青年母猪不同生殖器官神经营养因子-4/5的表达研究

›› 2012, Vol. 39 ›› Issue (3): 41-46.

青年母猪不同生殖器官神经营养因子-4/5的表达研究

张伟¹, 易康乐², 周虚¹

1. 吉林大学畜牧兽医学院,吉林长春 130062;2. 湖南省畜牧兽医研究所,湖南长沙 410000

收稿日期:2011-08-01 修回日期:1900-01-01 出版日期:2012-03-20 发布日期:2012-03-20
通讯作者: 周虚

Expression of Neurotrophin-4/5 in the Reproductive Organs of the Prepubertal Gilts

ZHANG Wei¹, YI Kang-le², ZHOU Xu¹

1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2. Hunan Institute of Animal and Veterinary Science, Changsha 410000, China

Received:2011-08-01 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20

摘要/Abstract

摘要： 本研究旨在探讨神经营养因子-4/5(neuroteophin-4/5, NT-4/5)在青年母猪卵泡期与黄体期卵巢、输卵管及子宫3种生殖器官中表达情况。采用实时荧光定量RT-PCR方法与Western blotting技术对青年母猪卵泡期与黄体期卵巢、输卵管及子宫中的NT-4/5 mRNA与蛋白质表达进行检测。结果表明,NT-4/5 mRNA与蛋白质在卵泡期与黄体期卵巢、输卵管及子宫中均有表达,且卵泡期卵巢、子宫NT-4/5 mRNA表达水平均显著高于黄体期(P＜0.05),而卵泡期输卵管NT-4/5 mRNA表达水平显著低于黄体期(P＜0.05)。试验结果为进一步研究NT-4/5参与青年母猪生殖道生理功能的调节奠定基础。

关键词: 生殖器官; NT-4/5; 青年母猪

Abstract: The expression of neurotrophin-4/5 (NT-4/5) mRNA and protein in ovary, oviduct and uterus during the follicle and luteal phase of the pubertal gilts were investigated by quantitative real-time PCR and Western blotting. The results showed that the mRNA and protein of NT-4/5 were present in ovary, oviduct and uterus during the follicle and luteal phase. In addition, the expression amount of NT-4/5 mRNA in ovary, uterus during the follicle stage were significantly higher than the luteal phase (P＜0.05), however, the expression level of NT-4/5 mRNA in oviduct during the follicle phase was significantly lower than the luteal phase(P＜0.05). In collection, these results strongly suggest that local production of the NT-4/5 may particulate in the regulation of reproductive functions in the reproductive functions in the reproductive tract of the prepubertal gilts.

Key words: reproductive organs; NT-4/5; prepubertal gilt

中图分类号:

Q421

张伟;易康乐;周虚. 青年母猪不同生殖器官神经营养因子-4/5的表达研究[J]. , 2012, 39(3): 41-46.

ZHANG Wei;YI Kang-le;ZHOU Xu. Expression of Neurotrophin-4/5 in the Reproductive Organs of the Prepubertal Gilts[J]. , 2012, 39(3): 41-46.

[1]	王志亮, 杨喜喜, 铁雅楠, 伏晓玉, 王欣荣. 超排处理对家兔生殖器官形态及激素水平的影响[J]. 中国畜牧兽医, 2022, 49(6): 2250-2258.
[2]	刘明超, 李作臣, 王晓明, 尹熙俊, 崔成都. 雌性五指山猪生殖器官生长发育的研究[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 650-657.
[3]	李成娇;朱晓玲;李万宏;刘卓;石德顺;刘庆友;周虚. 促性腺激素受体在雌性水牛生殖器官的表达定位研究[J]. , 2011, 38(4): 66-69.
[4]	姜怀志;杨雨江 . 辽宁绒山羊生殖器官形态学参数的研究[J]. , 2007, 34(11): 142-143.
[5]	关现军. 鸡生殖器官的病理剖检变化与产蛋异常[J]. , 2007, 34(10): 149-150.
[6]	关现军. 鸡生殖器官的病理剖检变化与产蛋异常[J]. , 2007, 34(10): 117-118.