›› 2013, Vol. 40 ›› Issue (2): 183-185.

• 疾病防治 • 上一篇    下一篇

猪链球菌7型的分离鉴定及药敏试验

戴金玲1,2, 许云龙3, 周玉龙3, 刘芳萍1, 李辰龙2, 宋新刚2   

  1. 1. 东北农业大学动物医学学院,东北哈尔滨 150030;
    2. 哈药集团生物疫苗有限公司,东北哈尔滨 150069;
    3. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
  • 收稿日期:2012-06-01 出版日期:2013-02-20 发布日期:2013-02-06
  • 通讯作者: 刘芳萍。E-mail:fangpingliu@126.com E-mail:fangpingliu@126.com
  • 作者简介:戴金玲(1976-),女,黑龙江人,硕士,主要从事疫苗研发工作。
  • 基金资助:
    黑龙江省科技厅重点推广项目(TC10B0701)。

Isolation, Identification and Drug Sensitivity Test of Streptococcus suis Serotype 7

DAI Jin-ling1,2, XU Yun-long3, ZHOU Yu-long3, LIU Fang-ping1, LI Chen-long2, SONG Xin-gang2   

  1. 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
    2. The First Bioproduct Manufactory of Heilongjiang Province, Harbin Pharmaceutical Group, Harbin 150069, China;
    3. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agriculture University, Daqing 163319, China
  • Received:2012-06-01 Online:2013-02-20 Published:2013-02-06

摘要: 某猪群暴发类似猪链球菌病,为确诊和防制该病,通过将病料接种到血液营养琼脂培养基和TSA培养基分离细菌,对分离菌株进行生化鉴定和PCR鉴定,并接种小白鼠进行动物试验和药敏试验。结果表明,分离的细菌为猪链球菌7型,对小白鼠具有致病性,且对恩诺沙星和头孢唑啉高度敏感。该结果对临床防制猪链球菌病具有参考意义。

关键词: 猪链球菌7型; 分离; 鉴定

Abstract: The purpose of this study was to diagnose, prevention and control the swine infectious diseases in certain pig farm. Disease samples were inoculated on blood agar medium and TSA culture medium for isolating pathogenic bacteria. The isolated strains were identified by biochemistry assay and PCR, and then animal experiment and drug sensitihty test was done. The results showed that the strains were identified as Streptococcus suis serotype 7 with pathogenicity to Mus. Drug sensitivity test showed that they were hypersensitive to enrofloxacin and cefazolin. This result was important for clinic to prevent and treat swine streptococcicosis.

Key words: Streptococcus suis serotypes 7; isolation; identification

中图分类号: