›› 2007, Vol. 34 ›› Issue (5): 81-84.

• 疾病防治 • 上一篇    下一篇

小反刍兽疫快速诊断技术及其疫苗的研究进展

李刚,江彦增,晁生玉,张锦秀,朱鸿飞,时建忠
  

  1. 中国农业科学院北京畜牧兽医研究所,北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20

On Rapid Diagnosis and Vaccine Research of Peste Des Petits Ruminants

LI Gang,JIANG Yanzeng,CAO Shengyu,ZHANG Jinxiu,ZHU Hongfei,SHI Jianzhong
  

  1. Beijing Institute of Animal Science and Veterinary Medicine,CAAS,Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

摘要: 小反刍兽疫(PPR)是一种感染家养和野生反刍类动物的急性传染病,它是由副粘病毒科麻疹病毒属的小反刍兽疫病毒(PPRV)引起的。本病没有有效治疗方法,只能预防,因此诊断监测技术是控制该病的重要手段。由于传统的血清学检测技术,如琼脂糖凝胶免疫扩散(AGID)、病毒中和试验(VNT)等存在诸多缺陷,难以用于大规模的疫情监控。目前国际上发展的主流是以分子生物学技术为基础的检测方法,如RT-PCR、PCR-ELISA等,这些新技术灵敏便捷,高通量,而且能够在野外进行。而疫苗以重组疫苗为研究方向。

关键词: 小反刍兽疫; 病毒诊断

Abstract: Peste des petits ruminants (PPR) is a highly contagious and infectious viral disease of domestic and wild small ruminants included in the CLASS A list of OIE. It is caused by peste des petits ruminants virus (PPRV), a Morbillivirus in family Paramyxoviridae, closely related to renderpest virus (RPV). As there is no cure for this disease, diagnostic technology plays a very important role in disease control. But conventional ways like the agar gel immunodiffusion (AGID), virus neutralization test (VNT), et al. could work well in the surveillance of PPR. Now new assays based on molecular biology techniques , like the RT-PCR. PCR- ELISA is the main trend. They were sensitive, high throughput, time-saving, and could operation in the field. Recombinant vaccines are researched and used at present. 

Key words: PPR; PPRV diagnosis

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