›› 2011, Vol. 38 ›› Issue (2): 132-135.

• 遗传繁育 • 上一篇    下一篇

吉林白鹅类胰岛素生长因子Ⅱ的SNP及其与屠体性状的相关性分析

徐晶1,2,单雪松2   

  1. (1.吉林农业大学发展学院,吉林长春 130600; 2.吉林农业大学动物科技学院,吉林长春 130118)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-20 发布日期:2011-02-20
  • 通讯作者: 单雪松

Study on IGFⅡ Gene SNP and its Effect on Meat Traits in Jilin White Goose

XU Jing1,2,SHAN Xue-song2   

  1. (1.Development Institute of Jilin Agricultural University, Changchun 130600, China;2.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
  • Contact: SHAN Xue-song

摘要: 试验设计了5对引物,采用PCR-SSCP方法对吉林白鹅肉用品系85只个体类胰岛素生长因子Ⅱ(IGFⅡ)进行了SNP检测,分析其群体遗传结构,并对SNP与屠体性状的相关性进行了研究。结果表明,在IGFⅡ外显子3中存在3种基因型AA、AB、BB基因型,基因型频率分别为0.7647、0.1412、0.0941,AA为优势基因型;存在2种等位基因A、B,基因频率分别为0.8353、0.1647,A为优势等位基因。IGFⅡ外显子3中发现1处SNP位点(A→G),该突变为沉默突变,未引起氨基酸序列改变。对3种基因型进行方差分析,结果发现,BB基因型个体半净膛重、肝脏重显著高于AA、AB基因型(P<0.05);腿肉重BB基因型极显著高于AA基因型(P<0.01);基因型对全净膛重、翅重、胸肉重无显著影响(P>0.05)。

关键词: 鹅; IGFⅡ; SNP; 肉用性状

Abstract: Five primers were designed to amplify IGFⅡ gene and SNPs were detected by PCR-SSCP in 85 Jilin white goose meat stock population. The population genetic construction and the correlation between SNPs and carcass traits were analyzed. The results of PCR-SSCP showed that there were 3 genotypes of AA, AB, BB in exon 3 region of IGFⅡ, the genotypic frequency were 0.7647, 0.1412 and 0.0941, respectively,AA was dominance genotypes. There were 2 allele genes of A,B, gene frequency were 0.8353 and 0.1647, respectively, A was dominance allele gene. One SNP was found in
exon 3 region of IGFⅡ (A→G), sequence analogy indicated that it was a silent mutation, and the amino sequence was not change. By variance analysis for three genotypes, AA, AB genotypes were significant lower than BB genotype for half wipe chest weight and liver weight (P<0.05), AA genotype were significant lower than BB genotype for leg weight (P<0.01). There were no significant level for whole wipe chest, chest weight and wing weight(P>0.05).

Key words: goose; IGFⅡ; SNP; meat trait

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