›› 2009, Vol. 36 ›› Issue (5): 45-48.

• 生理生化 • 上一篇    下一篇

牛瘤胃液中链球菌的分离与鉴定

苑学1,2,邢欣1,龙淼1,逄晓阳1,3,刘国文1,孙国权2,王哲1   

  1. (1.吉林大学畜牧兽医学院,长春 130062; 2.内蒙古民族大学动物科技学院,通辽 028042;3.吉林省产品质量监督检验院,长春 130022)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 刘国文

Isolation and Identification of Streptococcus Bovis in Cattle Rumen Gastric Juice

YUAN Xue1,2,XING Xin1, LONG Miao1,PANG Xiao-yang1,3,LIU Guo-wen1,SUN Guo-quan2,WANG Zhe1   

  1. (1.College of Animal Science and Veterinary Medicine, Jilin University,Changchun 130062,China;2. College of Animal Science and Technology, Inner Mongolia University for the Nationalities,Tongliao 028042,China;3. Product Quality Supervision and Inspection Institute of Jilin Province, Changchun 130022,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: LIU Guo-wen

摘要: 在牛瘤胃中,牛链球菌的数量和发酵类型直接影响瘤胃的正常生理活动,同时牛链球菌发酵产生的乳酸和其内存在的各种酶在实际的工业生产中具有重要的价值。本试验从新采集的牛瘤胃液中分离得到菌株,用微量生化反应管和16S rRNA技术分别对菌株的生化及遗传特性进行鉴定,结合革兰氏染色和形态学观察结果判定所分离的菌株即为牛链球菌,为更好地研究和改造该菌奠定了理论和试验基础。

关键词: 牛链球菌; 分离鉴定; 16S rRNA

Abstract: n cattle rumen,the number of Streptococcus bovis and the type of fermentation have a direct effect on ruminal normal physiological activity.In the mean time, lactic acid which is produced by Streptococcus bovis’s fermentation and all kinds of enzymes which exist in Streptococcus bovis have important values in practical industrial production. In this test, author isolated strain from ruminal fluids,and used the microcrystalline biochemical event reactons and the 16S rRNA gene technology to identify the biochemistry and inherited characteristic of the isolated strain respectively, binding to the viewing result of gram staining and morphology, author assessment the isolated strain was Streptococcus bovis, providing the theory and experiment foundation for study and research the streptococcus bovis preferably.

Key words: Streptococcus bovis; isolation and identification; 16S rRNA

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