应用实时荧光定量PCR测定牛体细胞端粒长度

›› 2010, Vol. 37 ›› Issue (9): 154-157.

应用实时荧光定量PCR测定牛体细胞端粒长度

吕昆，林丹，许淼，朱怡文，黄英

（上海交通大学医学院，上海市儿童医院上海医学遗传研究所，卫生部医学胚胎分子生物学重点实验室，上海市生殖与胚胎工程重点实验室，上海 200040）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
通讯作者: 黄英

Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR

LV Kun，LIN Dan，XU Miao，ZHU Yi-wen，HUANG Ying

（Shanghai Institution of Medical Genetics, Shanghai Children’s Hospital，Shanghai Jiao Tong University School of Medicine，Key Laboratory of Embryo Molecular Biology，Ministry of Health; Shanghai Laboratory of Embryo and Reproduction Engineering，Shanghai 200040，China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: HUANG Ying

摘要/Abstract

摘要： 本研究为了验证应用实时荧光定量聚合酶链反应（quantitative PCR，Q-PCR）测定牛基因组端粒长度的可行性，选取18个不同来源牛耳成纤维细胞抽提基因组DNA为样本，对Q-PCR和经典的Southern印迹法进行了相关性分析。结果显示，Q-PCR测定端粒长度相对T/S为1.16±0.24，Southern印迹法测量端粒平均TRF值为16.99 kb±0.85 kb，两种方法获得的结果相关性分析R2=0.5612(P＜0.01)，因此实时荧光定量PCR是一种测定牛基因组端粒长度的可靠的方法。

关键词: 荧光定量聚合酶链式反应; 印迹法; 牛体细胞; 端粒长度

Abstract: In order to test the possibility of quantitative PCR (Q-PCR) in the measurement of bovine telomere length, we chose 18 different DNA samples of bovine ear fibroblast, and then analyzed the relativity of quantitative PCR and Southern blotting methods. The results showed that the relative T/S ratio of Q-PCR was 1.16±0.24, the mean TRF of Southern blotting was 16.99 kb±0.85 kb.The analysis of the relativity of two methods showed that R2= 0.5612(P<0.01), so we got the conclusion that Q-PCR was a reliable method in the measurement of bovine telomere length.

Key words: quantitative PCR; Southern blotting; bovine somatic cell; telomere length

中图分类号:

S814.8

吕昆;林丹;许淼;朱怡文;黄英. 应用实时荧光定量PCR测定牛体细胞端粒长度[J]. , 2010, 37(9): 154-157.

LV Kun;LIN Dan;XU Miao;ZHU Yi-wen;HUANG Ying. Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(9): 154-157.