›› 2011, Vol. 38 ›› Issue (10): 221-224.

• 经验交流 • 上一篇    下一篇

猪胸膜肺炎放线杆菌四川株的分离鉴定及药物敏感性试验

彭娟1, 杨泽晓1, 宋勇1, 王印1,2   

  1. 1. 四川农业大学动物医学院,四川雅安 625014;2. 动物疫病与人类健康四川省重点实验室,四川雅安 625014
  • 收稿日期:2011-04-16 修回日期:1900-01-01 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 王印

The Isolation and Drug Susceptibility Test of Actinobacillus pleuropneumoniae Sichuan Isolates

PENG Juan1, YANG Ze-xiao1, SONG Yong1, WANG Yin1,2   

  1. 1. College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014, China;2. Key Laboratory of Animal Disease and Human Health of Sichuan Province,Ya'an 625014, China
  • Received:2011-04-16 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

摘要: 从四川某猪场疑似胸膜肺炎放线杆菌感染的病料中分离到一株革兰氏阴性小球杆菌。经染色镜检、生化试验、PCR检测、血清型检测,致病性试验和药敏试验,鉴定该菌为猪胸膜肺炎放线杆菌1型,且具有较强致病性。药敏试验结果表明,该菌对四环素、甲氧苄啶,以及链霉素、庆大霉素、卡那霉素、丁胺卡那、新霉素等氨基糖苷类药物高度耐药,对氯霉素、氟苯尼考、氨苄西林、阿莫西林、羧苄西林和恩诺沙星、诺氟沙星等喹诺酮类受试药物敏感。

关键词: 胸膜肺炎放线杆菌; 分离; 鉴定; 药敏试验

Abstract: 1 strain Actinobacillus pleuropneumoniae(APP) serotype 1 G- coccobacillus,which with high virulence,was isolated from samples of sick pigs suspected pleuropneumonia infection collected from a pig farm in Sichuan province,and was identified through the bacterial colony,bacteria morphological observation,biochemistry reaction,PCR identification,serological test,pathogenicity test,and drug susceptibility test. The drug susceptibility test results showed that the APP Sichuan isolates were resistance to tetracycline,trimethoprim and Streptomycin,Gentamicin,Kanamycin,Amikacin,etc. Aminoglycosides,but sensitive to chloramphenicol,Ampicilllin,Amoxicillin,Carbenicillin,and some quinolones drugs used in this study.

Key words: Actinobacillus pleuropneumoniae; isolation; identification; drug susceptibility test

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