RNA干扰抑制家畜病毒感染的研究进展

›› 2013, Vol. ›› Issue (5): 61-66.

RNA干扰抑制家畜病毒感染的研究进展

黄双^1,2, 王凤雪², 温永俊²

1. 沈阳农业大学, 辽宁沈阳 110866;
2. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学国家重点实验室, 吉林长春 130122

收稿日期:2012-10-24 出版日期:2013-05-20 发布日期:2013-05-27
通讯作者: 温永俊(1979-),男,内蒙人,博士,副研究员,主要从事动物分子病毒和免疫学方面的研究。E-mail:yongjunwen@126.com;Tel:0431-81919840 E-mail:yongjunwen@126.com
作者简介:黄双(1987-),女,辽宁人,硕士,研究方向:动物病毒分子生物学。
基金资助:
国家863项目"新型重大动物疫病疫苗与诊断试剂创制及生产工艺创新"(2011AA10A213);吉林省特种经济动物疫病防控研究创新团队(20121823)。

Advancement on the Inhibition of Viral Replication by RNA Interference in Animals

HUANG Shuang^1,2, WANG Feng-xue², WEN Yong-jun²

1. Shenyang Agricultural University, Shenyang 110866, China;
2. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130122, China

Received:2012-10-24 Online:2013-05-20 Published:2013-05-27

摘要/Abstract

摘要： RNAi(RNA-mediated interference)是一种由序列特异性的dsRNA(double stranded RNA)作为触发器来触发同源mRNA降解从而引起基因沉默的细胞机制。利用RNAi方法可以通过沉默特异基因来对目的基因进行功能性分析,另外通过干扰RNA抑制病原性基因的表达来进行病毒性疾病治疗的研究已取得了显著的成就。作者对RNAi的作用机制、RNAi在抑制病毒复制和抵抗病毒感染动物方面的研究进展及应用前景和存在问题进行了综述。

关键词: RNA干扰; 病毒感染; 复制抑制

Abstract: RNA-mediated interference (RNAi) is a conserved gene-silencing mechanism, where by the doublestranded RNA matching is used as a signal to trigger the sequence-specific degradation of homologous mRNA. RNAi has become a valuable tool for analysis of gene function through suppression of specific gene products. In addition, RNAi has shown great promise for use in antiviral strategies designed to suppress the expression of pathogenic genes. The present review elaborates the mechanism of RNA interference, the development and application about RNAi inhibiting the virus replication and infection in animals, and the issued obstacles.

Key words: RNA-mediated interference; viral infection; replication inhibition

中图分类号:

S852.7

黄双, 王凤雪, 温永俊. RNA干扰抑制家畜病毒感染的研究进展[J]. , 2013, (5): 61-66.

HUANG Shuang, WANG Feng-xue, WEN Yong-jun. Advancement on the Inhibition of Viral Replication by RNA Interference in Animals[J]. , 2013, (5): 61-66.

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