›› 2008, Vol. 1 ›› Issue (2): 124-125.

• 兽医临床 • 上一篇    下一篇

青海省乌兰县牧羊犬犬新孢子虫病的血清学调查

牛小迎,马利青   

  1. 青海省畜牧兽医科学院,西宁 810016
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-02-20 发布日期:2008-02-20

Serodiagnosis of Neospora Caninum Infection in Shepherd Dogs of Wulan County of Qinghai Province

NIU Xiaoying,MA Liqing   

  1. Qinghai Academy of Animal and Veterinary Sciences,Xining 810016,China 
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-02-20 Published:2008-02-20

摘要: 用犬新孢子虫的重组蛋白GST-NcSAG1t作为ELISA诊断抗原,对青海省乌兰县的部分
牧羊犬进行了犬新孢子虫病的血清学调查。经过对所收集的80份牧羊犬血清的检测,共
检出犬新孢子虫病阳性血清25份,阳性率为31.25%。结果表明青海省乌兰地区的牧
羊犬中存在犬新孢子虫感染。

关键词: 犬新孢子虫; 重组蛋白GST-NcSAG1t; ELISA; 牧羊犬

Abstract: The purified GSTNcSAG1t was used in an enzymelinked immunosorbent assay (ELISA) for the detection of N caninum antibodies in shepherd dogs from western of Qinghai area. Field serum samples collected from shepherd dogs of Wulan County of Qinghai province were examined for the diagnosis of neosporosis by using the ELISA. Of the 80 analyzed samples from shepherd dogs 25(31.25%)samples were positive for antibodies to N caninum. These results suggest that Neospora caninum were infected in shepherd dogs from Wulan county of Qinghai province.

Key words: neospora caninum; GST-NcSAG1t; ELISA; shepherd dogs

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