J亚型禽白血病病毒感染黄鸡病毒血症和抗体反应动态的研究

中国畜牧兽医

J亚型禽白血病病毒感染黄鸡病毒血症和抗体反应动态的研究

王小芬, 刘雪美, 廖亚琳, 吴晓婵, 廖明, 曹伟胜

华南农业大学兽医学院,农业部动物疫病防控重点开放实验室,广东广州 510642

收稿日期:2012-02-23 出版日期:2012-08-20 发布日期:2012-08-24
通讯作者: 曹伟胜 E-mail:caoweish@scau.edu.cn
作者简介:王小芬(1987-),女,河南人,硕士生,主要从事动物病毒学研究。
基金资助:
公益性行业(农业)科研专项(201203055);NSFC-广东联合基金(U0831002);国家肉鸡产业技术体系项目(CARS-24-G09);广东省自然科学基金项目(S2011010001946)。

Research of Dynamics of Viremia and Antibody Responses of Yellow Chicken Infected by Avian Leukosis Virus Subgroup J

WANG Xiao-fen, LIU Xue-mei, LIAO Ya-lin, WU Xiao-chan, LIAO Ming, CAO Wei-sheng

Key Laboratory of Animal Disease Control and Prevention,Ministry of Agriculture, College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China

Received:2012-02-23 Online:2012-08-20 Published:2012-08-24

摘要/Abstract

摘要： 将血管瘤病变型相关的J亚型禽白血病病毒(ALV-J)SCAU-HN06株通过尿囊腔途径接种黄鸡11日龄鸡胚,对其出壳后不同时间点(1、2、3、4、5、6、7、8、9、10、12、14、16、18、20周)的病毒血症、ALV-J特异性抗体及泄殖腔拭子p27抗原分别进行了动态检测。试验结果表明,SCAU-HN06组在1周即检测到病毒血症,7周时有一个高峰,9周后病毒血症阳性率始终维持在80%以上;从6周时开始检测到ALV-J特异性抗体,但监测过程中抗体阳性率最高仅为33.3%(18周),其余均在22%以下;泄殖腔p27抗原阳性率在前3周迅速升高,3周时达到顶峰,随后维持较高的阳性率。由研究结果可知,经鸡胚接种ALV-J的感染黄鸡可导致持续性病毒血症,并容易产生免疫耐受;这项研究可为黄鸡ALV-J的防控与净化提供科学依据。

关键词: J亚群禽白血病病毒; 黄鸡; 病毒血症; 动态

Abstract: The dynamics of viremia,ALV-J antibody response in yellow chickens and p27 antigen shedding in cloacal swabs were examined respectively after the 11-day-old yellow chicken embryos were inoculated with hemangioma related ALV-J SCAU-HN06 isolate via allantoic cavity. The results showed that viremia could be detected as early as 1 week post hatch,there was a viremia peak at 7 week,and the positive rate of viremia remained more than 80% continuously since 9 week. ALV-J antibody response could be detected as early as 6 week by ELISA,the positive rate of antibody was no more than 33.3% during the trial. The positive rate of p27 antigen in cloacal shedding rised quickly,reached peak at 3 week,then kept at high level. The study suggested that ALV-J isolate infection via egg inoculation could result in persistent viremia and immunotolerance. The study provided the basis for ALV-J control and eradication in yellow chickens.

Key words: subgroup J avian leukosis virus(ALV-J); yellow chicken; viremia; dynamic

中图分类号:

S858.31

王小芬, 刘雪美, 廖亚琳, 吴晓婵, 廖明, 曹伟胜. J亚型禽白血病病毒感染黄鸡病毒血症和抗体反应动态的研究[J]. 中国畜牧兽医.

WANG Xiao-fen, LIU Xue-mei, LIAO Ya-lin, WU Xiao-chan, LIAO Ming, CAO Wei-sheng. Research of Dynamics of Viremia and Antibody Responses of Yellow Chicken Infected by Avian Leukosis Virus Subgroup J[J]. .

参考文献

1 冯少珍,李娇,吴晓婵,等.人工感染血管瘤病变型ALV-J对鸡免疫器官的影响[J].中国兽医科学,2011,41(8):784~788.

2 成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45(4):584~587.

3 成子强,赵振华,张利,等.禽白血病病毒J亚群(ALV-J)的攻毒试验[J].中国兽医学报,2004,24(3):219~222.

4 孙贝贝,崔治中,张青婵,等.ALV-J人工感染鸡病毒血症和抗体反应动态[J].中国农业科学,2009,42(11):4069~4076.

5 杜岩,崔治中,秦爱建,等.从市场商品肉鸡中检出J亚群禽白血病病毒[J].中国家禽学报,1999,1:1~4.

6 张丹俊,戴银,赵瑞宏,等.禽白血病病毒J亚群感染对五华鸡生产性能和免疫性能的影响[J].中国畜牧兽医,2012,39(2):141~143.

7 张洪海,刘青,邱波,等.地方柴鸡中J亚群禽白血病与马立克氏病的混合感染[J].畜牧兽医学报,2009,40(8):1215~1221.

8 李艳,崔治中,孙淑红.黄羽肉鸡J亚群白血病病毒的分子生物学特性和致病性[J].病毒学报,2007,23(3):207~211.

9 辛朝安,曹伟胜,罗开健,等.鸡血管瘤型白血病诊断初报[J].养禽与禽病防治,2006,11:2.

10赖汉漳.血管瘤型禽白血病病原学研究及ALV感染性克隆构建.广州:华南农业大学,2009.

11Saif Y M.禽病学[M].第11版. 苏敬良,等译.北京:中国农业出版社,2005.

12Himly M,Foster D N,Bottoli I,et al. The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses[J].Virology,1998,248:295~304.

13Lai H Z,Zhang H N,Ning Z Y,et al. Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens[J].Veterinary Microbiology,2011,151:275~283.

14Pandiri A R,Gimeno I M,Reed W M,et al. Subgroup J avian leukosis virus-induced histiocytic sarcomatosis occurs only in persistently viremic but not immunotolerized meat-type chickens[J].Vet Pathol,2009,46:282~287.

15Pandiri A R,Mays J K,Silva R F,et al. Subgroup J avian leukosis virus neutralizing antibody escape variants contribute to viral persistence in meat-type chickens[J].Avian Dis,2010,54(2):848~856.

16Pandiri A R,Reed W M,Mays J K,et al. Influence of strain,dose of virus,and age at inoculation on subgroup J avian leukosis virus persistence,antibody response,and oncogenicity in commercial meat-type chickens[J].Avian Dis,2007,51(3):725~732.

17Payne L N,Brown S R,Bumstead N,et al. A novel subgroup of exogenous avian leukosis virus in chickens[J].Journal of General Virology,1991,72:801~807.

18Russell P H,Ahmad K,Howes K,et al. Some chickens which are viraemic with subgroup J avian leukosis virus have antibody-forming cells but no circulating antibody[J].Research in Veterinary Science,1997,63:81~83.

19Witter R L,Bacon L D,Hunt H D,et al. Avian leukosis virus subgroup J infection profiles in broiler breeder chickens:association with virus transmission to progeny[J].Avian Dis,2000,44(4):913~931.

20Zhang H N,Lai H Z,Qi Y,et al. An ALV-J isolate is responsible for spontaneous haemangiomas in layer chickens in China[J].Avian Pathology,2011,40(3):261~267.

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