羊促卵泡素受体基因部分外显子多态性研究[J]. , 2014, 41(11): 198-204.

LONG Wei-hai, DING Mei, FENG Wen-wu, XU Hou-qiang, CHEN Wei, ZHANG Yong, CHEN Xiang. Study on the Polymorphism for Part of FSHR Gene Exon in Guizhou Native Goat[J]. , 2014, 41(11): 198-204.

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