奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定

›› 2013, Vol. ›› Issue (6): 246-249.

• 经验交流 • 上一篇

奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定

邓慧媛^1,2, 李志忠², 张凯¹, 李建喜¹

1. 中国农业科学院兰州畜牧与兽药研究所, 甘肃兰州 730050;
2. 兰州理工大学生命与工程学院, 甘肃兰州 730050

收稿日期:2012-10-30 出版日期:2013-06-20 发布日期:2013-06-20
通讯作者: 李建喜 E-mail:lzjianxil@163.com
作者简介:邓慧媛(1987-),女,甘肃人,硕士,研究方向:生物化学与分子生物学。
基金资助:
国家自然科学基金(31072162);现代农业奶牛产业技术体系(CARS-37-06)。

Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid

DENG Hui-yuan^1,2, LI Zhi-zhong², ZHANG Kai¹, LI Jian-xi¹

1. Institute of Lanzhou Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China

Received:2012-10-30 Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要： 采用平板划线分离法从健康奶牛瘤胃液中分离得到1株细菌Y-1,体外模拟瘤胃环境增殖Y-1,进行形态学观察、生化反应和16S rDNA基因同源性比较,并对其进行系统发育树的构建等系统鉴定。结果表明,该细菌为浸麻类芽孢杆菌(Paenibacillus macerans),本研究还对瘤胃液中存在该菌的原因做了分析。

关键词: 浸麻类芽孢杆菌; 形态学鉴定; 生化管鉴定; 16S rDNA测序

Abstract: We used flat crossed separation method to get a bacterial Y-1, which was isolated from a cow rumen fluid. Simulating rumen environment in vitro to proliferate Y-1. The test adopted the methods, which include morphological observation, biochemical reactions and 16S rDNA gene homology comparison, and constructed the phylogenetic tree for Y-1.The results indicated that Y-1 was Paenibacillus macerans strain, and analyzed the reasons for the presence of bacteria in the rumen fluid.

Key words: Paenibacillus macerans; morphological identification; biochemical tube identification; 16S rDNA sequcing

中图分类号:

S823

邓慧媛, 李志忠, 张凯, 李建喜. 奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定[J]. , 2013, (6): 246-249.

DENG Hui-yuan, LI Zhi-zhong, ZHANG Kai, LI Jian-xi. Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid[J]. , 2013, (6): 246-249.

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奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定

Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid

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