›› 2013, Vol. ›› Issue (5): 40-43.

• 生物技术 • 上一篇    下一篇

一株脱氨除臭菌的筛选鉴定及其脱氮性能研究

刘平1, 陈芳艳3, 谢永福1, 况绍祥1, 刘金1, 张克山1, 任柏桦1, 王林川1,2   

  1. 1. 华南农业大学兽医学院, 广东广州 510642;
    2. 广东大华农动物保健品股份有限公司, 广东新兴 527400;
    3. 华南农业大学动物科学学院, 广东广州 510642
  • 收稿日期:2012-10-30 出版日期:2013-05-20 发布日期:2013-05-27
  • 通讯作者: 王林川(1965-),男,教授,博士,博士生导师。E-mail:lcwang@scau.edu.cn E-mail:lcwang@scau.edu.cn
  • 作者简介:刘平(1986-),男,湖北人,硕士,研究方向:动物传染病。

Screening and Identification of a Denitrifying and Deodorizing Strain and Study on its Denitrification Ability

LIU Ping1, CHEN Fang-yan3, XIE Yong-fu1, KUANG Shao-xiang1, LIU Jin1, ZHANG Ke-shan1, REN Bai-hua1, WANG Lin-chuan1,2   

  1. 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
    2. Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China;
    3. College of Animal Science, South China Agricultural University, Guangzhou 510642, China
  • Received:2012-10-30 Online:2013-05-20 Published:2013-05-27

摘要: 用感官法初筛选出18株细菌,再通过定性复筛试验,最后从鸡粪中分离出1株具有明显脱氨除臭作用的细菌JNX-8;经菌落形态特征观察、生化试验、16S rDNA测序等过程,鉴定为蜡状芽孢杆菌(Bacillus cereus)。对该菌脱氮性能进行了研究,确定其对硝基氮和氨氮的去除率分别达到51.71%和66.28%。结果显示,菌株JNX-8在鸡粪发酵除臭中具有良好的应用前景。

关键词: 蜡状芽孢杆菌; 鉴定; 脱氮性能

Abstract: 18 bacteria strains were isolated through sensory screening test, then through qualitative rescreening test, JNX-8 strain was isolated from poultry litter because of the best denitrification and deodorization ability. Based on morphology and physiology of colony and 16S rDNA sequence analysis, the strain was characterized as Bacillus cereus. The denitrification ability of JNX-8 strain was studied, 51.71% of NO3--N and 66.28% of NH4+-N were respectively removed by JNX-8. Therefore, JNX-8 had good application prospects in the denitrification and deodorization of poultry litter.

Key words: Bacillus cereus; identification; denitrification ability

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