猪β干扰素的基因改造及真核表达

›› 2011, Vol. 38 ›› Issue (9): 125-129.

猪β干扰素的基因改造及真核表达

唐明森¹, 陈瑞爱³, 刘健², 裴党帅¹, 郑念广¹, 罗满林^1,3

1. 华南农业大学兽医学院,广东广州 510642;2. 山东齐鲁动物保健品股份有限公司,山东济南 250100;3. 大华农动物保健品股份有限公司,广东新兴 527400

收稿日期:2011-11-22 修回日期:1900-01-01 出版日期:2011-09-20 发布日期:2011-09-20
通讯作者: 罗满林

Genes Modify and Expression of Porcine Interferon-beta in Yeast Pichia pasteris

TANG Ming-sen¹, CHEN Rui-ai³, LIU Jian², PEI Dang-shuai¹, ZHENG Nian-guang¹, LUO Man-lin^1,3

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510462, China;2. Qilu Animal Health Products Co., Ltd., Jinan 250100, China;3. Guangdong Dahuanong Animal Health Products Co., Ltd., Xinxing 527400, China

Received:2011-11-22 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

摘要/Abstract

摘要： 本试验选择高表达密码子重新设计合成猪β干扰素成熟肽核苷酸,通过设计特异的引物,在毕赤酵母表达系统中表达PoIFN-β,获得分泌表达的高活性重组PoIFN-β,并采用非洲绿猴肾细胞(Vero)-水泡性口炎病毒(VSV)系统以细胞病变抑制法测定重组猪β干扰素的效价,为指导重组PoIFN-β的临床使用和研究其生物学功能提供基础。

关键词: 猪β干扰素; 酵母表达; 高活性

Abstract: The mature nucleotide peptide of porcine interferon-β was redesigned and synthesized by high expression codon in the test,designed specific primers,porcine interferon-β was expressed through Pichia pastoris expression system,recombinant PoIFN-β was measured by cytopathic effects with Vero - VSV system, the clinical application and biological function of recombinant PoIFN-β were provided by the research.

Key words: porcine interferon-β; expression in Pichia pastoris; high activity

中图分类号:

唐明森;陈瑞爱;刘健;裴党帅;郑念广;罗满林;. 猪β干扰素的基因改造及真核表达[J]. , 2011, 38(9): 125-129.

TANG Ming-sen;CHEN Rui-ai;LIU Jian;PEI Dang-shuai;ZHENG Nian-guang;LUO Man-lin;. Genes Modify and Expression of Porcine Interferon-beta in Yeast Pichia pasteris[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(9): 125-129.

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