安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析

›› 2013, Vol. 40 ›› Issue (11): 195-200.

安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析

戴银¹, 赵瑞宏¹, 胡晓苗¹, 张丹俊¹, 夏松林², 盛昌树³, 杨文超³, 钟国发⁴

1. 安徽省农科院畜牧兽医研究所, 安徽合肥 230031;
2. 芜湖市动物疫病预防与控制中心, 安徽芜湖 241000;
3. 繁昌县动物疫病预防与控制中心, 安徽繁昌 241200;
4. 繁昌县五华鸡保种场, 安徽繁昌 241209

收稿日期:2013-04-26 出版日期:2013-11-20 发布日期:2013-12-19
通讯作者: 张丹俊
作者简介:戴银(1980-),女,安徽人,博士,助理研究员,研究方向:兽医微生物与免疫学。
基金资助:
国家蛋鸡产业技术体系项目(CARS-41);国家自然科学基金项目(31302044);安徽省科技攻关项目(11010302119);院科技创新团队(11C0404);院长青年创新基金项目(11B0418)。

Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province

DAI Yin¹, ZHAO Rui-hong¹, HU Xiao-miao¹, ZHANG Dan-jun¹, XIA Song-lin², SHENG Chang-shu³, YANG Wen-chao³, ZHONG Guo-fa⁴

1. Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
2. Wuhu Center for Animal Disease Control and Prevention, Wuhu 241000, China;
3. Center for Animal Disease Control and Prevention of Fanchang County, Fanchang 241200, China;
4. Wuhua Chicken Breed Protection Company of Fanchang County, Fanchang 241209, China

Received:2013-04-26 Online:2013-11-20 Published:2013-12-19

摘要/Abstract

摘要： 为进一步了解J亚群禽白血病病毒(avian leukosis virus subgroup J,ALV-J)gp85基因的结构、功能及其遗传变异趋势,采用PCR方法克隆了安徽省地方品种五华鸡ALV-J gp85基因序列ALV-J-WH,并将该序列与GenBank数据库中登录的15个gp85基因序列进行了比对分析。结果显示,ALV-J-WH与所比对的氨基酸序列同源性介于85.9%~96.2%之间,与其同源性最高的是来自于国内ALV-J毒株的JN378888基因序列,进化树也证实两者亲缘关系较近;此外,相对其他ALV-J gp85氨基酸序列,ALV-J-WH在223位氨基酸后有10个氨基酸的缺失;对所有全长序列分析结果表明,共有71个可变氨基酸位点,10个高度变异位点,尤其是hr1和hr2区域分布较多。结果表明,ALV-J-WH与国内部分ALV-J毒株分离gp85基因来自共同的原始病毒,但其具有独特的序列特征;ALV-J gp85基因高度易变,部分高频率突变的氨基酸位点可能是ALV-J在抗体免疫选择压下的作用位点。

关键词: J亚群禽白血病病毒; gp85基因; 序列特征

Abstract: In order to further understand the structure,function and genetic variation of ALV-J gp85 gene,the gene was cloned by PCR in Anhui province,which named as ALV-J-WH. We compared ALV-J-WH with fifteen amino acid sequence of ALV-J gp85 genes in GenBank database. The results showed that the homology between ALV-J-WH and the other sequences ranged from 85.9% to 96.2%, the highest homology with ALV-J-WH were JN378888 sequences isolated from China. Evolution analysis further revealed that they had a close genetic relationship. Moreover,the deletion of a 10 amino acid was observed in the ALV-J-WH between position 223 and 224 of amino acid sequence. Alignment of the full-length sequences revealed 71 variable amino acid sites,10 sites had high mutation frequency,expecially sites in hr1 and hr2 domains. The results confirmed that ALV-J-WH and partial genes of strains isolated from China evolved from common strains,but the sequence was unique. ALV-J gp85 gene was highly variable,and some amino acids with high degree of mutation frequency might be action sites of ALV-J antibody under immune selective pressure.

Key words: avian leukosis virus subgroup J; gp85 gene; sequence feature

中图分类号:

S831

戴银, 赵瑞宏, 胡晓苗, 张丹俊, 夏松林, 盛昌树, 杨文超, 钟国发. 安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析[J]. , 2013, 40(11): 195-200.

DAI Yin, ZHAO Rui-hong, HU Xiao-miao, ZHANG Dan-jun, XIA Song-lin, SHENG Chang-shu, YANG Wen-chao, ZHONG Guo-fa. Sequence Feature Analysis of gp85 Gene of Avian Leukosis Virus Subgroup J in Wuhua Chickens in Anhui Province[J]. , 2013, 40(11): 195-200.

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