流式细胞术检测不同试验条件对猪T 淋巴细胞亚群的影响

›› 2011, Vol. 38 ›› Issue (4): 101-104.

流式细胞术检测不同试验条件对猪T 淋巴细胞亚群的影响

屈雪琪¹, 赵建增^1,2, 梁智选³, 李勇¹, 白雪¹, 梅林¹, 温涛², 何斌¹, 杨举¹, 罗胜军⁴, 邢海云², 高英杰¹

1. 吉林大学畜牧兽医学院,吉林长春 130062;2. 北京必威安泰生物科技有限公司,北京 100176;3. 天津市动物疫病预防控制中心,天津 300402;4. 广东省农业科学院兽医研究所,广东广州 510640

收稿日期:2010-10-26 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20
通讯作者: 高英杰

The Effects of T Lymphocyte Subset Detection for Flow Cytometry under Different Experimental Conditions

QU Xue-qi¹, ZHAO Jian-zeng^1,2, LIANG Zhi-xuan³, LI Yong¹, BAI Xue¹, MEI Lin¹, WEN Tao², HE Bin¹, YANG Ju¹, LUO Sheng-jun⁴, XING Hai-yun², GAO Ying-jie¹

1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Beijing Bigvet Biotech Co.Ltd.,Beijing 100176,China;3. Tianjin Center for Disease Control and Prevention,Tianjin 300402,China;4. Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China

Received:2010-10-26 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要： 通过不同方式处理猪外周血淋巴细胞,对不同保存时间的淋巴细胞进行流式细胞术检测,探讨其对T淋巴细胞亚群中CD3^＋、CD4^＋、CD8^＋亚群的影响。采用肝素钠、EDTA、枸橼酸钠3种抗凝剂抗凝猪外周静脉血,分别在第1、2、4、6、8、10天用流式细胞仪检测。结果表明,肝素钠抗凝,第1、2、4天内检测差异不显著(P＞0.05),第6天检测CD4^＋、CD8^＋细胞显著减少(P＜0.05),第8、10天极显著减少(P＜0.01)。EDTA抗凝,第1、2、4天内检测差异不显著(P＞0.05),第6天检测CD3^＋细胞显著减少(P＜0.05),第8、10天极显著减少(P＜0.01);枸橼酸钠抗凝,第1、2、4、6、8天内检测差异不显著(P＞0.05),第10天检测CD3^＋、CD4^＋细胞极显著减少(P＜0.01),CD8^＋细胞显著减少(P＜0.05)。3种抗凝剂检测比较,处理后的第1、2、4、6天肝素钠抗凝管检测结果CD3^＋、CD4^＋、CD8^＋均高于EDTA和枸橼酸钠抗凝。本研究表明,流式细胞术检测猪外周血优选肝素钠抗凝,样本采集后4 d内检测完毕。

关键词: 流式细胞术; T淋巴细胞亚群; 抗凝剂; 样本采集

Abstract: The experiments were designed to study the changed of T lymphocyte subset about CD3^＋,CD4^＋,CD8^＋ which detected by triple-color flow cytometry for swine,the blood sample were collected by three different anticoagulants and detection on different days. The results showed that CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd,4th day had no significant difference,but on the 6th CD4^＋,CD8^＋ had significant different,on the 8th day and 10th day had extremely significant different in the heparin anticoagulant samples group. In the EDTA anticoagulant samples group CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd and 4th day had no significant difference,on the 6th day CD3^＋ had significant difference,on the 8th and 10th day had extremely significant difference. In the sodium citrate anticoagulant samples group CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd,3rd,4th,6th day and the 8th day had no significant difference,on the 10th day CD3^＋,CD4^＋ had extremely significant difference,and CD8^＋ had significant difference.Compared three different anticoagulants,the heparin was better others,and it’s better to detect the samples within four days.

Key words: flow cytometry; T lymphocyte subset; anticoagulants; sample collection

中图分类号:

S852.4

屈雪琪;赵建增;梁智选;李勇;白雪;梅林;温涛;何斌;杨举;罗胜军;邢海云;高英杰. 流式细胞术检测不同试验条件对猪T 淋巴细胞亚群的影响[J]. , 2011, 38(4): 101-104.

QU Xue-qi;ZHAO Jian-zeng;LIANG Zhi-xuan;LI Yong;BAI Xue;MEI Lin;WEN Tao;HE Bin;YANG Ju;LUO Sheng-jun;XING Hai-yun;GAO Ying-jie. The Effects of T Lymphocyte Subset Detection for Flow Cytometry under Different Experimental Conditions[J]. , 2011, 38(4): 101-104.

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