›› 2008, Vol. 1 ›› Issue (9): 34-37.

• 生物技术 • 上一篇    下一篇

RNA病毒感染性分子克隆的研究

钱淑凤, 郝玉兰, 郑翠玲   

  1. 唐山职业技术学院环境工程系, 唐山 063004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-20 发布日期:2008-09-20

Study on Infectious Molecular Clone of RNA Virus

QIAN Shufeng,HAO Yulan, ZHENG Cuiling   

  1. Tangshan Vocational Technical College, Tangshan 063004,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

摘要: 随着反向遗传学技术的兴起和发展,RNA分子体外操作已经成为可能,并且技术日臻完善。 反向遗传操作技术的关键就是构建RNA病毒的感染性分子克隆,在DNA分子水平上对其进行体外操作,从而研究病毒结构与功能的方法。作者对感染性克隆的构建方法及感染性体外转录方法作一综述。 

关键词: RNA病毒; 感染性克隆; 感染性体外转录本

Abstract: Following the rise and developmemt of reverse genetics technique, it become possible to work with RNA molecule in vitro.The construction infectious molecular clone of RNA virus and operation in DNA moleculear level in vitro is the key to reverse genetics technique. The virus's structure and function can be studied by this way. In this articles the way of constructing infectious clone and in vitro transcription were reviewed.

Key words: RNA病毒; 感染性克隆; 感染性体外转录本

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