一株鸡传染性法氏囊病病毒的分离鉴定

›› 2012, Vol. 39 ›› Issue (6): 207-211.

一株鸡传染性法氏囊病病毒的分离鉴定

赵云英¹, 张燕欣¹, 张文生², 李富桂¹

1. 天津农学院动物科学系,天津 300384;
2. 天津职业大学生物与环境工程学院,天津 300402

修回日期:2011-12-16 出版日期:2012-06-20 发布日期:2012-07-02
通讯作者: 李富桂(1960－),女,天津人,教授,博士,主要从事兽医病理学、畜禽疾病诊断、综合防治技术的教学、科研工作。E-mail:lifugui@eyou.com E-mail:lifugui@eyou.com
作者简介:赵云英(1986－),女,河北人,硕士生,主要从事预防兽医学方面的研究。

Isolation and Identification of One Strain Infectious Bursal Disease Virus

ZHAO Yun-ying¹, ZHANG Yan-xin¹, ZHANG Wen-sheng², LI Fu-gui¹

1. Department of Animal Science,Tianjin Agricultural University, Tianjin 300384, China;
2. College of Biology and Environmental Engineering, Tianjin Vocational Institute, Tianjin 300402, China

Revised:2011-12-16 Online:2012-06-20 Published:2012-07-02

摘要/Abstract

摘要： 从天津地区免疫失败的鸡群中分离一株IBDV(命名TJ-hg),应用血清学AGP试验结果可见明显的白色沉淀线;应用逆转录酶—聚合酶链式反应(RT-PCR)扩增的 IBDV VP2 基因高变区(vVP2)及其序列分析的结果表明,TJ-hg株与超强毒株的核苷酸同源性均在90%以上,氨基酸同源性达98.8%~99.3%,且高变区中特征性氨基酸的变化与超强毒株完全一致,TJ-hg株在第一亲水区氨基酸Q219P发生突变。研究结果表明,TJ-hg株属于超强毒株,但又与标准毒株存在差异。

关键词: IBDV; 超强毒株; 分子鉴定

Abstract: One isolate of infectious bursal disease virus (IBDV),was isolated from the vaccination failure chicken. Through serologic AGP experiment,results showed obvious white precipitation line; the high variable (hv) region of VP2 genes were amplified by RT-PCR (reverse transcriptase polymerase chain reaction) and were compared for their sequences. It was indicated that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90% and 98.8% to 99.3% at nucleotide level,respectively. And the change of amino acid was complement in high variable (hv) region of VP2 genes. The mutations amino acid Q219P located in the first hydrophilic region of TJ-hg strain. The research demonstrated that TJ-hg strain belonged to vvIBDV,but it was different from standard strain.

Key words: IBDV; very virulent strain; molecular identification

中图分类号:

S852.65

赵云英, 张燕欣, 张文生, 李富桂. 一株鸡传染性法氏囊病病毒的分离鉴定[J]. , 2012, 39(6): 207-211.

ZHAO Yun-ying, ZHANG Yan-xin, ZHANG Wen-sheng, LI Fu-gui. Isolation and Identification of One Strain Infectious Bursal Disease Virus[J]. , 2012, 39(6): 207-211.

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