›› 2010, Vol. 37 ›› Issue (10): 105-108.

• 生物技术 • 上一篇    下一篇

CAPN3基因在草原红牛不同组织中的表达差异

刘博洋1, 秦立红1, 廉传江1, 田静1, 张金玉1, 姜昊2, 赵志辉1, 杨润军1   

  1. (1.吉林大学畜牧兽医学院, 长春 130062;2.吉林大学实验动物中心, 长春 130062)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 杨润军

Expression and Comparative Study of Calpain3 (CAPN3) Gene in Different Tissues of Chinese Red Steppe

LIU Bo-yang1, QIN Li-hong1, LIAN Chuan-jiang1, TIAN Jing1, ZHANG Jin-yu1, JIANG Hao2, ZHAO Zhi-hui1, YANG Run-jun1   

  1. (1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2.Centre of Experimental Animal, Jilin University, Changchun 130062, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: YANG Run-jun

摘要: 钙激活中性蛋白酶(CAPN3)参与哺乳动物体内多种重要的生理生化过程,但是有关它的确切生理功能和作用机制研究较少。本研究采用实时荧光定量PCR技术检测CAPN3基因在草原红牛多种组织中的表达水平差异情况。试验结果表明,CAPN3基因在所检测草原红牛的心脏、肝脏、脾脏、肺脏、肾脏、十二指肠、瘤胃和背最长肌8种组织中均有表达,且在不同组织中的表达水平有所不同。草原红牛脾脏中的CAPN3基因表达量显著高于其他组织,十二指肠和瘤胃中的CAPN3基因表达量显著高于心脏、肝脏、肺脏、肾脏和背最长肌。本试验为肉牛肉质性状的改良提供了分子遗传学依据,并为深入研究草原红牛体内CAPN3基因的生物学作用及作用机制奠定了基础。

关键词: 草原红牛; CAPN3; 实时荧光定量PCR; 表达水平

Abstract: The present study was designed to detect the differences of the calpain 3 (CAPN3) gene expression level in varieties tissues of Chinese Red Steppe by real-time RT-PCR. The results showed that CAPN3 mRNA was expressed in all of the eight tissues: heart, liver, spleen, lung, kidney, intestine, stomach and skeletal muscle; we also found that CAPN3 gene expression level was higher in spleen than in other tissues of Chinese Red Steppe (P<0.05). The research provides the basis for the future studies of the biological function and the molecular mechanisms of CAPN3 gene.

Key words: Chinese Red Steppe;  CAPN3; real-time PCR; expression level

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