›› 2011, Vol. 38 ›› Issue (2): 165-167.

• 疾病防治 • 上一篇    下一篇

2010年上半年11省市规模猪场疫病监测与剖析

吕超超，焦连国，张金双，张永灯，赵锐，闫国晖，赵亚荣   

  1. （大北农动物医学研究中心，北京 100097）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-20 发布日期:2011-02-20

Laboratory Detection and Analysis of Common Viral Pathogens of Pig Farms in 11 Provinces during the First Half of 2010 Year

LV Chao-chao, JIAO Lian-guo, ZHANG Jin-shuang, ZHANG Yong-deng,ZHAO Rui, YAN Guo-hui, ZHAO Ya-rong   

  1. (Dabeinong Research Center for Veterinary Medicine, Beijing 100097, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20

摘要： 为了解近期发病猪场几种病毒性疫病的流行状况，采用RT-PCR和PCR方法，对2010年1月至6月期间，来自11省(市) 50个猪场共147份病料进行猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）、高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）、伪狂犬病毒（PRV）和猪圆环病毒2型（PCV2）检测，并采用ELISA方法对其中26个猪场458份血清样品进行伪狂犬gE野毒抗体检测。结果表明，PCV2阳性率最高，为75.3%（70/93）；PRRSV阳性率次之，为57.4%（78/136），HP-PRRSV阳性率为43.5%（54/124）；CSFV和PRV的阳性率分别为37.7%（52/138）和21.0%（21/100）；伪狂犬gE野毒抗体阳性率为16.4%（75/458）。同时检出2种及2种以上病毒的样品占46.9%（69/147），其中以HP-PRRSV+PCV2、CSFV+PCV2及CSFV+HP-PRRSV+PCV2三种形式最为常见。PRRSV（50.0%）是猪场繁殖障碍性疾病中的最常见病原；CSFV（56.0%）和PCV2（48.0%）是断奶前后仔猪腹泻的常见病原；HP-PRRSV（53.5%）、CSFV（50.7%）和PCV2（48.0%）是保育猪发生高热症的常见病原。该检测结果为猪场疫病的诊断与防控提供了参考。

关键词: 猪瘟; 猪繁殖与呼吸综合征; 伪狂犬病; 猪圆环病毒病; PCR; ELISA; 检测猪瘟; 猪繁殖与呼吸综合征; 猪圆环病毒病; PCR; ELISA; 检测

Abstract: To understand the recent prevalence of several viral diseases in sick pig farms, 147 tissue specimens were collected from 50 farms in 11 provinces (cities) and examined during January 2010 to May 2010 by RT-PCR and PCR methods. And 458 serum samples from 26 farms were examined for pseudorabies gE antibodies by ELISA method. The positive rate of porcine circovirus type 2 (PCV2) was highest with 75.3% (70/93). The positive rate of porcine reproductive and respiratory syndrome virus (PRRSV) followed with 57.4% (78/136), and that of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) was 43.5% (54/124). The positive rates of classical swine fever virus (CSFV) and pseudorabies virus (PRV) were 37.7% (52/138) and 21.0% (21/100), respectively. The positive rate of pseudorabies gE antibody was 16.4% (75/458). 46.9% (69/147) of samples including two and more than two species pathogens were detected, and HP-PRRSV+PCV2, CSFV+PCV2, and CSFV+HP-PRRSV+PCV2 were three forms of the most common. PRRSV (50.0%) was the most common cause of pig reproductive disorders; CSFV (56.0%) and PCV2 (48.0%) were the common causes of diarrhea in the pre /post-weaning; HP-PRRSV (53.5%), CSFV (50.7%) and PCV2 (48.0%) were commonly detected in nursery pigs with high fever syndrome. The results provided references for the diagnosis, prevention and control of swine diseases.

Key words: CSF; PRRS; PR; PCV; PCR; ELISA; detection

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