辽西地区鸡大肠杆菌分离鉴定、药敏及IHA诊断液制备

›› 2011, Vol. 38 ›› Issue (7): 224-227.

辽西地区鸡大肠杆菌分离鉴定、药敏及IHA诊断液制备

王书全

辽宁医学院畜牧兽医学院,辽宁锦州 121001

收稿日期:2010-12-01 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20

Isolation and Identification of Chicken Escherichia coli and Drug Sensitive Test and the Preparation of IHA Diagnostic Solution in Western of Liaoning Province

WANG Shu-quan

College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121001,China

Received:2010-12-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20

摘要/Abstract

摘要： 本试验从辽西地区部分养鸡场采集具有典型临诊特征的鸡大肠杆菌病的病、死鸡病料,进行细菌分离培养和鉴定,分离到89株鸡大肠杆菌。通过血清型鉴定,鉴定出血清型的82株,血清型9种,分别为O₁、O₂、O₈、O₁₅、O₁₈、O₃₅、O₇₈、O₁₁₄、O₁₄₁,其中O₁₈、O₃₅型鸡大肠杆菌为辽西地区优势流行血清型。将分离纯化的O₃₅型鸡大肠杆菌超声裂解后,加入致敏双醛化处理过的红细胞,制备O₃₅型鸡大肠杆菌间接血凝诊断液,验证其特异性、敏感性和稳定性,完成诊断液的标准化。同时采用Kirby-Bauer纸片法,选择12种标准药敏片对此89株分离菌株进行药敏试验。结果表明,间接血凝诊断液特异性和敏感性好,质量稳定。而药敏试验结果显示,各地区均有耐药菌株的出现,仅对氟苯尼考、头孢噻呋、左旋氧氟沙星、盐酸沙拉沙星这4种药物敏感性较高。

关键词: 鸡大肠杆菌; 分离鉴定; 血清型; IHA诊断液; 药敏试验

Abstract: Chicken Escherichia coli samples were collected from the patient or dead chickens with typical clinical symptoms in western Liaoning province in the test, chicken Escherichia coli was isolated,cultured and identificated, 89 strains of chicken Escherichia coli were isolated. Identified 82 strains, belonging to 9 serotypes, they were O₁,O₂,O₈,O₁₅,O₁₈,O₃₅,O₇₈,O₁₁₄,O₁₄₁ serotypes respectively, O₃₅ and O₁₈ were predominant serotypes of chicken Escherichia coli in the western of Liaoning province. E.coli that was isolated and purified was cracked by supersonic wave,add to the double-aldehyde sensitization red blood cell, and made up of E.coli O₃₅ IHA diagnostic reagents, the specificity, sensitivity and stability were verified.Kirby-Bauer method was used as drug sensitive test to the 89 strains, and 12 standard drug sensitive slips were selected in the test. The results showed that the indirect hemagglutination diagnostic solution was specificity and sensitivity, and the resistant strains were detected in all region, just Forfenicol, Ceftiofur, Levofloxacin and Sarafloxacin were sensitive to chicken Escherichia coli.

Key words: chicken Escherichia coli; isolation and identification; serotypes; IHA diagnostic solution; drug sensitive test

中图分类号:

S852.61⁺2

王书全. 辽西地区鸡大肠杆菌分离鉴定、药敏及IHA诊断液制备[J]. , 2011, 38(7): 224-227.

WANG Shu-quan. Isolation and Identification of Chicken Escherichia coli and Drug Sensitive Test and the Preparation of IHA Diagnostic Solution in Western of Liaoning Province[J]. , 2011, 38(7): 224-227.

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