蛋白质组学技术在病毒及其感染机制研究中的应用

›› 2013, Vol. ›› Issue (5): 75-79.

蛋白质组学技术在病毒及其感染机制研究中的应用

孙成友¹, 李娟², 刘雨田¹, 李玉保³, 迟田英¹, 宋芳¹, 于小静¹, 王志亮¹

1. 中国动物卫生与流行病学中心国家疯牛病参考实验室, 山东青岛 266032;
2. 山东农业大学动物医学院, 山东泰安 271018;
3. 聊城大学农学院, 山东聊城 252029

收稿日期:2012-09-29 出版日期:2013-05-20 发布日期:2013-05-27
通讯作者: 王志亮,研究方向:动物病毒病和动物传染病。Tel:0532-87839188;E-mail:zlwang111@yahoo.com.cn E-mail:zlwang111@yahoo.com.cn
作者简介:孙成友(1968-),男,黑龙江人,兽医师,学士,研究方向:国家疯牛病痒病检测防控。李娟(1988-),女,山东人,硕士生,研究方向:动物临床病理学。孙成友与李娟对本文具有同等贡献,并列为第一作者。
基金资助:
国家自然科学基金(31101787);山东省自然科学基金(ZR2010CM035)。

The Application of Proteomics Technologies in the Research of Virus and its Infection Mechanism

SUN Cheng-you¹, LI Juan², LIU Yu-tian¹, LI Yu-bao³, CHI Tian-ying¹, SONG Fang¹, YU Xiao-jing¹, WANG Zhi-liang¹

1. China Animal Health and Epidemiology Center, Qingdao 266032, China;
2. College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China;
3. College of Agriculture, Liaocheng University, Liaocheng 252029, China

Received:2012-09-29 Online:2013-05-20 Published:2013-05-27

摘要/Abstract

摘要： 在病毒学领域中,如双向差异凝胶电泳、偶联应用质谱和稳定同位素标记等的蛋白质组学技术正在被广泛应用,可从整体层面上分析组织或细胞内动态变化的蛋白质组成、表达水平与翻译后修饰,探索蛋白质的功能及蛋白质之间相互作用与联系,有助于病毒感染及病毒与宿主间的相互作用机制的研究,推动病毒相关疾病诊断和治疗的发展。作者简要论述了近年来蛋白质组学技术在病毒及其感染机制研究中的应用,并对其在病毒学研究中的应用前景作了展望。

关键词: 蛋白质组学; 病毒; 感染机制; 应用

Abstract: In the field of virology, the proteomics technologies wildly is used,such as two-dimensional difference electrophoresis, mass spectrometry, Stable-isotope labeling by amino acids in cell culture, and so on. These mainly methods are applied to analyse the dynamic changes of the components, expression level and post-translational modification of proteins from the whole level, then to explore the proteins function and the interact and correlate with them. These will contribute to research the mechanism of viruses infecting the hosts and the interactions, and promote development of diagnosis and treatment of the diseases caused by viruses. In this study, the applications of proteomics technologies in the research of virus and its infection mechanism were discussed and the prospect in virology study would also be forecasted.

Key words: proteomic; virus; infection mechanism; application

中图分类号:

Q816

孙成友, 李娟, 刘雨田, 李玉保, 迟田英, 宋芳, 于小静, 王志亮. 蛋白质组学技术在病毒及其感染机制研究中的应用[J]. , 2013, (5): 75-79.

SUN Cheng-you, LI Juan, LIU Yu-tian, LI Yu-bao, CHI Tian-ying, SONG Fang, YU Xiao-jing, WANG Zhi-liang. The Application of Proteomics Technologies in the Research of Virus and its Infection Mechanism[J]. China Animal Husbandry & Veterinary Medicine, 2013, (5): 75-79.

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