GnRH脉冲对体外原代培养大鼠GTH细胞中 LHβ mRNA表达水平的影响

›› 2011, Vol. 38 ›› Issue (4): 55-58.

GnRH脉冲对体外原代培养大鼠GTH细胞中 LHβ mRNA表达水平的影响

王新^1,2, 谭建华³, 赖小平², 万忠海³, 韦旭斌⁴

1. 青岛农业大学动物科学技术学院,山东青岛 266109;2. 广州中医药大学中药学院,广东广州 510006;3. 中国人民解放军军事医学科学院军事兽医研究所,吉林长春 130062;4. 吉林大学畜牧兽医学院,吉林长春 130062

收稿日期:2010-10-11 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20

The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats’ GTH Cells Primarily Cultured in vitro

WANG Xin^1,2, TAN Jian-hua³, LAI Xiao-ping², WAN Zhong-hai³, WEI Xu-bin⁴

1. College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;2. Traditional Chinese Medical Academy,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;3. Institute of Military Veterinary Academy of Military Medical Science,PLA,Changchun 130062,China;4. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China

Received:2010-10-11 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要： 研究不同脉冲频率GnRH对GTH细胞分泌LH的影响。利用细胞体外培养技术结合荧光定量PCR技术,对体外培养的大鼠原代垂体细胞在不同脉冲频率GnRH刺激下,细胞LHβ mRNA的变化进行了研究。在相同振幅条件下,高频刺激(30 min间隔)时,LHβ mRNA表达水平达到高峰。结果表明,高频脉冲主要引起LH的表达,支持了GnRH脉冲频率本身就是一个调控信号的假说。

关键词: GnRH; GTH; LHβ mRNA

Abstract: To explain at molecular level the influence of GnRH at different impulse frequencies on the secretion of LH by GTH.Research the change of LHβ mRNA in cells when the pituicytes of original generation of rats cultured in vitro were stimulated by GnRH at different impulse frequencies, by applying the technique of culture in vitro of cells in combination with the fluorescent quantitation of PCR.The result showed that in the condition of the same vibration amplitude, when the cells were stimulated by impulse at high frequency (at the interval of 30 min), the level of LHβ mRNA reached the top.This supports the hypothesis that GnRH impulse frequency itself is a regulation signal,and GnRH impulse at high frequency brings about mainly the response of LHβ.

Key words: GnRH; GTH; LHβ mRNA

中图分类号:

S852.2

王新;谭建华;赖小平;万忠海;韦旭斌. GnRH脉冲对体外原代培养大鼠GTH细胞中 LHβ mRNA表达水平的影响[J]. , 2011, 38(4): 55-58.

WANG Xin;TAN Jian-hua;LAI Xiao-ping;WAN Zhong-hai;WEI Xu-bin. The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats’ GTH Cells Primarily Cultured in vitro[J]. , 2011, 38(4): 55-58.

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