青霉素钠完全抗原制备及其抗体水平检测

›› 2011, Vol. 38 ›› Issue (2): 193-196.

青霉素钠完全抗原制备及其抗体水平检测

杜军，贾芳，张玉彦，周学章

（宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川 750021）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-20 发布日期:2011-02-20
通讯作者: 周学章

Preparation of Sodium Penicillin Complete Antigen and Detection of the Antibody Level

DU Jun,JIA Fang,ZHANG Yu-yan,ZHOU Xue-zhang

(Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China)

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
Contact: ZHOU Xue-zhang

摘要/Abstract

摘要： 利用戊二醛法偶联青霉素钠（Penicillin,PEN）与BSA和OVA，制备完全抗原PEN-BSA和PEN-OVA。经紫外扫描及SDS-PAGE电泳鉴定完全抗原PEN-BSA和PEN-OVA偶联成功，并进行动物免疫制备抗血清，建立间接ELISA法测定免疫血清效价，经测定抗原PEN-BSA和PEN-OVA均达到理想的免疫效价，为进一步制备青霉素钠单克隆抗体及其青霉素钠药物残留快速检测试剂盒奠定基础。

关键词: 青霉素钠; 完全抗原; BSA; OVA

Abstract: Glutaraldehyde method was used in this study to conjugate sodium Penicillin and bovine serum albumin (BSA) and ovalbumin (OVA) so as to synthetic complete antigen PEN-BSA and PEN-OVA. The UV scanning and SDS-PAGE gel electrophoresis were used to identify whether sodium Penicillin protein-coupled with the carrier successly. BALB/c mice were immunized，the titre of antiserum was detected by indirect ELISA. High-titer had been produced in all immunized BALB/c mice which made it possible to establish for further preparation of monoclonal antibodies and quickly immuno-assay kit of PEN residues.

Key words: sodium Penicillin; complete antigen; BSA; OVA

中图分类号:

R927

杜军;贾芳;张玉彦;周学章. 青霉素钠完全抗原制备及其抗体水平检测[J]. , 2011, 38(2): 193-196.

DU Jun;JIA Fang;ZHANG Yu-yan;ZHOU Xue-zhang. Preparation of Sodium Penicillin Complete Antigen and Detection of the Antibody Level[J]. , 2011, 38(2): 193-196.

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