甲氧苄啶对蒲公英体外抗菌增效作用的研究

›› 2010, Vol. 37 ›› Issue (2): 169-171.

甲氧苄啶对蒲公英体外抗菌增效作用的研究

王新，崔一喆，邵红，韩铁锁

（黑龙江八一农垦大学动物科技学院，大庆 163319）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-20 发布日期:2010-02-20

Study on Synergitic Antibacteria Actions of TrimethoprimCombined with Herba Taraxaci in vitro

WANG Xin, CUI Yi- zhe, SHAO Hong, HAN Tie-suo

(Animal Sciences and Technology College of Heilongjiang August 1st Land Reclamation University, Daqing 163319,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

摘要/Abstract

摘要： 摘要：采用微量棋盘稀释法测定了蒲公英、甲氧苄啶及两药联用对金黄色葡萄球菌、大肠杆菌和沙门氏菌的最小抑菌浓度（MIC），计算蒲公英与甲氧苄啶联用的组分抑菌浓度（FIC）指数。结果表明，蒲公英与甲氧苄啶联用对金黄色葡萄球菌、大肠杆菌和沙门氏菌的FIC指数分别为0.75、0.75和1，呈相加作用。采用平板计数的方法，测定了蒲公英与甲氧苄啶联用作用于3种细菌1、2、4和8 h后的细菌数，并计算杀菌率。利用最小二乘法对杀菌率进行拟合，计算出甲氧苄啶增强蒲公英体外抗金黄色葡萄球菌、大肠杆菌和沙门氏菌作用的最优添加剂量分别为4.0、1.8和2.0 mg/g。

关键词: 关键词：蒲公英; 甲氧苄啶; 抗菌增效作用

Abstract: Abstract：The minimum inhibitory concentrations (MIC) of Herba Taraxaci, trimethoprim and Herba Taraxaci combined with Trimethoprim against Staphylococcus aureus, Escherichia coli and Salmonella were measured individually by the method of microdilution checkerboard techniques. Then the fractional inhibitory concentrations (FIC) indices of Herba Taraxaci combined with Trimethoprim were calculated. The results showed that the FIC values on Staphylococcus aureus, Escherichia coli and Salmonella were respectively 0.75, 0.75,1. The effect of their combination taken on additive action. The number of Staphylococcus aureus, Escherichia coli and Salmonella which had been inhabited by Herba Taraxaci combined with Trimethoprim for 1 h, 2 h, 4 h and 8 h, were enumerated by plate counts methods. Then the killing rate of Herba Taraxaci combined with Trimethoprim were calculated. The experimental data were fitted by least squares method. The optimal dose of Trimethoprim which combined with Herba Taraxaci against Staphylococcus aureus, Escherichia coli and Salmonella in vitro, were respectively 4.0 mg/g,1.8 mg/g and 2.0 mg/g. 

Key words: Key words：Herba Taraxaci; Trimethoprim; synergistic antibacterial action

王新;崔一喆;邵红;韩铁锁. 甲氧苄啶对蒲公英体外抗菌增效作用的研究[J]. , 2010, 37(2): 169-171.

WANG Xin;CUI Yi- zhe;SHAO Hong;HAN Tie-suo. Study on Synergitic Antibacteria Actions of TrimethoprimCombined with Herba Taraxaci in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(2): 169-171.

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