用乙基环丙沙星重建受猪鼻支原体污染的BHK-21细胞的研究

›› 2011, Vol. 38 ›› Issue (12): 45-49.

用乙基环丙沙星重建受猪鼻支原体污染的BHK-21细胞的研究

高林, 廖德芳, 姚俊, 肖雷, 李华春, 张念祖, 李志华

云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室农业部热带亚热带动物病毒学重点开放实验室,云南昆明 650224

收稿日期:2011-04-11 修回日期:1900-01-01 出版日期:2011-12-20 发布日期:2011-12-20
通讯作者: 李华春

To Reconstruct the Contaminated BHK-21 Cells by M.hyorhinis with Enrofloxacin

GAO Lin, LIAO De-fang, YAO Jun, XIAO Lei, LI Hua-chun, ZHANG Nian-zu, LI Zhi-hua

Key Laboratory of Tropical & Subtropical Animal Virology of the Ministry of Agriculture,Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China

Received:2011-04-11 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

摘要/Abstract

摘要： 为重建受猪鼻支原体人为污染的BHK-21细胞,本研究采用含10 mg/L乙基环丙沙星的培养液,对受污染的BHK-21细胞进行物理、化学相结合的循环处理,并用DNA荧光染色法和PCR法对处理进行全程监测。结果发现,4次循环处理并再传3代后,猪鼻支原体污染已被彻底清除,细胞恢复了健康。此方法为抢救受支原体污染的细胞提供了参考。

关键词: 乙基环丙沙星; 猪鼻支原体; BHK-21细胞; 清除污染

Abstract: For reconstructing the contaminated BHK-21 cells artificially by M.hyorhinis with the Enrofloxacin, the contaminated BHK-21 cells were treated with the MEM growing solution of 10 mg/L Enrofloxacin circularly by physical and chemical methods, and the course was monitored by the methods of DNA fluorescent staining and PCR. The M.hyorhinis was cleaned successfully after 4 cycles and 3 passages, the BHK-21 cells were recovered. The method offered a reference for saving the contaminated cells by Mycoplasma.

Key words: Enrofloxacin; M.hyorhinis; BHK-21 cells; clean contamination

中图分类号:

Q2-33

高林;廖德芳;姚俊;肖雷;李华春;张念祖;李志华. 用乙基环丙沙星重建受猪鼻支原体污染的BHK-21细胞的研究[J]. , 2011, 38(12): 45-49.

GAO Lin;LIAO De-fang;YAO Jun;XIAO Lei;LI Hua-chun;ZHANG Nian-zu;LI Zhi-hua. To Reconstruct the Contaminated BHK-21 Cells by M.hyorhinis with Enrofloxacin[J]. , 2011, 38(12): 45-49.

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