叉头框转录因子O亚族1基因对猪肾周脂肪沉积代谢的影响

›› 2013, Vol. 40 ›› Issue (2): 117-120.

叉头框转录因子O亚族1基因对猪肾周脂肪沉积代谢的影响

张野, 崔世泉, 狄生伟, 黄宣凯, 王希彪

东北农业大学动物科学技术学院,黑龙江哈尔滨 150030

收稿日期:2012-10-31 出版日期:2013-02-20 发布日期:2013-02-06
通讯作者: 王希彪(1965-),男,黑龙江人,教授,博士生导师,从事猪的育种与生产研究。E-mail:wxibiao1967@yahoo.com.cn E-mail:wxibiao1967@yahoo.com.cn
作者简介:张野(1987-),男,黑龙江人,硕士生,研究方向:动物遗传育种。
基金资助:
国家科技支撑计划项目(2008BADB2B02-5)。

Effect of Forkhead Box Transcription Factor O1 Gene on the Perirenal Fat Metabolism of the Pigs

ZHANG Ye, CUI Shi-quan, DI Sheng-wei, HUANG Xuan-kai, WANG Xi-biao

Northeast Agricultural University, College of Animal Science and Technology, Harbin 150030, China

Received:2012-10-31 Online:2013-02-20 Published:2013-02-06

摘要/Abstract

摘要： 叉头框转录因子O亚族1(forkhead box transcription factor O1,FoxO1)在细胞增殖、分化过程中发挥着重要的作用。采用实时荧光定量PCR技术,对不同月龄民猪和长白猪肾周脂肪中FoxO1、过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptorγ,PPARγ)和CAAT区/增强子结合蛋白α(CAAT/enhancer binding protein α,C/EBPα)基因mRNA表达量进行检测,探讨FoxO1在猪肾周脂肪代谢过程中的作用。结果显示,2品种猪肾周脂肪2.5月龄FoxO1基因mRNA表达量极显著高于1、6、8月龄(P<0.01);分析FoxO1与PPARγ、C/EBPα基因的表达关系,结果发现在2.5月龄后,FoxO1 mRNA的表达与PPARγ、C/EBPα相反,说明FoxO1可能通过抑制这2个基因的表达来减少脂肪沉积。

关键词: FoxO1; PPARγ; C/EBPα; 实时荧光定量PCR; 肾周脂肪

Abstract: Forkhead box transcription factor O1(FoxO1) played a critical role in cell proliferation and differentiation of mammals. An experiment was conducted to determine the mRNA expression of FoxO1,PPAR γ and C/EBPα from perirenal fat of pigs by using Real-time PCR to study the role of FoxO1 in fat metabolism. The results indicated that in perirenal adipose,the expression of FoxO1 mRNA in Min pigs and Landrace of 2.5 months of age was significant higher than that in pigs of 1,6 and 8 months of age (P<0.01). According to the analysis of the relationship between mRNA expression of FoxO1 and PPARγ,C/EBPα gene,the expression trend of FoxO1 was contrary to PPARγ,C/EBPα after 2 months,which manifested that FoxO1 inhibited fat deposition through inhibiting PPARγ,C/EBPα mRNA expression. The results suggested that FoxO1 played an inhibitting role in fat deposition of pigs.

Key words: forkhead box transcription factor O1(FoxO1); PPARγ; C/EBPα; Real-time PCR; perirenal fat

中图分类号:

Q786

张野, 崔世泉, 狄生伟, 黄宣凯, 王希彪. 叉头框转录因子O亚族1基因对猪肾周脂肪沉积代谢的影响[J]. , 2013, 40(2): 117-120.

ZHANG Ye, CUI Shi-quan, DI Sheng-wei, HUANG Xuan-kai, WANG Xi-biao. Effect of Forkhead Box Transcription Factor O1 Gene on the Perirenal Fat Metabolism of the Pigs[J]. , 2013, 40(2): 117-120.

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